Cell Culture Medium

Animal Serum
Treatments
IgG low ( < 5 mg / l) :
The IgG level in the fetal bovine serum is in general between 50 and
200mg/l, it is a low level compared to the other sera as the New Born
Calf serum. However, this level can cause some problems for some utilisations,
that is why we select the sera with a level < 5mg/l for the users.
For orders please add code I
Ex : FBS origin South America : S1810
IgG low : S181I
Lipid Depletion :
Biowest uses the fumed silica precipitation method for removing lipids.
The fumed silica powder is added to the serum. It’s well mixed together.
After the solution is centrifuged. The supernatant contains the serum and
the lipids are with the silica in the pellet.
For orders please add code L
Ex : FBS origin Mexico : S1650
Lipid depleted : S165L
Calcium Stripping :
The calcium depletion is achieved using chromatography columns (ionexchange
resin). This resin is selective for divalent cations over monovalent
cations (It follows that copper, iron, magnesium, zinc etc. levels will
be lowered too) .
This serum may show some reduced growth promotion of cells requiring
the presence of calcium or other divalent cations.
For orders please add code C
Ex : FBS origin Denmark : S1750
Calcium stripped : S175C
Tetracycline :
The serum is tested for the presence of Chlortetracycline, Oxytetracycline
and Tetracycline by a liquid chromatography electrospray ionisation
tandem mass spectrometry method. The detection limit is < 0.05 mg/l.
For orders please add code T
Ex : FBS origin France : S1820
Tetracycline free : S182T
Iron Supplementation :
A 1% Ferric citrate sterile solution is added to the serum at 0,246%
(v/v). Then the serum is well mixed. Transferrin is normally 20 to 40 %
bound to iron.
For orders please add code R
Ex : FBS origin South America : S1810
Iron supplemented : S181R
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