THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
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of the islet. The pancreatic- polypeptic- immunoreactive<br />
cells were commonly observed at the dense peripheral<br />
region of the islet. Of the four cell types localized, the least<br />
common were somatostatin- immunoreactive cells that<br />
were usually found at the peripheral region of the islet. The<br />
four cell types were of various shapes. Isolated cells of<br />
each cell type were found inside some of the pancreatic<br />
acini. Pancreatic- polypeptide- immunoreactive cells<br />
occurring singly were also found in some duct epithelium.<br />
The findings of this study suggest that the anatomy of the<br />
endocrine pancreas of the Philippine carabao is similar to<br />
that of cattle.<br />
Keywords: carabao. histological and immunological<br />
observations, islet of Langerhans, pancreas, polypeptideimmunoreactive<br />
cells.<br />
*Abstracts of Completed Researches, 1981-1995, (1996),<br />
PCC-DA, UPLB. Also in OED-PCC-DA Abstracts: Water<br />
Buffalo Researches in the Philippines, 1981-1995. p. 35.<br />
Part of the thesis submitted by the senior author for his MS<br />
degree in Veterinary Medicine.<br />
A-6<br />
IMMUNOHISTOCHEMICAL DEMONSTRATION OF<br />
FOUR CELL TYPES IN <strong>THE</strong> ENDOCRINE PANCREAS<br />
OF <strong>PHILIPPINE</strong> CARABAO, (Bubalus bubalis)*<br />
E. T. Baltazar, Assistant Professor, College of Veterinary Medicine, Central<br />
Mindanao University, Musuan, Bukidnon<br />
C. P. Maala, Associate Professor, College of Veterinary Medicine, University<br />
of the Philippines Los Baños<br />
V. G. Momongan, Professor, Institute of Animal Science, College of<br />
Agriculture, University of the Philippines Los Baños<br />
N. Kitamura, Professor, Obihiro University of Agriculture and Veterinary<br />
Medicine, Obihiro, Japan<br />
Tissue samples used in the study were collected from<br />
freshly slaughtered, apparently healthy adult Philippine<br />
carabaos of both sexes. Insulin-, glucagon-, somatostatinand<br />
bovine pancreatic polypeptide- immunoreactive cells<br />
were observed by light microscopic immunocytochemistry<br />
using Avidin Biotin Peroxidase Complex Method. The<br />
insulin cells were concentrated at the central portion of the<br />
islets while the glucagons, somatostatin and the bovine<br />
pancreatic polypeptide cells were observed mainly at the<br />
periphery. Isolated cells of each cell type were observed<br />
among the acini.<br />
Keywords: endocrine pancreas, carabao,<br />
immunocytochemistry, Bubalus bubalis<br />
*Proceedings of the 8 th Congress of the Federation of<br />
Asian Veterinary Associations, Manila, Philippines (1992);<br />
pp. 416-419. Part of the thesis submitted by the senior<br />
author for his MS degree in Veterinary Medicine.<br />
ANATOMY<br />
A-7<br />
IMMUNOHISTOCHEMICAL STUDY OF ENDOCRINE<br />
CELLS IN <strong>THE</strong> GASTROINTESTINAL TRACT OF<br />
<strong>THE</strong> <strong>PHILIPPINE</strong> CARABAO (Bubalus bubalis)*<br />
E. T. Baltazar, Assistant Professor, College of Veterinary Medicine, Central<br />
Mindanao University, Musuan, Bukidnon<br />
N. Kitamura, Associate Professor, Obihiro University of Agriculture and<br />
Veterinary Medicine, Obihiro, Japan<br />
E. Hondo, Assistant Professor, Obihiro University of Agriculture and<br />
Veterinary Medicine, Obihiro, Japan<br />
J. Yamada, Professor, Obihiro University of Agriculture and Veterinary<br />
Medicine, Obihiro, Japan<br />
C. P. Maala, Professor, College of Veterinary Medicine, University of the<br />
Philippines Los Baños<br />
L. T. Simborio, Assistant Professor, College of Veterinary Medicine,<br />
Central Mindanao University, Musuan, Bukidnon<br />
The distribution and frequency of occurrence of endocrine<br />
cells in the gastrointestinal tract of the Philippine carabao<br />
(Bubalus bubalis) were studied by immunohistochemistry.<br />
Fourteen types of immunoreactive (IR) endocrine cells<br />
were revealed. Among the cell types, only chromogranin,<br />
serotonin, and bovine pancreatic polypeptide (BPP) were<br />
present in the entire gut, while the others showed restricted<br />
distribution: somatostatin, gastrin, and cholecystokinin in<br />
the abomasum and small intestine; methionine- enkephaline-<br />
Arg 6 -Gly 7 -Leu 8 , motilin, neurotensin, secretin, gastric<br />
inhibitory peptide, and substance P in the small intestine;<br />
peptide tyrosine tyrosine (PYY) in the large intestine; and<br />
glucagons in the whole intestinal tract. Most of the cell<br />
types showed peak density in the pyloric, duodenal, or<br />
rectal region. The highest cell type heterogeneity was<br />
observed in the duodenum. The distribution profile of the<br />
gut endocrine cells in the carabao is closely related to that<br />
in the Holstein cattle. Important findings include the<br />
occurrence of BPP-IR cells in the entire gut and the high<br />
frequency of PYY-IR cells in the large intestine.<br />
Keywords: immunohistochemistry, endocrine cells,<br />
gastrointestinal tract, Philippine carabao, Bubalus bubalis<br />
*Anatomia Histologia Embryologia, (27): 407-411, 1998.<br />
A-8<br />
A STUDY ON <strong>THE</strong> LOCATION OF TASTE BUDS IN<br />
<strong>THE</strong> TONGUE OF <strong>THE</strong> <strong>PHILIPPINE</strong> SWAMP<br />
<strong>BUFFALO</strong> (Bubalus bubalis L.)*<br />
D. G. V. Boydon, Student, College of Veterinary Science and Medicine,<br />
Central Luzon State University, Muñoz, Nueva Ecija<br />
Taste buds were examined in hematoxylin eosin stained<br />
tissue sections using a light microscope. Taste buds were<br />
found only on the lateral surface of the circumvallate papillae<br />
3