Cell Culture Medium

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Cell Culture Medium Summary Cell Culture Medium . . . . . . . . . . . . . . . . . .page # Basal Medium Eagle (BME) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 CMRL 1066 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Dulbecco’s Modified Eagle’s Medium (DMEM) . . . . . . . . . . 23 - 25 DMEM High Glucose . . . . . . . . . . . . . . . . . . . . . . . . 23 - 24 DMEM Low Glucose . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 DMEM – Ham’s F12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Glasgow MEM BHK 21 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 - 26 Ham’s F10 ( F-10 Nutrient Medium) . . . . . . . . . . . . . . . . . . . . . . 26 Ham’s F12 ( F-12 Nutrient Medium) . . . . . . . . . . . . . . . . . . . . . . 27 Ham’s F14 ( F-14 Nutrient Medium) . . . . . . . . . . . . . . . . . . . . . . 28 Iscove’s Modified Dulbecco’s Medium (IMDM) . . . . . . . . . . . . . . 28 Leibovitz L15 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Mc COY’s 5A Medium modified . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Minimum Essential Medium (MEM) . . . . . . . . . . . . . . . . . . . 30 - 32 MEM w/ Earle’s Salts . . . . . . . . . . . . . . . . . . . . . . . . 30 - 31 MEM w/ Hanks’ Salts . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 MEM Alpha Modification . . . . . . . . . . . . . . . . . . . . . 31 - 32 Medium 199 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 RPMI 1640 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 - 34 Schneider’s Drosophila Medium . . . . . . . . . . . . . . . . . . . . . . . . . 35 Serum Reduced Medium - MCDB . . . . . . . . . . . . . . . . . . . . . . . . 35 Compositions . . . . . . . . . . . . . . . . . . . . . . . . . . 36 - 54 Preparation Instruction . . . . . . . . . . . . . . . . . . . . . . 55 Custom Made Formulation . . . . . . . . . . . . . . . . 56 - 59 Table of Addition of L-Glutamine . . . . . . . . . . 60 - 61 Table of Addition of Sodium Bicarbonate . . . . 62 - 64 www.biowest.net 21
22 Product List Basal Medium Eagle (BME) Cat N° Description Unit / Size Liquid L0042 BME w/ Earle's Salts w/o L-Glutamine 100 ml State : Liquid Storage : +2/+8 °C Life : 24 months 500 ml L0044 BME w/ Earle's Salts w/o L-Glutamine w/ 25 mM Hepes 100 ml State : Liquid Storage : +2/+8 °C Life : 24 months 500 ml L0043 BME w/ Earle's Salts w/ L-Glutamine 100 ml State : Liquid Storage : +2/+8 °C Life : 12 months 500 ml L0045 BME w/ Earle's Salts w/ L-Glutamine w/ 25 mM Hepes 100 ml State : Liquid Storage : +2/+8 °C Life : 12 months 500 ml L0046 BME w/ Hanks' Salts w/o L-Glutamine 100 ml State : Liquid Storage : +2/+8 °C Life : 24 months 500 ml Powder P0030 BME w/ Earle's Salts w/ L-Glutamine For 1 L w/o Sodium Bicarbonate For 5 L State : Powder Storage : +2/+8 °C Life: 48 months For 10 L P0031 BME w/ Hanks' Salts w/ L-Glutamine For 1 L w/o Sodium Bicarbonate For 5 L State : Powder Storage : +2/+8 °C Life: 48 months For 10 L Cell Culture Medium Cell Culture Medium www.biowest.net 10x concentration >> See composition page 36 Basal Medium Eagle is principaly used for diploid or primary cell cultures. It is the simplest of the basal media with all essential components for growth. BME ideally supports cell lines such as HeLa, L-cells and primary mammalian fibroblasts. X0042 BME 10X w/ Earle's Salts w/o L-Glutamine 100 ml w/o Sodium Bicarbonate 500 ml State : Liquid Storage : +2/+8 °C Life : 24 months X0026 BME 10X w/ Hanks' Salts w/o L-Glutamine 100 ml w/o Sodium Bicarbonate 500 ml State : Liquid Storage : +2/+8 °C Life : 24 months See page 59-63 for addition of L-Glutamine and Sodium Bicarbonate
Page 1 and 2: www.biowest.net orldwide ervice xce
Page 3 and 4: Company Profile 02 Company Profile
Page 5 and 6: Company Profile 04 Company Profile
Page 7 and 8: Animal Serum 06 Animal Serum GBR Le
Page 9 and 10: Animal Serum 08 Animal Serum Presen
Page 11 and 12: Animal Serum 10 Animal Serum Presen
Page 13 and 14: Animal Serum 12 Animal Serum Treatm
Page 15 and 16: Fetal Bovine Serum 14 Fetal Bovine
Page 17 and 18: Animal Serum 16 Animal Serum Produc
Page 19 and 20: Albumin Bovine 18 Albumin Bovine ww
Page 21: 20 Presentation Cell Culture Medium
Page 25 and 26: 24 Powder Product list P0102 DMEM H
Page 27 and 28: 26 Product List Powder P0120 Glasgo
Page 29 and 30: 28 Product List Ham'S F14 (F-14 Nut
Page 31 and 32: 30 Product List Minimum Essential M
Page 33 and 34: 32 Medium 199 Product List Powder P
Page 35 and 36: 34 Product List POWDER P0870 RPMI 1
Page 37 and 38: Cell Culture Medium Cell Culture Me
Page 51 and 52: 50 Product Composition Cell Culture
Page 57 and 58: 56 Custom Made Formulation For your
Page 59 and 60: 58 Custom Made Formulation VITAMINS
Page 61 and 62: 60 Table of Addition of L-Glutamine
Page 63 and 64: 62 Table of addition of Sodium Bica
Page 65 and 66: 64 Table of addition of Sodium Bica
Page 67 and 68: 66 Hanks' Balanced Salts Solution H
Page 69 and 70: Salt Solutions 68 Salt Solutions &
Page 71 and 72: Salt Solutions 70 Salt Solutions &
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Salt Solutions 72 Salt Solutions &
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Antibiotics 74 Antibiotics Product
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Antibiotics 76 Antibiotics Composit
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Cell Culture Reagents Cell Culture
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Standard Terms of Sales 88 Standard
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Albumin Bovine Serum Fraction V . .
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MEM Alpha Modification w/ Earle's S
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Cell Culture Medium

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