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THE PHILIPPINE WATER BUFFALO

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Terminal Inc., abattoir, incidence of slaughtered pregnant<br />

ruminants<br />

*Abstract of Completed Researches, 1981-1996, (1990)<br />

PCC-DA, UPLB. Also in OED-PCC-DA Abstracts: Water<br />

Buffalo Researches in the Philippines 1981-1995, p.44-45.<br />

Copy of thesis available at the library of the College of<br />

Veterinary Medicine, University of the Philippines Los<br />

Baños.<br />

A-18<br />

HISTOLOGY OF <strong>THE</strong> SPLEEN OF <strong>PHILIPPINE</strong><br />

CARABAO (Bubalus bubalis L.)*<br />

M. A. C. Estacio, Assistant Professor, College of Veterinary Medicine,<br />

University of the Philippines Los Baños<br />

C. L. Calimlim, Student<br />

G. D. de Ocampo, Professor, College of Veterinary Medicine, University<br />

of the Philippines Los Baños<br />

The spleen of adult male Philippine carabao (Bubalus bubalis<br />

L.) was examined histologically using the H & E stain.<br />

The carabao had a storage type of spleen characterized by<br />

presence of numerous trabeculae and minimal amount of<br />

white pulp in the parenchyma. It was enclosed by a thick<br />

capsule consisting of two distinct layers: a thick outer<br />

dense white fibrous connective tissue layer (DWFCT) and<br />

an inner smooth muscle layer. The white pulp was made<br />

up of the lymphatic module and peripheral sheath that<br />

surrounded the nodular or central arteriole. The red pulp or<br />

splenic cord consisted of lymphocytes, phagocyte cells<br />

and erythrocytes separated by sinuses.<br />

Keywords: Bubalus bubalis, Philippine carabao, histology,<br />

spleen<br />

*The Philippine Journal of Veterinary Medicine, 34(1&2):<br />

65-67, 1997.<br />

A-19<br />

GROSS AND MICROSCOPIC ANATOMY OF <strong>THE</strong><br />

LUNG OF <strong>THE</strong> <strong>PHILIPPINE</strong> CARABAO (Bubalus<br />

bubalis Linn.)*<br />

A. C. Estacio, Assistant Professor, College of Veterinary Medicine,<br />

University of the Philippines Los Baños<br />

R. L. D. Hontomin, Student<br />

G. D. de Ocampo, Professor, College of Veterinary Medicine, University<br />

of the Philippines Los Baños<br />

The lung of the Philippine carabao was grossly and<br />

microscopically examined. Grossly, the left lung was<br />

smaller and had 2 lobes, cranial and caudal lobes. The<br />

cranial lobe was further divided into cranial and caudal<br />

8<br />

ABSTRACT OF RESEARCHES ON<br />

<strong>THE</strong> <strong>PHILIPPINE</strong> <strong>WATER</strong> <strong>BUFFALO</strong><br />

parts by a U or V shaped notch. The right lung had 4 lobes<br />

namely: cranial, middle, caudal and accessory lobes.<br />

Microscopically, the lung was enclosed by a capsule<br />

composed of 3 layers; mesothelium, elastic, and subserosal<br />

layers. The lung parenchyma presented structures typical<br />

of the lung of most domestic mammalian species. The wall<br />

of the bronchus consisted of 4 layers; 1) tunica mucosa<br />

which is made up of pseudostratified columnar ciliated<br />

lining epithelium with goblet cells, lamina propria containing<br />

loose connective tissue and lymphatic tissue, and a lamina<br />

muscularis mucosae of circulatory arranged smooth muscle<br />

fibers; 2) tunica submucosa made up of loose connective<br />

tissue, solitary lymphatic nodules and mixed but<br />

predominantly mucous glands; 3) tunica muscularis<br />

contained hyaline cartilage; and 4) tunica adventitia<br />

composed of loose connective tissue. The wall of the<br />

bronchiole, except that of the respiratory bronchiole, had 2<br />

layers: 1) tunica mucosa made up of simple columnar or<br />

cuboidal ephithelium, a lamina propria with thin loose<br />

connective tissue and lamina muscularis mucosae with<br />

thin circularly arranged smooth muscles; and 2) tunica<br />

submucosa with thin layer of loose connective tissue.<br />

Respiratory bronchioles were not frequently observed.<br />

The wall of the bronchial part consisted of 1 layer; tunica<br />

mucosa composed of simple cuboidal epithelium, lamina<br />

propria with very thin loose connective and lamina<br />

muscularis mucosae with thin smooth fiber. The alveolar<br />

part was lined by simple squamous epithelium. The alveolar<br />

duct, alveolar sac and alveoli were typical of other<br />

mammalian species.<br />

Keywords: alveoli, bronchiole, bronchus, bubaline,<br />

capsule, lung, Bubalus bubalis<br />

*The Philippine Journal of Veterinary Medicine, 36:1-7,<br />

1999.<br />

A-20<br />

ULTRASTRUCTURAL OBSERVATIONS OF <strong>THE</strong><br />

CECAL MUCOSA OF <strong>THE</strong> <strong>PHILIPPINE</strong> CARABAO<br />

(Bubalus bubalis) *<br />

M. A. C. Estacio, Instructor, College of Veterinary Medicine, University of<br />

the Philippines Los Baños<br />

C. P. Maala, Professor, College of Veterinary Medicine, University of the<br />

Philippines Los Baños<br />

The cecal mucosa of the Philippine carabao (Bubalus<br />

bubalis) was studied using transmission electron<br />

microscope. The cells lining it generally resemble those<br />

previously described in other species. The surface<br />

epithelium was lined primarily by columnar absorptive<br />

cells and a few goblet cells. The glandular epithelium was<br />

lined by goblet cells, enteroendocrine cells and

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