THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
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A. Bajado-Gajutos, Assistant Professor College of Veterinary Medicine,<br />
University of Eastern Philippines, Catarman, Northern Samar<br />
The microscopic anatomy of the testes and epididymis of<br />
four Philippine-murrah crossbreds, three to four years of<br />
age, was studied. The connective tissue of the testes was<br />
made up mainly of collagen fibers, blood and lymphatic<br />
vessels of various sizes, nerves and fibroblasts. The<br />
parenchyma of the testes was composed of seminiferous<br />
tubules separated from each by loose connective tissue,<br />
the interstitium. The tubules were lined by spermatogenic<br />
and sertoli cells. The interstitium consisted mainly of reticular<br />
fibers, interstitial (leydig) cells, blood and lymphatic<br />
vessels, nerves and fibroblasts. The interstitial cells<br />
occurred singly or in groups near blood vessels. The<br />
efferent ductules were lined by low columnar epithelium<br />
consisting of columnar, basal and intraepithelial<br />
lymphocytes. The epididymal duct in the head, body and<br />
tail was lined by ciliated pseudostratified columnar epithelium<br />
consisting of tall columnar, basal, clear and intra-epithelial<br />
lymphocytes. The ducts contained spermatozoa of<br />
increasing volume from the head to the tail region.<br />
Keywords: microscopic anatomy, testis, epididymis,<br />
Philippine-murrah crossbred, Bubalus bubalis Linn.<br />
*MS Thesis, College of Veterinary Medicine, University<br />
of the Philippines Los Baños, College, Laguna (1999).<br />
Copy of the thesis is available at the library of the College<br />
of Veterinary Medicine, University of the Philippines Los<br />
Baños, College, Laguna. Part of the thesis was presented<br />
in the 70 th Philippine Veterinary Medical Association Annual<br />
Convention and Scientific Conference, February 19-21,<br />
2003 Bohol Tropics Resort, Tagbilaran, Bohol.<br />
A-24<br />
PALATINE PRINTING: AN ALTERNATIVE METHOD<br />
FOR <strong>THE</strong> IDENTIFICATION OF CARABAO (Bubalus<br />
bubalis L.)*<br />
A. S. Galicia , Student<br />
Palatine printing was performed in live carabaos. A palatine<br />
print showed the actual configuration of the rostral third of<br />
the hard palate. All the features of this part such as the<br />
transverse ridges, dental pads, median raphe and the<br />
incisive papilla were outlined in the prints. Upon closer<br />
examination, no two palatine prints were exactly alike.<br />
Prints taken by the same person from the same animal<br />
were identical. Also, two palatine prints taken from the<br />
same animal by different persons showed no difference.<br />
Problems encountered during the palatine printing process<br />
that have affected quality of the palatine prints included<br />
10<br />
ABSTRACT OF RESEARCHES ON<br />
<strong>THE</strong> <strong>PHILIPPINE</strong> <strong>WATER</strong> <strong>BUFFALO</strong><br />
movement of the animal, failure of the mouth speculum to<br />
adequately open the mouth of the animal, excessive<br />
salivation of the animal, uneven ink application on the<br />
mucosa, excessive salivation of the animal, uneven ink<br />
application on the mucosa, and uneven digital pressure<br />
during print taking. In spite of all these, however, the present<br />
study proved that palatine printing can be used as an<br />
alternative method of identification in the carabao.<br />
Keywords: palatine printing, method for identification,<br />
carabao, Bubalus bubalis<br />
* Undergraduate thesis, College of Veterinary Medicine,<br />
University of the Philippines Los Baños, 2002.<br />
A-25<br />
HISTOLOGY OF <strong>THE</strong> INTERSTITIAL TISSUE OF<br />
<strong>THE</strong> TESTES WITH SOME ULTRASTRUCTURAL<br />
OBSERVATIONS OF <strong>THE</strong> INTERSTITIAL (Leydig)<br />
CELLS OF <strong>THE</strong> <strong>PHILIPPINE</strong> CARABAO (Bubalus<br />
bubalis)*<br />
J. G. Inoc, Student<br />
C. P. Maala, Professor, College of Veterinary Medicine, University of the<br />
Philippines Los Baños<br />
The interstitial tissue and cells of the Philippine carabao<br />
were histologically and ultrastructurally characterized,<br />
respectively Collagen and reticular connective fibers were<br />
markedly abundant while elastic fibers were few. Extensive<br />
lymphatic vessels were present. Interstitial cells appeared<br />
in cluster of five to seven and were closely associated<br />
with the lymphatic vessels. Adjacent to the interstitial cells<br />
were vacuoles, arterioles and capillaries. Few fibroblast<br />
were also present. Ultrastructurally, the interstitial cells<br />
were irregularly polyhedral in shape with variably shaped<br />
nucleus. The nucleus was a ovoid and peripherally located<br />
while the nucleolus was small and eccentrically located.<br />
The hetero chromatin granules sometimes occurred in<br />
clumps, dispersed and closely associated with the nuclear<br />
membrane. The cytoplasm was filled with smooth<br />
endoplasmic reticulum large number of oval to round shape<br />
mitochondria and were scattered among the smooth<br />
endoplasmic reticulum. Their cristea were poorly defined<br />
but dense bodied within were observed. Rough<br />
endoplasmic reticulum, lipid droplets, golgi elements and<br />
membrane-bound bodies were visible in the cytoplasm. In<br />
general, the ultrastructural characteristics of the bubaline<br />
interstitial cells observed in the Philippine carabao were<br />
identical with what have been reported for other species of<br />
animals.<br />
Keywords: carabao bull, interstitial cells, testes,<br />
ultrastructural characteristics, Bubalus bubalis