Cell Culture Medium

www.biowest.net 

orldwide 

ervice 

xcellence 

raceability 

Biowest S.A.S. Rue de la Caille | 49340 Nuaillé | France 

Tél. : [+33] 241 464 242 | Fax : [+33] 241 463 890 | Email : biowest@biowest.net 

photographies non contractuelles ~ Photographies : O.Calvez ~ conception graphique : + d’infos www.krieitiv.com ~ Version 3 - 06/08 

Product Catalog 

Solutions for successful research

1. Company Profile . . . . . . . . . . . . . . . . . . . . . . . 2 - 4 

2 . Animal Serum . . . . . . . . . . . . . . . . . . . . . . . . 5 - 18 

Presentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 

Certificate of Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 

Treatments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12 - 13 

Fetal Bovine Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 

Animal Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 16 

Animal Plasma . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 17 

Albumin Bovine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 

3 . Cell Culture Medium . . . . . . . . . . . . . . . . . . 19 - 64 

Presentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 - 20 

Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 

Standard Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 - 35 

Composition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 - 54 

Medium Preparation Instructions . . . . . . . . . . . . . . . . . . . 55 

Medium Custom Made Formulation . . . . . . . . . . . . . 55 - 59 

Table of addition of L-Glutamine . . . . . . . . . . . . . . 60 - 61 

Table of addition of Sodium Bicarbonate . . . . . . . . 62 - 64 

4 . Salt Solutions, Salts / Buffers . . . . . . . . . . . 65 - 72 

Salt Solutions / Buffers . . . . . . . . . . . . . . . . . . . . . . 65 - 68 

Salts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69 - 70 

Composition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71 - 72 

5. Antibiotics . . . . . . . . . . . . . . . . . . . . . . . . . . 73 - 76 

6. Cell Culture Reagents . . . . . . . . . . . . . . . . . 77 - 87 

Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77 

Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 - 82 

Amino Acids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82 - 85 

Composition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86 - 87 

7. Standard Terms of Sales . . . . . . . . . . . . . . . 88 - 89 

8. Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . 90 - 92 

Summary 


01

Company Profile 

02 


Presentation 

Founded in 1987, Biowest is the European leader in the collection of animal sera, offering 

the widest range of sera and media for cell culture available on the market. Our products are 

in use throughout the academic, industrial and government research sectors in fields 

spanning cell biology, genomics, proteomics, virology, immunology, drug discovery and 

toxicology. We are certified ISO 9001 and work with a strong commitment to quality, 

reproducibility, traceability and service. 

Combined with our competitive prices and our complete range of origins, these are the 

reasons for our success. 

Vertical integration and full security 

Biowest controls the production of the sera troughout the entire process from the collections 

around the world to the final shipment of bottled serum from our wharehouse, thus 

garanteeing a vertically integreted system of production and documentation. 

Using specialized equipement and detailed SOP and audits, Biowest ensures the quality at 

every stage, thereby securing a consistently high quality with low intra-lot variation. 

Worldwide sources of sera and widest range of products 

Biowest is the ideal partner for both researchers and biopharmaceutical companies. We offer 

a wide range of geograhical origins of bovine sera representing all GBR groups defined by 

the European Food Safety Authority. 

It is important to choose the right serum origin, as different field of application require 

different specification and origins at different price levels. Biowest is your garantee of the best 

choice of serum origin and specification, adjusted to your needs. 

Our Mission : Excellence in quality and service in our fields of activity. 

Our Goals : Provide the means for successful research in cell culture. 

Pure efficiency 

Best quality and service available on the market 

Most complete range of products and services 

Biggest inventory and range of serum sources 

Commitment to adressing your special requests 

Full traceability and security due to vertical integration 

Quality system 

Biowest guarantees the best quality : We are certified ISO 9001 version 2000. 

Biowest is registered by the French Ministry of Agriculture (regulation (EC) n° 1774 / 2002) 

under the agreement n° FR 49.231.01 for the production of animal sera and plasma. 

According to the European Regulation EC n° 999/2001, female donor animals are tested for 

BSE before the corresponding fetal blood is allowed to be processed. EU is pioneer in BSE 

testing and individual identification of animals through ear tagging, which ensures the best 

possible traceability and the lowest BSE risk and makes the EU origin the first choice of 

researchers in Japan and other selective markets. 

www.biowest.net


ISO 9001 Certificate 


03


04 


EDQM Certificate 

EDQM certified FBS is your guarantee that the origin and the manufacturing process 

of the product have been certified by the European Directorate for the Quality of 

Medicines & HealthCare. The EDQM protects and promotes public and animal health 

in Europe. Its mission is to contribute to the basic human right of access to good 

quality medicines and healthcare, and to promote and protect human and animal 

health by establishing and providing official standards for the manufacture and 

quality control of medicines applicable in all the signatory states of the Convention 

for the elaboration of a European Pharmacopoeia. 

Biowest can provide Foetal Bovine serum sterile filtered and packed in his facility 

that meets the criteria described in the current version of the monograph products 

with risk of transmitting agents of animal spongiform encephalopathies no.1483 

of the European Pharmacopoeia. This is certified by the EDQM. 

The countries of origin of the serum EDQM certified are Panama, Costa Rica, Uruguay 

and Paraguay. 

www.biowest.net

Animal Serum 

Presentation 

Traceable to the Source 

When scientists select their sera, an important factor taken into consideration is 

the source. Therefore, traceability of the serum is of paramount importance. 

