THE PHILIPPINE WATER BUFFALO

ANATOMY
A-1
DISTRIBUTION AND MICROSCOPIC STRUCTURE
OF THE LINGUAL PAPILLAE OF THE PHILIPPINE
SWAMP BUFFALO (Bubalus bubalis)*
M. R. Acosta, Student
A total of thirty tongues from the Philippine swamp buffalo
taken from slaughterhouses were used in this study. Five
types of lingual papillae were observed namely: flavor,
fungiform, conical, lenticular and circumvallate papillae.
There were no foliate papillae seen. The filiform papillae
were the most numerous lingual papillae covering the entire
dorsal surface except at the region of the torus linguae.
Irregularly distributed along these papillae are the fungiform
papillae. The latter are concentrated on the tip, lateral edges
and periphery of the ventral surface of the tongue. On the
dorso-lateral surface of the tongue at the level of its root
were the circumvallate papillae arranged in V-shape pattern
with the apex pointing caudally. At the periphery of the
torus linguae were the conical papillae, the largest papillae
observed. While at the middle of the torus linguae were the
lenticular papillae appearing as round convex structures.
Microscopically, the layers comprising the epithelium of all
the papillae were the same as that of the skin. Differences
were observed on the thickness of each layer in each
papillae; the lenticular and filiform papillae showed a thick
stratum corneum while the other three with thin stratum
corneum. The long connective tissue papillae and epithelial
pegs in the lenticular and filiform papillae suggest a stronger
attachment of these papillae to the underlying tissues than
that of vallate, fungiform and conical papillae with shorter
epithelial pegs and connective tissue papillae.
Keywords: microscopic structure, lingual papillae,
Philippine swamp buffalo, Bubalus bubalis
* Undergraduate thesis, College of Veterinary Science
and Medicine, Central Luzon State University, Muñoz,
Nueva Ecija, 1988.
A-2
HISTOCHEMISTRY OF THE HYPOPHYSIS CEREBRI
OF THE PHILIPPINE CARABAO (Bubalus bubalis)*
L. G. Advincula, Student
This study was conducted to describe the different cell
types of the pituitary gland or hypophysis cerebri based on
their histochemical reaction and to identify their locations.
ANATOMY
Five hypophysis cerebri from mature male and female
Philippine carabao were collected. Tissue samples were
fixed in the 10% neutral buffered formalin and subjected to
routine paraffin technique. Tissue sections were stained
with Period Acid Schiff (PAS), Heidenhain’s Azan Mallory
stain (HAMS) and Performic Acid Alcian Blue-Periodic
Acid Schiff-Orange G (FPAAB-PAS-OG) to demonstrate
the chemical constituents of the cells of the pituitary gland.
Five cell types were demonstrated. The chromophobes
were grayish pink with HAMs. The acidophils were
differentiated into yellow and red with HAMs and the
basophils were blue with PAS and FPAAB-PAS-OG and
purple with PAS or violet with FPAAB-PAS-OG. Through
their color reaction, their location in the pars distalis were
identified. The chromophobes were scattered throughout
the pars distalis. The yellow acidophils were more
concentrated in the dorso-lateral margin of the acidophilic
zone while the carmine acidophils were more in the cranioventral
margin, through both of them occur in the entire
parenchyma of the pars distalis. The blue basophils were
located more in the lateral margin of the caudal basophilic
zone while the purple or violet basophils were
more in the caudo-dorsal margin. Among the different types
of cells identified in the pars distalis, the acidophils
predominated both in the male and female followed by
basophils and chromophobes as the least cells.
Keywords: histochemistry, pituitary gland, (hypophysis
cerebri), Philippine carabao, Bubalus bubalis
*Undergraduate Thesis, College of Veterinary Science
and Medicine, Central Luzon State University, Muñoz,
Nueva Ecija, 1998.
A-3
IMMUNOHISTOCHEMICAL STUDY OF ENDOCRINE
CELLS IN THE PANCREAS OF PHILIPPINE
CARABAO, Bubalus bubalis L.*
E. T. Baltazar, Assistant Professor, College of Veterinary Medicine, Central
Mindanao University, Musuan, Bukidnon
C. P. Maala, Professor, College of Veterinary Medicine, University of the
Philippines Los Baños
N. Kitamura, Associate Professor, Obihiro University of Agriculture and
Veterinary Medicine, Obihiro, Japan
E. Hondo , , Assistant Professor, Obihiro University of Agriculture and
Veterinary Medicine, Obihiro, Japan
G. D. de Ocampo, Professor, College of Veterinary Medicine, University
of the Philippines Los Baños
E. F. Landicho, Associate Professor, College of Veterinary Medicine,
University of the Philippines Los Baños
J. Yamada, Professor, Obihiro University of Agriculture and Veterinary
Medicine, Obihiro, Japan
The occurrence and frequency of endocrine cells in the
pancreas of the Philippine carabao (Bubalus bubalis L.)
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