THE PHILIPPINE WATER BUFFALO

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vaccination given at Day 28. Antibody titer for all types, however, were below the protective levels having a logarithmic mean for all types of 1.9617, 4.2938, 3.1099, 3.2295, 1.5907 at Day 21, 60, 90, 120, and 150 respectively. Statistical analysis indicates a significant difference between the oil and aqueous vaccines. Swellings at the site of inoculation were observed for both types of vaccine however, the swelling subsided after a week of observation. Keywords: FMD vaccine, oil-adjuvanted, aqueous, water buffalo *Undergraduate thesis, College of Veterinary Medicine, University of the Philippines Los Baños, 1999. H-24 SKIN LESIONS OF PHILIPPINE CARABAOS* E. A. Macarandang, Student Various skin lesions were observed in 250 carabaos at slaughter. Representative samples of skin lesions in 49 animals were grouped as follows: 1) transverse cracks and crevices with moth-eaten appearance of the hoof attributable to Booponus intonsus; 2) tumor-like masses with slight inflammatory changes on microscopic examination; 3) hyperkeratinized, depigmented, hairless areas with acute and chronic inflammatory lesions; 4) areas of encrustations with folliculitis and parasitism, and 5) hemorrhagic nodules which on histological examination revealed nematodes and microfilariae. Keywords: skin lesions, inflammation, tumor, carabao *Undergraduate thesis, College of Veterinary Medicine, University of the Philippines Diliman, 1971. H-25 SEROLOGICAL SURVEY OF BRUCELLOSIS IN CATTLE AND CARABAO SLAUGHTERED AT A BACOLOD ABATTOIR USING ROSE BENGAL AGGLUTINATION TEST * N. M. Malvas, Student A serological survey of 110 cattle and 167 carabao slaughtered in a Bacolod City abattoir in October 1989, indicated that 2.7% of the cattle population tested had evidence of brucella infection. The presence of even a small number of positive reactors in the cattle population was significant because it was indicative of possible Brucella abortus infection in the herd of origin. There was no positive reactor in the carabao serum samples tested. 138 ABSTRACT OF RESEARCHES ON THE PHILIPPINE WATER BUFFALO Since RBAT was less sensitive in testing buffalo serum, an assay of higher sensitivity should be utilized. With the initial finding of positive reactor, in cattle slaughtered in the Bacolod abattoir, a more exhaustive investigation in the area of possible reactors is imperative. Keywords: brucellosis, Rose Bengal agglutination test, cattle, carabao *Undergraduate thesis, College of Veterinary Medicine, University of the Philippines Los Baños, 1990. H-26 A STUDY ON THE OCCURRENCE OF TUBERCULOSIS AMONG DAIRY BUFFALOES IN STA.MARIA BULACAN AND CENTRAL LUZON STATE UNIVERSITY USING OLD TUBERCULIN AND KAOLIN AGGLUTINATION TEST* B. P. Mateo, Student A study on the occurrence of tuberculosis among dairy buffaloes of Sta. Maria, Bulacan and Central Luzon State University using old tuberculin (OT) and Kaolin Agglutination (KA) test is herein presented. A total of one hundred buffaloes were tested. Fourteen animals were diagnosed as tuberculous and six animals were suspected of the disease. Kaolin agglutination test shows a good correlation with the results of tuberculin testing. All positive tuberculin reactors showed marked increase in serum titer collected two weeks after tuberculin testing. It may be used in conjunction with tuberculin testing to improve the accuracy of testing in an eradication program of tuberculosis. Keywords: tuberculosis, old tuberculin test, kaolin agglutination test, dairy buffalo *Undergraduate thesis, College of Veterinary Medicine, University of the Philippines Los Baños, 1978. H-27 ISOLATION AND SEROTYPING OF Pasteurella multocida IN CATTLE (Bos indicus) AND CARABAO (Bubalus bubalis)* A. S. Ng, Student A total of 220 samples collected from cattle and carabao using different methods of isolation yielded Pasteurella multocida isolates. These were NS 1 serotype as type B, PBS 11 as type B, and pBS 17 as type D. Hemagglutination, indirect hemagglutination, agar gel diffusion, and Acriflavin tests were used in the identification of capsule serotype. Pasteurella multocida organism can be isolated from the