Biowest Fetal Bovine Serum is derived from clotted whole blood aseptically collected 

from fetus via cardiac puncture. Each manufactured batch is rigorously controlled, 

from the collection of serum and throughout all stages of its treatment and 

production through to final packaging on our premises. The product is analyzed, 

classified, and tested by Biowest before shipment to customers all over the world. 

Our Quality system can trace raw materials back to the original supplier and slaughterhouse 

where they were collected. Biowest's system of vertical integration provides 

certainty of origin and traceability of all Fetal Bovine Serum (FBS). 

Biowest Quality 

FBS “quality” is defined in relation to growth promotion characteristics of a specific 

cell line when cultured in our sera. A batch of FBS which works well for one cell line 

may not work well for another cell line. Biowest customers can purchase FBS after 

performance-testing of a sample. There are thousands of different cell lines and no 

two batches of FBS are identical ; we therefore offer a very liberal sample and reserve 

policy. 

Each batch is delivered with a Certificate of Analysis. 

Serum - Batch Reservations 

Biowest is pleased to provide free samples of different batches combined with batch 

reservations during the test period up to 60 days. The general sample size for FBS 

is 50 ml / batch. Biowest can adapt these general policy to the needs of the 

customer if requested. 

After batch testing, Biowest can hold a reservation up to 24 months for customers 

who do not have ample storage facilities. In this case, a regular shipment is planed 

to the customer. 

Technical Support 

Biowest’s experienced Technical Service Staff is available to answer questions regarding 

our quality control and all Biowest products. 

We aim to provide timely, courteous and professional service. 

Worldwide Sources 

Biowest offers a wide range of sources from countries with excellent veterinary 

status. This includes South American sources as well as European Union (EU) and 

United States Department of Agriculture (USDA) approved sources. The choice of the 

FBS source is determined by import requirements and allowed supply available for 

the different markets. 

Biowest is the ideal partner for academic researchers and biopharmaceutical companies 

who select FBS based on origin and performance. 


05

Animal Serum 

06 

Animal Serum 

GBR 

Level 

Presentation 

Risk Classification GBR 

Serum producing countries are designated with a Geographical BSE-Risk Level from GBR 

I to GBR IV. The Geographical BSE-Risk (GBR) is an indicator of the likelihood of the 

presence of one or more cattle being infected with BSE (Bovine Spongiform 

Encephalopathy), pre-clinically as well as clinically, at a given point in time. Where its 

presence is confirmed, the GBR gives an indication of the level of infection. 

Regulatory Compliance 

Countries vary in their guidelines for the import of FBS and its use in specific applications. 

Of primary concern is BSE and viruses. Countries in the GBR I and GBR II 

classification are those where BSE has not been detected and the risk is considered 

to be low; this is because animals are permitted to graze naturally. Countries in GBR 

Group III and IV are those where BSE has been detected or where the risk is 

considered to be high. 

Country of Origin 

This is defined as the country in which the donor/animal provides the serum. 

Biowest fetal bovine serum is sourced from the following countries : 


Presence of one or more cattle clinically or pre-clinically infected with the 

BSE agent in a geographical region/country 

I Highly unlikely 

II Unlikely but not excluded 

III Likely but not confirmed or confirmed, at a lower level 

IV Confirmed, at a higher level 

Cat N° S1500 Canada Cat N° S1600 Central America Cat N° S1750 Denmark 

Cat N° S1520 USA Cat N° S1650 Mexico Cat N° S1810 South America 

Cat N° S1560 Chile Cat N° S1700 Australia Cat N° S1820 France 

GBR I GBR II GBR III 

Argentina Brazil Canada 

Australia Colombia Chile 

New Zealand Costa Rica Denmark 

Panama Nicaragua France 

Paraguay Mexico 

Uruguay Spain 

USA

Animal Serum 

Presentation 

Filtration and Packaging 

Raw pooled FBS is filtered through a triple series of 0.1 micron sterilizing filters. The 

sterile serum is true-pooled to ensure homogeneity. 

Biowest products are packaged via an aseptic filling process for which each step has 

been determined to ensure the production of a product meeting the industry standard 

sterility assurance level of 104 (i.e., product that demonstrates a bacterial and 

fungal contamination level of no more than 1 of 10,000 units during the manufacturing 

process). The highest level of sterility assurance (106) cannot be achieved 

without terminal sterilization. Filtration and dispensing are performed within positive 

pressure, HEPA-filtered, environmentally controlled rooms. 

Final Filter Size : Triple 0.1 micron 

Sterility 

All sera are tested for the absence of aerobic and anaerobic bacteria, fungi, and yeast. 

Sterility test procedure is based on the European Pharmacopeia or US Pharmacopeia 

depending on the locale of final filtration. 

A representative number of samples from each production batch is selected for 

Sterility testing. 

Mycoplasma 

Each final product batch is tested for absence of mycoplasma. The sera are tested 

for the absence of Mycoplasma utilizing a 21 days cell culture assay in Axcell 

Biotechnologies media by culture method. Our test is accurate within limits of 

method detection. 

Virus Testing 

Each batch of bovine serum is tested for adventitious viruses using cell culture techniques. 

The serum is tested for: 

- Bovine Viral Diarrhoea (BVD) 

- Infectious Bovine Rhinotracheitis (lBR) 

- Parainfluenza Type 3 (Pl3) 

Sera are tested for the absence of the indicated viruses by inoculation with GBK cells. 

