THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
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R-67<br />
MANAGEMENT AND CLIMATIC FACTORS<br />
AFFECTING LIBIDO, SEXUAL BEHAVIOR AND<br />
SEMEN QUALITY OF <strong>BUFFALO</strong> BULLS*<br />
H. V. Venturina, Science Research Specialist, PCRDC-PCARRD, CLSU<br />
F. V. Mamuad, Assistant Professor, DAS-CA, CLSU<br />
P. G. Duran, Science Research specialist, PCRDC-PCARRD, CLSU<br />
F. P. Aquino, Science Research Assistant, PCRDC-PCARRD, CLSU<br />
M. F. Pineda, Science Research Aide, PCRDC-PCARRD, CLSU<br />
N. P. Garcia, Science Research Assistant, PCRDC-PCARRD, CLSU<br />
R. L. Osio, Science Research Specialist, PCRDC-PCARRD, CLSU<br />
D. T. Esteban, Laboratory Aide, PCRDC-PCARRD, CLSU<br />
Twelve bulls, six Murrah buffaloes (MB), four Philippine<br />
carabaos (PC), and two crossbreds (CB) with ages ranging<br />
from 3 to 5 years were used in this study. A total of 1911<br />
collection attempts were conducted. From this, 1586<br />
successful attempts were observed (82.99%) and 829<br />
ejaculates (52.27%) were evaluated for volume, 749<br />
(47.23%) for motility, and 729 (45.96%) for sperm<br />
concentration.<br />
Keywords: buffalo bulls, semen quality<br />
*Phil. J. Vet. Anim. Sci. 1986. 12 (3&4):72-73 (Abstr.).<br />
Also in Abstracts of Water Buffalo Researches in the<br />
Philippines 1981-1995. 1996. PCC-DA. p. 78. Also in<br />
Annotated Bibliography on Philippine Biodiversity:<br />
Livestock and Poultry (Agrobiodiversity) 1949-1997,<br />
Carabaos. 2002. p. 198.<br />
R-68<br />
OVARIAN RESPONSES OF <strong>WATER</strong> <strong>BUFFALO</strong>ES<br />
AFTER PGF 2 ALPHA INJECTION*<br />
H. V. Venturina, Science Research Specialist, PCRDC-PCARRD, CLSU<br />
F. V. Mamuad, Assistant Professor, DAS-CA, CLSU<br />
P. G. Duran, Science Research Specialist, PCRDC-PCARRD, CLSU<br />
V. G. Mauro, Instructor, CVSM, CLSU<br />
L. C. Cruz, Prof., DAS-CA, CLSU and Project Leader, PCRDC-<br />
PCARRD, CLSU<br />
Eighteen cyclic caracows and eight cyclic caraheifers<br />
were divided into two batches for observation to monitor<br />
the occurrence of estrus, follicular development and<br />
ovulation following prostaglandin F 2 alpha (PGF 2 alpha)<br />
treatments. AI animals received two injections of PGF 2<br />
alpha analogue (fenprostalene), 1.0 mg, and 11 days apart.<br />
Twenty-four hours after the second PGF 2 alpha injection,<br />
ovarian structures were checked per rectum at four hours<br />
interval until the occurrence of ovulation while observation<br />
of signs of estrus was made with the use of vasectomized<br />
bull. Five or 25 (19%) animals manifested signs of estrus<br />
72 hours after the second PGF 2 alpha treatment while the<br />
remaining 80% (21 of 25) had the signs of estrus noted 12-<br />
REPRODUCTION<br />
24 hours later. Ovulation occurred in 17 to 18 heifers and<br />
7 out of 8 treated cows after 91.08 hours and 87.55 hours,<br />
respectively, after PGF 2 alpha treatment. Follicular<br />
development occurred in both the left and right ovaries in<br />
14 to 17 animals. More ovulations (58.33%) occurred at<br />
the right ovary.<br />
Keywords: caracows, caraheifers, estrus, follicular<br />
development, ovulation, PGF 2 alpha treatment<br />
*Abstracts of Completed Researches, 1981-1986. 1996.<br />
PCRDC-PCARRD, CLSU, Muñoz, Nueva Ecija. Also<br />
in Abstracts of Water Buffalo Researches in the Philippines<br />
1981-1995. 1996. PCC-DA. p. 77-78. Also in Annotated<br />
Bibliography on Philippine Biodiversity: Livestock and<br />
Poultry (Agrobiodiversity) 1949-1997, Carabaos. 2002.<br />
p. 364.<br />
R-69<br />
SUPEROVULATORY RESPONSES TO PMSG AND<br />
FSH TREATMENTS IN <strong>WATER</strong> <strong>BUFFALO</strong>ES*<br />
H. V. Venturina, Science Research Specialist, PCRDC-PCARRD, CLSU<br />
O. F. Smith, PhD Student, IGS, CLSU<br />
F. A. Adraino, Science Research Analyst, PCRDC-PCARRD, CLSU<br />
P. G. Duran, Science Research Specialist, PCRDC-PCARRD, CLSU<br />
S. S. Jha, M. S. Student, IGS, CLSU<br />
T. Niwat, PhD Student, IGS, CLSU<br />
A. S. Argañosa, Deputy Director, LRD, PCARRD<br />
L. C. Cruz, Prof., DAS-CA, CLSU and Project Leader, PCRDC-PCARRD,<br />
CLSU<br />
Eight cyclic buffaloes were used to determine suitable time<br />
for superovulation. Group A received pregnant mare serum<br />
gonadotropin (PMSG) (3000 i,u,) on day 9 while group B<br />
treatment, on day 15 of the cycle, prostaglandin F 2 alpha<br />
(PGF 2 alpha) were given 60 hours and 72 hours after<br />
PMSG and ovaries were obtained two days after estrus to<br />
monitor ovarian responses. The last study compared the<br />
superovulatory responses of 14 buffaloes to either PMSG<br />
(3000 i.u.) or follicle stimulating hormone, porcine (pFSH)<br />
(44 mg, decreasing dose of 7.7, 6.5, 5.5, 4.4 mg at 12<br />
hours interval). Treatments were initiated on day 9-10,<br />
PGF 2 alpha on 60 and 72 hours after gonadotropin. AI on<br />
12 and 24 hours after estrus and embryos were collected<br />
on day 5.5 after breeding. Superovulatory treatments<br />
initiated either on day 9 or day 15 were similar with regards<br />
to number of ovulations and number of growing follicles.<br />
The pFSH treatments resulted to more ovulations (2.86 +<br />
1.57) and collected embryos (2.0 + 2.0) than PMSG (1.5<br />
+ 2.0).<br />
Keywords: cyclic buffaloes, porcine follicle stimulating<br />
hormone, pregnant mare serum gonadotropin, PGF 2 alpha,<br />
superovulatory responses<br />
89
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