upper respiratory tract of normal cattle. They were present in the lower respiratory tract when the animal succumbed to a more chronic infection. Lung sampling and nasal swabs were good methods for isolating the organism from the animals. The present study showed a carrier rate of 1.36% in the normal cattle observed, which lower than previously reported (3.5%) in cattle. Keywords: Pasteurella multocida, carabao, carrier rate, cattle, respiratory infection *Undergraduate thesis, College of Veterinary Medicine, University of the Philippines Diliman, 1988. H-28 PREVALENCE SURVEY OF TUBERCULOSIS IN CARABAOS (Bubalus bubalis) IN FOUR MUNICIPALITIES OF CAMARINES SUR BY INTRADERMAL TEST WITH HUMAN PURIFIED PROTEIN DERIVATIVE* R. M. Olaso, Student A study of the prevalence of tuberculosis in native carabaos (Bubalus bubalis) by intradermal test with human purified protein derivative (PPD) was conducted. A total of 248 carabaos in the four municipalities of Camarines Sur were tested using human PPD by intradermal test. This figure comprises about 4.8% of the total Carabao population in the said municipalities. The result of the survey yielded negative results, despite the high incidence of human tuberculosis in the said area. Likewise, the Stormont tests were negative. The findings in the survey, therefore, did not seem to support the hypothesis of transmission of human tuberculosis to Carabao. As shown in this survey, a zero prevalence of tuberculosis in carabaos was found using PPD of human origin. Tuberculosis in carabao was very rare, probably because the animal is resistant to infection with mycobacterium tuberculosis as claimed by some workers. Keywords: tuberculosis, intradermal test, carabao *Undergraduate thesis, College of Veterinary Medicine, University of the Philippines Diliman, 1982 H-29 FIELD TRIAL OF Pasteurella multocida TYPE B VACCINE IN PURE AND GRADED PHILIPPINE CARABAOS* C. M. Quebec Jr., Student Crude capsular antigen (CCA) of Pasteurella multocida type B vaccines was tested in 30 carabaos in Casuntingan HEALTH and Causwagan, Macarthur, Leyte, and the antibody titer was monitored using IHA test. No significant differences were observed between the immune response of carabaos in Casuntingan and of those in Causwagan, and also between IM or SC route of vaccine administration. The vaccine therefore, can be administered either subcutaneously or intramuscularly. Mouse protection test of immune serum from vaccinated carabaos conferred 87% survival in the experimental group challenged with 100LD 50 whereas the control groups had 100% mortality. The present study showed that CCA vaccine of P. multocida was able to elicit protective antibodies up to 210 days post vaccination therefore the vaccine has a very good potential to offer protection in carabaos against hemorrhagic septicemia. Keywords: Pasteurella multocida, vaccine, capsular antigen, hemorrhagic septicemia, carabao *Undergraduate thesis, College of Veterinary Medicine, University of the Philippines Los Baños, 1990. H-30 COMPARATIVE EFFICACY OF TOBACCO (Nicotiana tobaccum), MAKABUHAY (Tinosphora rumphi), TUBLI (Derris philippinensis) AND NEEM (Azadirachta indica) AGAINST CARABAO LOUSE (Haematopinus tuberculatus)* A. P. Robles, Student The efficacy of tobacco (Nicotiana tobaccum), tubli (Derris philippinensis), makabuhay (Tinosphora rumphi) and neem (Azadirachta indica) in oil-immersion and mixture in distilled water at 10%, 20% and 40% concentrations were evaluated for effectiveness against carabao louse (Haematopinus tuberculatus) in vitro. Four hundred five (405) mature H. tuberculatus were used in the study. In vitro experimentation showed that mixtures in oil emulsion had a percentage mortality of 90 or better. Mixtures in distilled water showed that only tobacco obtained a percent mortality greater than 80 in all concentrations. Tubli was most effective at 40% concentration with mean percent mortality of 24 after 5 hours of exposure. Neem and makabuhay were least effective with mean percentage mortality of less than 10 in all concentrations. Positive control using pure mineral oil obtained a percentage mortality of 86 while positive control using distilled water showed no effect. Further test in vivo showed that the lowest concentration in both preparations which yielded a percent mortality of 80 or better after 5 hours of exposure in vitro was used in in vivo. Thirty-two (32) infested carabaos from the Philippine Carabao Center (PCC), UPLB were used. 139
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ABSTRACT OF RESEARCHES ON THE PHILI
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USER’S GUIDE The book contains pu
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ACKNOWLEDGMENT Grateful recognition
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CONTENTS Inoc, J. G. and C. P. Maal
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CONTENTS Viernes, Jr., V. D. 2004.