The revelation is made by indirect immunofluorescence. 

Antibody Testing : presence of specific antibodies is detected utilizing an Elisa Assay. 

The serum from equidea is tested for the presence of Equine Infectuous Anemia 

antibodies. 


07

Animal Serum 

08 

Animal Serum 

Presentation 

Endotoxin Test (LAL) 

All sera are tested to determine and quantify endotoxin levels. 

BioWest conducts two tests : 

1. Turbidimetric - a qualitative assay for FBS. 

2. Chromokinetic - a quantitative test for all products. 

The endotoxin reagent is standardized against the US reference endotoxin. 

Test method is based on " the bacterial endotoxins test" , US Pharmacopeia 23rd 

revision. 

Haemoglobin 

To verify that proper collection and processing procedures have been followed, a 

quantitative and colorimetric assay is performed to determine the residual 

haemoglobin concentration in each product batch. 

Total Protein 

Determined by Electrophoresis. 

Osmolarity 

Osmolarity is determined by the lowered freezing temperature. The osmometer is 

calibrated using traceable standards. 


Protein Electrophoresis Methodology 

Protein Biuret colorimetry 

Albumin Immunoturbidimetry 

Globulin Immunoturbidimetry

Animal Serum 

Presentation 

Cell Culture Testing 

Each batch of serum is tested for its ability to support in vitro growth of specific 

cell lines. Therefore, in addition to verify that each batch of sera passes our exacting 

quality control specifications, three important performance criteria are evaluated in 

our Quality Control Program: 

Growth Promotion 

Cloning Efficiency 

Plating Efficiency 

Biological performance is assessed using cell culture medium supplemented with a 

final concentration of 10% serum. During the test period, cultures are examined 

microscopically for any morphological abnormalities that may indicate toxic components 

in the serum. 

The following cell lines are utilized to determine growth promotion and functionality: 

Cell Line Type Species 

HeLa Cancer Human 

L929 Fibroblast, Macrophage Mouse 

Sp2/0-Ag14 Lymphoma Mouse 

MRC-5 Lung Human 

pH 

All pH meters are daily calibrated with standard solutions. 

Bovine Spongiform Encephalopathy (BSE) Testing 

According to the European Regulation EC n° 999/2001, female donor animals are 

tested for BSE before the corresponding fetal blood is allowed to be processed. 

EU is pioneer in BSE testing and individual identification of animals through ear 

tagging, which ensures the best possible traceability and the lowest BSE risk and makes 

the EU origin the first choice of researchers in Japan and other selective markets. 

TEST Prionics Bio-Rad 

METHOD Western Blot ELISA 


09

Animal Serum 

10 

Animal Serum 

Presentation 

Chemical and Enzyme Profile 

Storage and Shelf Live 

Animal Sera and Plasma is stored at -20°C. The shelf life for animal Serum is 

60 months, and for animal plasma 48 months. 


Items Methods 

Sodium Indirect potentiometry 

Potassium Indirect potentiometry 

Chloride Indirect potentiometry 

Calcium Arsénazo colorimetry 

Phosphorus Phosphomolybdate colorimetry 

Alkaline Phosphatase Colorimetry kinetic at 37°C 

LDH UV kinetic at 37°C 

SGOT UV kinetic at 37°C 

SGPT UV kinetic at 37°C 

Gamma GT Colorimetry kinetic at 37°C 

Cholesterol Cholesterase Trinder colorimetry 

Bilirubin DPD / cafeine colorimetry 

Glucose Hexokinase UV 

Urea Urease UV 

Creatinine Colorimetry Kinetic (Jaffé) 

Triglyceride Glycerokinase Trinder colorimetry 

Iron TPTZ colorimetry

Animal Serum 

Certificate of Analysis 


11

Animal Serum 

12 

Animal Serum 

Treatments 

Heat Inactivation (56°C - 30 min) : 

This treatment allows to destroy the complement acting in the immune 

system to avoid an interference with some experimentation, in particular 

the tests of immunology. It allows also to inactivate viruses and to destroy 

some bacterial contaminants such as the mycoplasma. 

For orders please add 


For orders please add code H 

Ex : FBS origin South America : S1810 

Heat inactivated : S181H 

Gamma Irradiation : 

The serum is gamma irradiated at 25 kGy. 

Gamma irradiated serum minimizes the risk associated with the use of 

animal products and offers protection against low levels of microbial 

contaminants. 

The treatment inactivates the viruses of foot and mouth disease, vesicular 

stomatitis, rinderpest, peste des petits ruminants, Rift valley fever, 

bluetongue… while maintaining growth promotion potential. 

For orders please add code G 

Ex : FBS origin Canada : S1500 

Gamma Irradiated : S150G 

Charcoal Stripping : 

BioWest offers Charcoal / Dextran stripped Fetal Bovine serum for 

researchers requiring low levels of hormones. 

Charcoal / Dextran stripping reduces the concentration of steroid 

hormones in serum such as estradiol, progesterone, cortisol, testosterone, 

T3 and T4. 

This serum is usefull where there endogenous molecules may interfere 

with experimental works. This serum may show some reduced growth 

promotion ability for cells requiring the presence of certain hormones 

(Biblio: Northbio). 

For orders please add code F 

Ex : FBS origin France : S1820 

Charcoal stripped : S182F 

Dialysation : 

The dialysis reduces the concentration of free low molecular weight components 

such as nucleotides, amino acids, hormones and ions. We use a 

dynamic filtration method to produce our dialysed serum. The sera are 

dialysed using a 10 000 molecular weight cut-off membrane. 

