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THE PHILIPPINE WATER BUFFALO

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H. V. Venturina, Science Research Specialist, PCRDC-PCARRD, CLSU<br />

F. A. Adriano, Science Research Analyst, PCRDC-PCARRD, CLSU<br />

P. Serra, Science Aide, PCRDC-PCARRD, CLSU<br />

L. C. Cruz, Prof., DAS-DA, CLSU and Project Leader, PCRDC-PCARRD,<br />

CLSU<br />

Six mature and healthy Philippine carabaos (PC) were<br />

selected after having shown normal estrus cycles for three<br />

consecutive times. All animals were fed in confinement<br />

and were fasted for at least 6 hours prior to actual ovarian<br />

observations. A simple fiberscope (FS-100, Scott Fiber<br />

Optics, Inc., MA USA) was inserted through a puncture<br />

on the vaginal floor following administration of anesthetics<br />

at the sacro-lumbar region. Observations of ovarian<br />

structures were done every 3 days throughout the estrous<br />

cycle using the same route. Using the simple fiberscope,<br />

surface follicles 3.0 mm and corpus luteum (CL) were<br />

observed. There was no alteration in the duration of the<br />

length of the estrous cycle associated with repeated<br />

observations. The procedure allowed observation of<br />

follicular changes throughout the cycle and permitted direct<br />

observation of changes in the ovary related to ovulation.<br />

Keywords: fiberscope, ovarian structures, Philippine<br />

caracows<br />

*Proc. 3 rd World Buffalo Congress, Bulgaria. 1991. 1:108.<br />

Also in Abstracts of Water Buffalo Researches in the<br />

Philippines 1981-1995. 1996. PCC-DA. p. 74-75. Also<br />

in Phil. J. Vet. 1990. 16(3&4):58-59 (Abstr.). Also in<br />

Annotated Bibliography on Philippine Biodiversity:<br />

Livestock and Poultry (Agrobiodiversity) 1949-1997,<br />

Carabaos. 2002. p. 345.<br />

R-65<br />

VERIFICATION OF FARMERS’ PERCEPTION OF<br />

OCCURRENCE OF ESTRUS (HEAT) CARABAOS<br />

DURING <strong>THE</strong> FULL PHASE OF <strong>THE</strong> MOON*<br />

A. A. Taveros, Instructor, DASVM, ViSCA<br />

A. M. Taveros, Assistant Professor,, DASVM, ViSCA<br />

O. B. Posas, Professor, DASVM, ViSCA<br />

Bimonthly visits were made to different barangays of<br />

Baybay, Albuera and Inopacan, Leyte. Caracows, which<br />

were naturally in heat were inseminated while the others<br />

were artificially induced using prostaglandin F 2 alpha and<br />

subsequently inseminated. It was observed that during<br />

the full phase of the moon, 85.90% of the caracows were<br />

naturally in heat while all (100%) of the artificially induced<br />

animals came into heat after injection with the hormone.<br />

Keywords: caracows, farmers’ perception of occurrence<br />

of estrus<br />

*Ann. Trop. Res. 1996. 15:42-47 (ViSCA). Also in<br />

88<br />

ABSTRACT OF RESEARCHES ON<br />

<strong>THE</strong> <strong>PHILIPPINE</strong> <strong>WATER</strong> <strong>BUFFALO</strong><br />

Abstracts of Water Buffaloe Researches in the Philippines<br />

1981-1995. PCC-DA. 1996. p. 76. Also in Annotated<br />

Bibliography on Philippine Biodiversity: Livestock and<br />

Poultry (Agrobiodiversity) 1949-1997, Carabaos. 2002.<br />

p. 358.<br />

R-66<br />

SUCCESSFUL PREGNANCY OF PUREBRED<br />

MURRAH <strong>BUFFALO</strong> EMBRYO ON <strong>PHILIPPINE</strong><br />

CARABAO RECIPIENT*<br />

H. V. Venturina, Science Research Specialist, PCRDC-PCARRD, CLSU<br />

S. S. Jha, M.S. Student, IGS, CLSU<br />

F. Adriano, Science Research Analyst, PCRDC-PCARRD, CLSU<br />

P. Duran, Science Research Specialist, PCRDC-PCARRD, CLSU<br />

N. Lorenzo, Science Research Aide, PCRDC-PCARRD, CLSU<br />

O. F. Smith, Ph.D. Student, IGS, CLSU<br />

P. Serra, Research Aide, PCRDC-PCARRD, CLSU<br />

L. C. Cruz, Prof., DAS-CA, CLSU. And Project Leader, PCRDC-PCARRD,<br />

CLSU<br />

Seven purebred Murrah buffalo (MB) cows with average<br />

age of 10 years and live weight were used to determine<br />

the possibility of utilizing Philippine carabao (PC) as<br />

recipients of MB embryos. All MB cows (donors) were<br />

treated with a total of 44 mg follicle stimulating hormone,<br />

porcine (pFSH) initiated on day 9 in declining dose of 7.7<br />

mg, 6.6 mg, 8.5 mg and 4.4 mg on days 9, 10, 11 and<br />

12, respectively. Serial administration of pFSH were<br />

done at 12 hours interval prostaglandin F 2 alpha (PGF 2<br />

alpha) were given to all animals 60 and 72 hours after the<br />

initial pFSH treatment. Inseminations were made 12 and<br />

24 hours after onset of estrus. All PC (recipients) were<br />

treated with PGF 2 alpha 12 hours ahead of the donor<br />

animals. Animals with good corpus luteum (CL) on day<br />

5 were selected and were used as recipient. Embryos<br />

were collected nonsurgically from donor animals on day<br />

5 ½ and were all transferred on the same day. Average<br />

number of ovulation in donor animals was 2.86 + 1.87<br />

and the average number of unovulated follicles was 2.2<br />

+ 1.79. Average recovery rate of embryo was 70%<br />

with 71.36 recovery of the flushing medium. Four of 14<br />

embryos collected from MB were of good quality and<br />

one of three embryos transferred to PC recipients resulted<br />

to successful pregnancy.<br />

Keywords: Murrah buffalo embryo, Philippine carabao,<br />

successful pregnancy<br />

*Phil. J. Vet. Anim. Sci. 1991. 17(3&4):55 (Abstr.). Also<br />

in Abstracts of Water Buffalo Researches in the Philippines<br />

1981-1995. 1996. PCC-DA. p. 78. Also in Annotated<br />

Bibliography on Philippine Biodiversity: Livestock and<br />

Poultry (Agrobiodiversity) 1949-1997, Carabaos. 2002.<br />

p. 269.

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