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Cell Culture Medium

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Leibovitz L-15 <strong>Medium</strong><br />

See page 59-63 for addition of L-Glutamine<br />

and Sodium Bicarbonate<br />

<strong>Cell</strong> <strong>Culture</strong> <strong>Medium</strong><br />

Product List<br />

The Leibovitz L-15 media were formulated to promote the cell growth in medium not<br />

balanced in CO2. The formulations were developed with the sodium bicarbonate buffer.<br />

The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and<br />

galactose so they can be used under conditions of free gaseous exchange with the<br />

atmosphere. When properly supplemented, L-15 <strong>Medium</strong> supports established cell lines,<br />

such as HEp-2 and LLC-MK 2, as well as primary explants of embryonic and adult human<br />

tissue. Many viruses also have been successfully cultivated in this medium.<br />

Cat N° Description Unit / Size<br />

Liquid<br />

L0300 Leibovitz L-15 <strong>Medium</strong> w/o L-Glutamine 100 ml<br />

State : Liquid Storage : +2/+8 °C Life : 24 months 500 ml<br />

Powder<br />

P0350 Leibovitz L-15 <strong>Medium</strong> w/ L-Glutamine For 1 L<br />

State : Powder Storage : +2/+8 °C Life : 36 months For 5 L<br />

For 10 L<br />

Mc Coy's 5A<br />

Cat N° Description Unit / Size<br />

Liquid<br />

L0211 Mc Coy's 5A w/o L-Glutamine 100 ml<br />

State : Liquid Storage : +2/+8 °C Life : 24 months 500 ml<br />

L0210 Mc Coy's 5A w/ L-Glutamine 100 ml<br />

State : Liquid Storage : +2/+8 °C Life : 12 months 500 ml<br />

Powder<br />

>> See composition page 46<br />

>> See composition page 50<br />

Mc Coy's media were originally formulated for growth and support of lymphocytes. This<br />

final modification produced a medium identical to RPMI 1629. Mc Coy's 5A media support<br />

the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the<br />

propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or<br />

transformed cell types, the most current primary and continuous cell lines.<br />

P0390 Mc Coy's 5A w/ L-Glutamine w/o Sodium Bicarbonate For 1 L<br />

State : Powder Storage : +2/+8 °C Life : 36 months For 5 L<br />

For 10 L<br />

www.biowest.net<br />

29

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