For orders please add code D 

Ex : FBS origin Australia : S1700 

Dialysed : S170D

Animal Serum 

Treatments 

IgG low ( < 5 mg / l) : 

The IgG level in the fetal bovine serum is in general between 50 and 

200mg/l, it is a low level compared to the other sera as the New Born 

Calf serum. However, this level can cause some problems for some utilisations, 

that is why we select the sera with a level < 5mg/l for the users. 

For orders please add code I 


IgG low : S181I 

Lipid Depletion : 

Biowest uses the fumed silica precipitation method for removing lipids. 

The fumed silica powder is added to the serum. It’s well mixed together. 

After the solution is centrifuged. The supernatant contains the serum and 

the lipids are with the silica in the pellet. 

For orders please add code L 

Ex : FBS origin Mexico : S1650 

Lipid depleted : S165L 

Calcium Stripping : 

The calcium depletion is achieved using chromatography columns (ionexchange 

resin). This resin is selective for divalent cations over monovalent 

cations (It follows that copper, iron, magnesium, zinc etc. levels will 

be lowered too) . 

This serum may show some reduced growth promotion of cells requiring 

the presence of calcium or other divalent cations. 

For orders please add code C 

Ex : FBS origin Denmark : S1750 

Calcium stripped : S175C 

Tetracycline : 

The serum is tested for the presence of Chlortetracycline, Oxytetracycline 

and Tetracycline by a liquid chromatography electrospray ionisation 

tandem mass spectrometry method. The detection limit is < 0.05 mg/l. 

For orders please add code T 

Ex : FBS origin France : S1820 

Tetracycline free : S182T 

Iron Supplementation : 

A 1% Ferric citrate sterile solution is added to the serum at 0,246% 

(v/v). Then the serum is well mixed. Transferrin is normally 20 to 40 % 

bound to iron. 

For orders please add code R 


Iron supplemented : S181R 


13

Fetal Bovine Serum 

14 

Fetal Bovine Serum 

Product list 


Cat N° Description Unit / Size 

S1810 Fetal Bovine Serum 100 ml 

South America Origin 500 ml 

State : Frozen Storage : -20° C Life : 60 months 

S1820 Fetal Bovine Serum BSE tested 100 ml 

France Origin 500 ml 



Denmark Origin 500 ml 



Canada Origin 500 ml 



USA Origin 500 ml 



Mexico, USDA Origin 500 ml 



Central America Origin 500 ml 



Chile Origin 500 ml 



Australia Origin 500 ml 



South America Origin, EDQM certified 500 ml 


>> Treatments see page 12 -13


S0250 Bovine Serum (France Origin) 100 ml 

State : Frozen Storage : -20° C Life : 60 months 500 ml 

S1400 Bull Calf Serum 100 ml 


S0400 Calf Serum 100 ml 


S0600 Donor Bovine Serum 100 ml 


S0450 Donor Calf Serum 100 ml 


S0750 New Born Calf Serum 100 ml 


S0800 Donor Foal Serum 100 ml 


S0900 Donor Horse Serum 100 ml 


S0950 Donor Horse Serum (from Pregnant Horses) 100 ml 


S0960 Fetal Horse Serum 100 ml 


S0910 Horse Serum 100 ml 


S2170 Donkey Serum 100 ml 


S2000 Goat Serum 100 ml 


S2300 Lamb Serum 100 ml 


S2350 Sheep Serum 100 ml 


>> Treatments see page 12 -13 

Animal Serum 

Product list 


15

Animal Serum 

16 

Animal Serum 

Product list 

Animal Plasma 

Product list 


S2500 Rabbit Serum (France Origin) 100 ml 


S0500 Chicken Serum 100 ml 


S2400 Pig Serum 100 ml 


S2450 Guinea Pig Serum 20 ml 


S2150 Rat Serum 20 ml 


S2160 Mouse Serum 20 ml 


S2800 Cat (Feline) Serum 2 ml 


S2900 Dog (Canine) Serum 10 ml 


S2901 Dog (Canine) Beagle Serum 10 ml 



S0260 Bovine Plasma w/ Sodium Citrate 100 ml 


S0410 Calf Plasma w/ Sodium Citrate 100 ml 


S0920 Horse Plasma w/ Sodium Citrate 100 ml 


S2010 Goat Plasma w/ Sodium Citrate 100 ml 


S2310 Lamb Plasma w/ Sodium Citrate 100 ml 


>> Treatments see page 12 -13

S2360 Sheep Plasma w/ Sodium Citrate 100 ml 


S2600 Rabbit Plasma w/ EDTA 100 ml 


S2410 Pig Plasma w/ Sodium Citrate 100 ml 


S2140 Rat Plasma w/ Lithium Heparin 100 ml 


S2162 Mouse Plasma w/ Lithium Heparin 100 ml 


S2910 Dog (Canine) Plasma w/ Sodium Citrate 100 ml 


Animal Plasma 

Product list 


17

Albumin Bovine 

18 

Albumin Bovine 


Presentation 

Product list 

Bovine serum albumin (BSA) is commonly used in cell culture protocols, 

particularly where protein supplementation is necessary and the other 

components of serum are unwanted. In cell culture, its main role is as a 

carrier of small molecules. Because of its negative charge, BSA binds 

water, salts, fatty acids, vitamins and hormones, then carries these bound 

components between tissues and cells. The binding capacity also makes 

BSA an effective scavenger to remove toxic substances, including 

pyrogens, from the medium. 

