THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
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Keywords: carabao heifers, estrous cycle, estrus<br />
ovulation, gestation<br />
*Abstracts of Completed Researches 1981-1995.<br />
(1981). PCC-UPLB, College, Laguna. Also in Abstracts<br />
of Water Buffalo Researches in the Philippines 1981-<br />
1995. 1996. PCC-DA. p. 78. Also in Annotated Bibliography<br />
on Philippine Biodiversity: Livestock and Poultry<br />
(Agrobiodiversity) 1949-1997, Carabaos. 2002. p. 299.<br />
R-73<br />
<strong>THE</strong> EFFECTS OF PROSTAGLANDIN F 2 ALPHA<br />
(LUTALYSE) ON ESTRUS, OVULATION AND<br />
PREGNANCY IN <strong>THE</strong> <strong>PHILIPPINE</strong> CARABAO*<br />
W. Wongsrikeao, Former PhD Student, IAS-CA, UPLB<br />
V. G. Momongan, Professor, IAS-CA, UPLB<br />
Thirteen normally cycling carabao cows, between 5 to 6<br />
years of age, were allocated into four treatments: group 1<br />
(n=3) received PGF 2 alpha on day 6 of the estrous cylce;<br />
group 2 (n=4) received PGF 2 alpha on day 12 of the estrous<br />
cycle; group 3 (n=3) received treatment of PGF 2 alpha on day<br />
16 th ; and group 4 (n=3) the control group did not receive any<br />
treatment of PGF 2 alpha, respectively. Every animal in<br />
treatment groups 1 to 3 was given 20.25 and 30 mg PGF 2<br />
alpha, respectively. Carabao cows receiving PGF 2 alpha<br />
treatment on day 6 of the estrous cycle failed to respond to<br />
PGF 2 alpha injection. However, carabao cows given PGF 2<br />
alpha on day 12 or 16 of the estrous cycle showed<br />
synchronized estrus. The length of the subsequent estrous<br />
cycle, estrus duration and ovulation time of PGF 2 alpha treated<br />
and control carabao cows were not significantly different.<br />
Keywords: carabao cows, estrous cycle, estrus<br />
ovulation, estrus synchronization, prostaglandin F 2 alpha<br />
*Abstracts of Completed Researches 1981-1995.<br />
(1981).PCC-DA, UPLB, College, Laguna. Also in Abstracts<br />
of Water Buffalo Researches in the Philippines<br />
1981-1995.1996.PCC-DA. p.79. Also in Annotated Bibliography<br />
on Philippine Biodiversity: Livestock and Poultry<br />
(Agrobiodiversity) 1949-1997, Carabaos. 2002. p. 387.<br />
R-74<br />
BLOOD GONADOTROPIC ACTIVITY DURING <strong>THE</strong><br />
ESTROUS CYCLE OF CARABAO HEIFERS*<br />
W. Wongsrikeao, Graduate Student, DAS-CA, UPLB<br />
V. G. Momongan, Associate Professor, DAS-CA, UPLB<br />
T. A. Abilay, Assistant Professor, DAS-CA, UPLB<br />
The estrous cycle of the female is a product of a highly<br />
integrated relationship between the ovaries and<br />
adenohypophysis. Studies on gonadotropins in this animal<br />
under Philippine condition, however, has not been done. A<br />
study was conducted to assess the blood gonadotropic<br />
REPRODUCTION<br />
activity of three-year old normally cycling carabao heifers<br />
relative to the stages of estrous cycle. A bioassay technique<br />
was used in the determination of blood gonadotropic activity.<br />
The test parameters involved the monitoring of the increase<br />
in testicular size and seminiferous tubule development of a<br />
day old male chicks. The results indicated that blood<br />
gonadotropic activity of carabao heifers was lowest at<br />
estrus, increased to a maximum on day 12 and declined<br />
after day 15, reaching a low value on day 20.<br />
Keywords: blood gonadotropin activity, bioassay<br />
technique, cycling carabao heifers, estrous cycle.<br />
*Phil. J. Vet. Anim. Sci. 1979. 5(1):65-70. Also in Annotated<br />
Bibliography on Philippine Biodiversity:Livestock and Poultry<br />
(Agrobiodiversity) 1949-1997, Carabaos. 2002. p. 320<br />
R-75<br />
SYNCHRONIZATION OF ESTRUS WITH PGF2 ALPHA (LUTALYSE) AND FERTILITY IN <strong>PHILIPPINE</strong><br />
CARABAOS*<br />
W. Wongsrikeao, Former PhD Student, IAS-CA, UPLB<br />
T. A. Abilay, Consultant, MADECOR<br />
V. G. Momongan, Professor, IAS-CA, UPLB<br />
T. Blanchard, Former Visiting Professor, IAS-CA, UPLB<br />
A. R. Obsioma, Research Assistant, IAS-CA, UPLB<br />
Two experiments were conducted to assess the effect of<br />
PGF 2 alpha on the estrus induction and fertility in the Philippine<br />
carabaos. In experiment 1, twelve normally cycling carabao<br />
cows, 6 to 6.5 years of age are randomly distributed into two<br />
groups: no prostaglandin (PGF 2 ) alpha treatment (control)<br />
and 30 ng PGF 2 alpha intramuscular injection on day 12 of<br />
the estrous cycle. In experiment 2, nine animals were<br />
randomly assigned to two groups: no PGF 2 alpha treatment<br />
(control, n=5) and 30 ng PGF 2 alpha intramuscular injection<br />
12 days apart (n=4) to those carabaos which failed to manifest<br />
estrus after the first injection. All experimental animals were<br />
artificially inseminated twice at 12 and 20 hours after onset of<br />
estrus. The result of this study indicated that estrus and<br />
ovulation could be synchronized in carabaos. The pregnancy<br />
rates of animals treated intramuscularly with a single injection<br />
of 30 ng PGF 2 alpha on day 12 of the estrous cycle or two<br />
injections of 30 ng PGF 2 alpha, 12 days apart are comparable<br />
to those obtained in the control animals.<br />
Keywords: carabaos, estrus synchronization, ovulation,<br />
prostaglandin F 2 alpha<br />
*Abstract of Completed Researches 1981-1995. (1981).<br />
PCC-UPLB, College, Laguna. Also in Abstracts of Water<br />
Buffalo Researches in the Philippines 1981-1995. 1996.<br />
PCC-DA. p. 79. Also in Annotated Bibliography on<br />
Philippine Biodiversity: Livestock and Poultry<br />
(Agrobiodiversity) 1949-1997, Carabaos. 2002. p. 322.<br />
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