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CONTENTS Harada, H., et al. Ultraso
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CONTENTS Ocampo, M. B., et al. Clea
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CONTENTS Aranas, T. J., et al. 1968
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CONTENTS Romulo, B. H., et al. Dige
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ANATOMY A-1 DISTRIBUTION AND MICROS
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of the islet. The pancreatic- polyp
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(P>0.05) from that of the Philippin
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A-15 THE MODIFIED HORN RING METHOD
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undifferentiated cells. Mucigenous
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*Abstracts of Completed Researches,
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A-31 SCANNING ELECTRON MICROSCOPIC
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cells” had fewer zymogen granules
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is recommended that two years old P
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Their secretions, together with tho
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elated peptide (CGRP), vasoative in
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methods. The diameter of the semini
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appearance to the fungiform papilla
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of pyknotic nuclei in the follicula
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observed. Continuous medullary patt
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examination of the muzzle prints no
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conspicuously absent in the tunica
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esembles closely that of the lympho
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obtained for total serum protein, a
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A study in the conception rate of t
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significantly (P
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and Philippine-Murrah in health. Ei
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months to 3 years. Erythrocyte size
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were highly significant level (P
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THE CATTLE AND CARABAO BREEDING PRO
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estimated breeding values of (EBVs)
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and broken horn were common among t
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the Alb A Alb B and Alb B Alb B gen
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different in trms of shear force va
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stock farms which practiced backyar
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Ravi was at the top. The heterosis
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& 4):61-66. Also in Annotated Bibli
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Series of experiment were conducted
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Keywords: embryo, in vitro fertiliz
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The percentages of pregnant cattle
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after the second injection. The con
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INSEMINATION OF PHILIPPINE CARABAO
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It was noted that regardless of the
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the presence or absence of cysteami
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R. de Vera, Laboratory Aid, PCC-OED
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on the factors affecting embryo rec
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Expanded blastocysts did nor surviv
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Early pregnancy diagnosis was condu
Page 105 and 106: ng/ml and dropped sharply at partur
Page 107 and 108: R-67 MANAGEMENT AND CLIMATIC FACTOR
Page 109 and 110: Keywords: carabao heifers, estrous
Page 111 and 112: animal draft power was designed and
Page 113 and 114: Water Buffalo Researches in the Phi
Page 115 and 116: D-13 SOME PHYSIOLOGICAL AND BIOCHEM
Page 117 and 118: FEEDING AND NUTRITION F-1 OBSERVATI
Page 119 and 120: W. L. Johnson, Former Graduate Assi
Page 121 and 122: in a majority of the carcass compon
Page 123 and 124: F-16 FEEDING VALUE OF CORN STOVER-M
Page 125 and 126: (DDMI) of rice straw per 100 kg bod
Page 127 and 128: D. D. Lorenzo, Instructor, Departme
Page 129 and 130: Tethering, soiling, communal pastur
Page 131 and 132: C. R. Legaspino, Former Student, De
Page 133 and 134: 70.97 mg percent. Age and sex seeme
Page 135 and 136: levels (8.3 mg/100 ml vs. 11.0 mg/1
Page 137 and 138: for the first 7 months. Tethering p
Page 139 and 140: L. S. Castillo, Professor, Departme
Page 141 and 142: Keywords: caraheifers, chromic oxid
Page 143 and 144: similar. Addition of 1% urea in the
Page 145 and 146: espectively. Detergent fiber analys
Page 147 and 148: V. S. Subere, Former M. S. Student,
Page 149 and 150: serovars were used namely: Leptospi
Page 151 and 152: was conducted in the abattoirs of M
Page 153 and 154: Pseudomonas sp. (2.86%), Pasteurell
Page 155: *Undergraduate thesis, College of V
Page 159 and 160: studies are necessary to find possi
Page 161 and 162: permanent cheek teeth, 26 permanent
Page 163 and 164: uy back operation, 11 calving inter
Page 165 and 166: caraheifers, 2, 27, 45 carcass qual
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THE PHILIPPINE WATER BUFFALO

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