Albumins are readily soluble in water and can only be precipitated by 

high concentrations of neutral salts such as ammonium sulfate. The 

solution stability of BSA is very performant (especially if the solutions 

are stored as frozen aliquots). Albumins are frequently used as stabilizers 

for other solubilized proteins (e.g., labile enzymes). However, albumin is 

readily coagulated by heat. When heated to 50°C or above, albumin 

quite rapidly forms hydrophobic aggregates which do not revert to 

monomers upon cooling. At somewhat lower temperatures aggregation 

is also expected to occur, but at relatively slower rates. 

Albumin is used to solubilize lipids, and is also used as a blocking agent 

in Western Blot or ELISA applications. 


Liquid 

P6150 Albumin Bovine Serum Standard Quality Lyophilised 10 g 

State : powder Storage : +2/+8 °C Life : 48 months 100 g 

500 g 

P6152 Albumin Bovine Serum Fraction V 10 g 

State : powder Storage : +2/+8 °C Life : 48 months 100 g 

500 g 

>> BSA in solution and Charcoal stripped also available on request

Cell Culture Medium 

Presentation 

BioWest media formulations are manufactured following original 

publications, standards set by the Tissue Culture Association and 

accepted formulations. These formulations may vary from these 

standards by substituting hydrated, chlorinated and/or the salt forms of 

certain compounds where such substitutions contribute to improved 

performance of the product. 

Custom Made Formulations 

The realisation and production of your personalised cell culture media 

compositions is another of our services. In your catalogue you will find 

a list of substances for cell culture media. Give us the list of components 

needed and the corresponding quantities and we will produce this to 

your requirements. There may be a short delay for this service. 

Quality Assurance 

All of the chemicals, raw materials and equipments that we use is of the 

highest quality. Inorganic chemicals are analytical, ACS, USP, EP, FCC 

grade or otherwise the finest grade available. All other components are 

evaluated by standards established by BioWest. 

All new batches of chemicals are introduced in the process after 

Quality Control. The water used for media preparation is of the best 

quality. It is of WFI quality, produced in several steps including 

centrifugal distillation and tested for endotoxin. The resistivity is 

measured in line and all values are monitored and stored by the 

Quality Control department. The water is always freshly processed and 

cooled down to 20°C before adding the powders. 

Equipment and Conditions 

All equipments used for the manufacturing of powdered and liquid 

media are made of chemical inert materials which will not contaminate 

the final product. For 20 years BioWest has produced for cell culture following 

strict environmental conditions regarding cleanliness and moisture. 

Humidity and temperature are monitored constantly to guaranty that 

all chemicals are ground into fine powder. The liquid media sterilisation 

is carried out by going through a 0,1 µm pore size sterile filter. 

Batch Size 

Batch sizes for powdered media range from 1 to 10.000 liters, for liquid 

media from 1 to 1500 liters. 


19

20 

Presentation 

Cell Culture Medium Cell Culture Medium 

End Product Testing 

The powder Media are tested physically for osmotic effects and pH. 

Osmolality is determined by means of the freezing point method. 

The chemical composition and the homogeneity of the mixture are 

controlled by analysis of glucose or sodium in the sample. Endotoxin 

testing is performed using the Limulus Amoebocyte test. The chromokinetic 

test is used to verify the quality of the medium. 

The liquid Media are tested for the sterility, pH and osmolality. 

The biological performance test is done on different cell lines (following 

the medium). The endotoxin level is also tested by a chromokinetic test. 

Storage and Stability 

Powdered media must be stored at + 2 / +8°C in their original containers 

and in dry, dark conditions. Heat, light and humidity can greatly 

affect the performance of powdered media therefore we recommend that 

powder be stored correctly if not used entirely. All liquid media must be 

stored at + 2 / +8°C in the dark. 

Advantages and use of powdered tissue culture 

media 

While liquid media are convenient to use, there are several drawbacks 

which makes powdered media also attractive : 


1. Long term studies can be carried out using a single batch of 

powdered medium 

2. Storage time higher 

3. Reduces the unit costs by 3-10 times 

4. Storage space is greatly reduced 

The advantages of liquid media : 

1. Lower labour costs 

2. Quality control and functional testing 

3. Stock organisation is easier to control 

The protocols of all steps during processing and all final test results 

reassure the customer that each batch meets the criteria and has been 

manufactured to the product specifications. The retained samples allow 

also the testing of long term shelf-life studies and also control in response 

to customer inquiries.


Summary 

Cell Culture Medium . . . . . . . . . . . . . . . . . .page # 

Basal Medium Eagle (BME) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 

CMRL 1066 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 

Dulbecco’s Modified Eagle’s Medium (DMEM) . . . . . . . . . . 23 - 25 

DMEM High Glucose . . . . . . . . . . . . . . . . . . . . . . . . 23 - 24 

DMEM Low Glucose . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 

DMEM – Ham’s F12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 

Glasgow MEM BHK 21 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 - 26 

Ham’s F10 ( F-10 Nutrient Medium) . . . . . . . . . . . . . . . . . . . . . . 26 



Iscove’s Modified Dulbecco’s Medium (IMDM) . . . . . . . . . . . . . . 28 

Leibovitz L15 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 

Mc COY’s 5A Medium modified . . . . . . . . . . . . . . . . . . . . . . . . . . 29 

