SERION ELISA classic Measles Virus IgG / IgM - virion\serion

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SERION ELISA classic
Measles Virus IgG / IgM
serion
The SERION ELISA classic Measles Virus IgG and IgM tests are qualitative and quantitative
immunoassays for the detection of human antibodies in serum or plasma directed against
Measles Virus. The SERION ELISA classic Measles Virus IgM test is recommended for the
determination of acute infections whereas the SERION ELISA classic Measles Virus IgG test
is used for determination of immune status and for demonstration of intrathecally synthesized
antibodies for CSF diagnostics.
Electron micrograph of a Measles Virus
(Source: Centers for Disease Control and Prevention,
United States Department of Health and
Human Services)
multianalyt TM
Pathogen
Measles Viruses occur worldwide and belong to the family
of paramyxoviridae. According to estimations by WHO one
million individuals die as a consequence of Measles Virus infections
every year. Due to the highly infectious nature of the
virus, measles is usually aquired in the childhood. The pathogen
is transmitted by close contact with infected individuals
primarily by droplet infection.
Disease
After an incubation period of 10 to 12 days Koplik´s spots
typically appear on oral mucosa in 60 to 70 % of cases. In the
course of the prodromal period flu-like symptoms may escalate
until the typical measles exanthema arises with sharply
increasing fever. The exanthema spreads from face over the
trunk to the extremities. The clinical symptoms peak rapidly
with a correspondingly rapid recovery. Complications are frequent
consisting mainly of pneumonia and encephalitis.
Infection results in a life-long immunity. A combination of
attenuated live vaccines for mumps, measles and rubella
(MMR) has been available for several years.

Diagnosis
Demonstration of IgM antibodies is commonly used as evidence
of an acute infection and produces positive test results
early on with the onset of symptoms. The demonstration of
IgG antibodies serves primarily for immune status and vaccination
control as well as for confirmation of Measles Virus
infections. The detection of IgG antibodies in CSF samples is
particularly recommended in cases of suspected encephalitis.
SERION ELISA classic Measles Virus IgG/IgM
For validation of the SERION ELISA classic Measles Virus IgG
69 serum samples with different antibody reactivities were
analyzed in an external study. 50 serum samples from healthy
blood donors were additionally tested in house. A commercially
available ELISA was used as a reference test. For
validation of the SERION ELISA classic Measles Virus IgM
86 serum samples of patients with suspected acute measles
infection were analyzed in an external study. An ELISA of a
leading European manufacturer was used as reference test.
Highlights of SERION ELISA classic Measles Virus IgG/IgM
● Use of an inactivated Measles Virus preparation (Strain Edmonston)
● Sensitive IgM detection for the diagnosis of acute infections
● Demonstration of IgG antibody activity for confirmation of Measles Virus infection,
for immune status determination and vaccination control with results expressed in mIU/ml
referenced to the international standard of the World Health Organization (WHO)
● Excellent sensitivity and specificity values
● High precision and linearity within the measurement range
● Detection of intrathecally synthesized IgG antibodies for CSF diagnostics
Order Information
SERION ELISA classic Measles Virus IgG Order Nr.: ESR 102 G
SERION ELISA classic Measles Virus IgM Order Nr.: ESR 102 M
SERION ELISA control Measles Virus IgG Order Nr.: C 102 G
SERION ELISA control Measles Virus IgM Order Nr.: C 102 M
Please visit our website www.virion-serion.com for more information on our SERION ELISA classic products.
Institut Virion\Serion GmbH
Serion Immundiagnostica GmbH
Friedrich-Bergius-Ring 19
97076 Würzburg, Germany
Sensitivity and Specificity
SERION ELISA classic Sensitivity Specificity
Measels Virus IgG > 99 % 93.3 %
Measels Virus IgM 95.4 % > 99 %
Precision
SERION ELISA classic Measles Virus IgG
Sample Mean
value
OD
Intraassay
CV (%)
(n=20)
Tel.: +49 (0)931 3045-222
Fax: +49 (0)931 3045-100
dialog@virion-serion.de
www.virion-serion.com
Mean
value
OD
Interassay
CV (%)
(n=10)
negative 0.296 4.4 0.352 9.7
positive 1.142 4.3 1.420 9.1
strong positive 2.087 2.3 2.432 7.8
SERION ELISA classic Measles Virus IgM
Sample Mean
value
OD
Intraassay
CV (%)
(n=20)
Mean
value
OD
Interassay
CV (%)
(n=10)
positive 0.631 2.2 0.600 9.4
strong positive 2.591 4.3 3.065 7.5
strong positive 2.810 3.1 3.520 8.1
Institut Virion\Serion GmbH, V3.12/10