Minimum Essential Medium (MEM) . . . . . . . . . . . . . . . . . . . 30 - 32 

MEM w/ Earle’s Salts . . . . . . . . . . . . . . . . . . . . . . . . 30 - 31 

MEM w/ Hanks’ Salts . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 

MEM Alpha Modification . . . . . . . . . . . . . . . . . . . . . 31 - 32 

Medium 199 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 

RPMI 1640 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 - 34 

Schneider’s Drosophila Medium . . . . . . . . . . . . . . . . . . . . . . . . . 35 

Serum Reduced Medium - MCDB . . . . . . . . . . . . . . . . . . . . . . . . 35 

Compositions . . . . . . . . . . . . . . . . . . . . . . . . . . 36 - 54 

Preparation Instruction . . . . . . . . . . . . . . . . . . . . . . 55 

Custom Made Formulation . . . . . . . . . . . . . . . . 56 - 59 

Table of Addition of L-Glutamine . . . . . . . . . . 60 - 61 

Table of Addition of Sodium Bicarbonate . . . . 62 - 64 


21

22 

Product List 

Basal Medium Eagle (BME) 


Liquid 

L0042 BME w/ Earle's Salts w/o L-Glutamine 100 ml 

State : Liquid Storage : +2/+8 °C Life : 24 months 500 ml 

L0044 BME w/ Earle's Salts w/o L-Glutamine w/ 25 mM Hepes 100 ml 


L0043 BME w/ Earle's Salts w/ L-Glutamine 100 ml 


L0045 BME w/ Earle's Salts w/ L-Glutamine w/ 25 mM Hepes 100 ml 


L0046 BME w/ Hanks' Salts w/o L-Glutamine 100 ml 


Powder 

P0030 BME w/ Earle's Salts w/ L-Glutamine For 1 L 

w/o Sodium Bicarbonate For 5 L 

State : Powder Storage : +2/+8 °C Life: 48 months For 10 L 

P0031 BME w/ Hanks' Salts w/ L-Glutamine For 1 L 


State : Powder Storage : +2/+8 °C Life: 48 months For 10 L 



10x concentration 

>> See composition page 36 

Basal Medium Eagle is principaly used for diploid or primary cell cultures. It is the 

simplest of the basal media with all essential components for growth. BME ideally 

supports cell lines such as HeLa, L-cells and primary mammalian fibroblasts. 

X0042 BME 10X w/ Earle's Salts w/o L-Glutamine 100 ml 

w/o Sodium Bicarbonate 500 ml 

State : Liquid Storage : +2/+8 °C Life : 24 months 

X0026 BME 10X w/ Hanks' Salts w/o L-Glutamine 100 ml 



See page 59-63 for addition of L-Glutamine 

and Sodium Bicarbonate

CMRL 1066 


and Sodium Bicarbonate 


Product list 


Liquid 

L0053 CMRL 1066 w/o L-Glutamine 100 ml 


Powder 

P0058 CMRL 1066 w/ L-Glutamine w/o Sodium Bicarbonate For 1 L 

State : Powder Storage : +2/+8 °C Life : 36 months For 5 L 

For 10 L 

DMEM High Glucose 


Liquid 


CMRL was originally formulated for promoting " L" Earle cells growth. 

Dulbecco's Modified Eagle Medium (DMEM) 

Dulbecco's Modified Eagle Medium (DMEM) is for supporting and maintening a broad 

spectrum of mamalian cell types. 


L0101 DMEM High Glucose w/o L-Glutamine 100 ml 

w/o Sodium Pyruvate 500 ml 


L0106 DMEM High Glucose w/o L-Glutamine w/ Sodium Pyruvate 100 ml 


L0100 DMEM High Glucose w/o L-Glutamine w/ 25mM Hepes 100 ml 


L0102 DMEM High Glucose w/ L-Glutamine w/o Sodium Pyruvate 100 ml 


L0104 DMEM High Glucose w/ L-Glutamine w/ Sodium Pyruvate 100 ml 


L0103 DMEM High Glucose w/ stable Glutamine 100 ml 

w/ Sodium Pyruvate 500 ml 


L0107 DMEM High Glucose w/ stable Glutamine w/ 25mM Hepes 100 ml 



23

24 

Powder 

Product list 

P0102 DMEM High Glucose w/ L-Glutamine For 1 L 

w/o Sodium Bicarbonate w/ Sodium Pyruvate For 5 L 


P0103 DMEM High Glucose w/ L-Glutamine For 1 L 

w/o Sodium Bicarbonate w/o Sodium Pyruvate For 5 L 


DMEM Low Glucose 


Liquid 

L0064 DMEM Low Glucose w/o L-Glutamine w/ Sodium Pyruvate 100 ml 


L0067 DMEM Low Glucose w/o L-Glutamine 100 ml 

w/ Sodium Pyruvate w/ 25 mM Hepes 500 ml 


L0060 DMEM Low Glucose w/ L-Glutamine w/ Sodium Pyruvate 100 ml 


L0065 DMEM Low Glucose w/ L-Glutamine 100 ml 

w/ Sodium Pyruvate w/ 25mM Hepes 500 ml 


L0070 DMEM Low Glucose w/ L-Glutamine 100 ml 

w/o Sodium Bicarbonate w/ Sodium Pyruvate 500 ml 


L0066 DMEM Low Glucose w/ stable Glutamine 100 ml 

w/ Sodium Pyruvate 500 ml 


Powder 




P0061 DMEM Low Glucose w/ L-Glutamine For 1 L 

w/o Sodium Bicarbonate w/ Sodium Pyruvate For 5 L 




DMEM - Ham's F12 >> See composition page 40 




Product List 

It offers excellent performance for certain epithelial, endothelial and granulosa cell types. With 

proper supplementation, it is a highly successful basic media for serum - free cell culture. 


Liquid 

L0090 DMEM F12 w/o L-Glutamine w/o Hepes 100 ml 


L0091 DMEM F12 w/o L-Glutamine w/o Hepes w/o Glucose 100 ml 


L0094 DMEM F12 w/o L-Glutamine w/ 15 mM Hepes 100 ml 


L0096 DMEM F12 w/o L-Glutamine w/ 25 mM Hepes 100 ml 


L0093 DMEM F12 w/ L-Glutamine w/ 15 mM Hepes 100 ml 


L0095 DMEM F12 w/ L-Glutamine w/ 25 mM Hepes 100 ml 


L0092 DMEM F12 w/ stable Glutamine w/ 15 mM Hepes 100 ml 


Powder 

P0095 DMEM F12 w/ L-Glutamine w/o Sodium Bicarbonate For 1 L 

w/ 15 mM Hepes For 5 L 


Glasgow MEM BHK 21 

GMEM was originally formulated for BHK 21 cells growing. It is intended for use with 

adherent cell lines. 


Liquid 


L0222 Glasgow MEM BHK 21 w/o L-Glutamine 100 ml 

w/o Tryptose Phosphate Broth 500 ml 


L0221 Glasgow MEM BHK 21 w/ L-Glutamine 100 ml 




25

26 

Product List 

Powder 

P0120 Glasgow MEM BHK 21 w/ L-Glutamine w/o Sodium For 1 L 

Bicarbonate w/o Tryptose Phosphate Broth For 5 L 



X0123 Glasgow MEM BHK 21 10X w/o L-Glutamine 100 ml 



X0122 Glasgow MEM BHK 21 10X w/ L-Glutamine 100 ml 



Ham'S F10 (F-10 Nutrient Medium) 


Liquid 

L0145 Ham's F10 w/o L-Glutamine 100 ml 


L0140 Ham's F10 w/ L-Glutamine 100 ml 


L0130 Ham's F10 w/ L-Glutamine w/ 25 mM Hepes 100 ml 


Powder 

P0146 Ham's F10 w/ L-Glutamine w/o Sodium Bicarbonate For 1 L 


For 10 L 



X0150 Ham's F10 10X w/ L-Glutamine w/o Sodium Bicarbonate 100 ml 

State : Powder Storage : +2/+8 °C Life : 12 months 500 ml 



To support the growth of Chinese Hamster Ovary Cells under serum free conditions and other 

mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious 

cell lines. Ham’s F-10 has been shown to support the growth of human diploid cells, white 

blood cells for chromosomal analysis, primary explants of rat, rabbit and chicken tissues. 






Product List 

Ham'S F12 (F-12 Nutrient Medium) >> See composition page 43 

Ham's F12 Medium was originally developed for single-cell plating of near diploid CHO 

cells. It is a rich modification of F-10 medium designed for the cloning and serial 

propagation of CHD-3 and CHL-1 cell lines in the absence of serum. It also offers 

enhanced performance for cells growing at low density. F-12 features increased levels of 

choline, I-inositol, putrescine and several amino acids. It is excellent for cultivating 

carcinoma cells, rat skeletal myoblasts, lungs cells and rat, rabbit or chicken embryos. 


Liquid 

L0136 Ham's F12 w/o L-Glutamine 100 ml 

State : LiquidStorage : +2/+8 °C Life : 24 months 500 ml 

L0133 Ham's F12 w/o L-Glutamine w/ 25 mM Hepes 100 ml 


L0135 Ham's F12 w/ L-Glutamine 100 ml 


L0132 Ham's F12 w/ L-Glutamine w/ 25 mM Hepes 100 ml 


Powder 

P0134 Ham's F12 w/ L-Glutamine w/o Sodium Bicarbonate For 1 L 


For 10 L 


X0155 Ham's F12 10X w/o L-Glutamine w/o Sodium Bicarbonate 100 ml 



27

28 

Product List 

Ham'S F14 (F-14 Nutrient Medium) 

Ham’s Nutrient Mixtures were originally developed to support clonal growth of several 

clones of Chinese hamster ovary (CHO) cells, as well as clones of HeLa and mouse L-cells. 

Both mixtures were formulated for use with or without serum supplementation, depending 

on the cell type being cultured. Ham’s F14 has been used to support the culture of neonatal 

dorsal root ganglion. 


Liquid 

L0138 Ham's F14 w/ 6 g/L Glucose w/ 1mg/L ATP 100 ml 


Iscove's Modified Dulbeccos' Medium (IMDM) 


Liquid 

L0192 IMDM w/o L- Glutamine w/o Hepes 100 ml 


L0190 IMDM w/ L- Glutamine w/ 25 mM Hepes 100 ml 


L0191 IMDM w/ stable Glutamine w/ 25 mM Hepes 100 ml 


Powder 


P0191 IMDM w/ L- Glutamine w/o Sodium Bicarbonate For 1 L 

w/ 25 mM Hepes For 5 L 




Iscove's Media are enriched modifications of DMEM containing sodium selenite. They are 

excellent for rapidly proliferating, high density cell cultures. The addition of BSA, purified 

human Transferrin and soybean lecithin creates serum - free conditions ideal for supporting 

B and T lymphocytes. IMDM was the first media utilizing Hepes Buffer. Other cell types 

can be cultured using this medium under serum-free or reduced conditions. 



>> See composition page 45

Leibovitz L-15 Medium 




Product List 

The Leibovitz L-15 media were formulated to promote the cell growth in medium not 

balanced in CO2. The formulations were developed with the sodium bicarbonate buffer. 

The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and 

galactose so they can be used under conditions of free gaseous exchange with the 

atmosphere. When properly supplemented, L-15 Medium supports established cell lines, 

such as HEp-2 and LLC-MK 2, as well as primary explants of embryonic and adult human 

tissue. Many viruses also have been successfully cultivated in this medium. 


Liquid 

L0300 Leibovitz L-15 Medium w/o L-Glutamine 100 ml 


Powder 

P0350 Leibovitz L-15 Medium w/ L-Glutamine For 1 L 


For 10 L 

Mc Coy's 5A 


Liquid 

L0211 Mc Coy's 5A w/o L-Glutamine 100 ml 


L0210 Mc Coy's 5A w/ L-Glutamine 100 ml 


Powder 



Mc Coy's media were originally formulated for growth and support of lymphocytes. This 

final modification produced a medium identical to RPMI 1629. Mc Coy's 5A media support 

the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the 

propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or 

transformed cell types, the most current primary and continuous cell lines. 

P0390 Mc Coy's 5A w/ L-Glutamine w/o Sodium Bicarbonate For 1 L 


For 10 L 


29

30 

Product List 

Minimum Essential Media (MEM) 

Modification of BME featuring increased amino acids levels to more closely resemble the 

protein content of human cells. MEM serves as a general used medium ideal for the 

growth and maintenance of a wide range of mammalian cell types. Often used to support 

anchorage-dependant cells, modified formulations can be used to support other cell types 

including calcium-free MEM for suspension cultures and MEM with Hank's Salts for 

dipoid cells. 

MEM w/ Earle's Salts >> See composition page 47 


Liquid 

L0440 MEM w/ Earle's Salts w/o L-Glutamine 100 ml 


L0445 MEM w/ Earle's Salts w/o L-Glutamine w/ 25mM Hepes 100 ml 


L0430 MEM w/ Earle's Salts w/o L-Glutamine w/ NEAA 100 ml 


L0415 MEM w/ Earle's Salts w/ L-Glutamine 100 ml 


L0444 MEM w/ Earle's Salts w/ L-Glutamine w/ 25mM Hepes 100 ml 


L0416 MEM w/ Earle's Salts w/ stable Glutamine 100 ml 


Powder 

P0450 MEM w/ Earle's Salts w/ L-Glutamine w/ NEAA For 1 L 



P0451 MEM w/ Earle's Salts w/ L-Glutamine For 1 L 

w/o NEAA w/o Sodium Bicarbonate For 5 L 



P0452 MEM w/ Earle's Salts w/ L-Glutamine For 1 L 

w/o Sodium Bicarbonate w/ 25mM Hepes For 5 L 








Product List 

P0460 MEM Autoclavable w/ Earle's Salts w/o L-Glutamine For 1 L 




X0311 MEM 10X w/ Earle's Salts w/o L-Glutamine 100 ml 



MEM w/ Hanks' Salts 


Liquid 

L0465 MEM w/ Hanks' Salts w/o L-Glutamine 100 ml 


L0470 MEM w/ Hanks' Salts w/o L-Glutamine w/ 25mM Hepes 100 ml 


Powder 

P0515 MEM w/ Hanks' Salts w/ L-Glutamine w/ NEAA For 1 L 




X0321 MEM 10X w/ Hank's Salts w/o L-Glutamine 100 ml 



MEM Alpha Modification 


Liquid 



L0476 MEM Alpha w/o L-Glutamine w/o Ribunocleosides 100 ml 

w/o Deoxyribonucleosides 500 ml 


L0475 MEM Alpha w/ L-Glutamine w/o Ribunocleosides 100 ml 

w/o Deoxyribonucleosides 500 ml 



31

32 

Medium 199 

Product List 

Powder 

P0440 MEM Alpha Modification w/ Earle's Salts w/ L-Glutamine For 1 L 




Liquid 

L0325 Medium 199 w/ Hanks' Salts w/o L-Glutamine 100 ml 


L0330 Medium 199 w/ Hanks' Salts w/ L-Glutamine 100 ml 

State : Liquid Storage : +2/+8 °C Life: 18 months 500 ml 

L0331 Medium 199 w/ Hanks' Salts w/ L-Glutamine 100 ml 

w/ 25mM Hepes 500 ml 

State : Liquid Storage : +2/+8 °C Life: 18 months 

L0356 Medium 199 w/ Earle’s Salts w/o L-Glutamine 100 ml 


L0355 Medium 199 w/ Earle's Mod. Salts w/ L-Glutamine 100 ml 

w/ 1,25 g/L Sodium Bicarbonate 500 ml 


L0360 Medium 199 w/ Earle's Salts w/ L-Glutamine 100 ml 

w/ 25mM Hepes 500 ml 


Powder 

P0420 Medium 199 w/ Earle's Salts w/ L-Glutamine For 1 L 




P0425 Medium 199 w/ Earle’s Salts w/ L-Glutamine For 1 L 

w/o Sodium Bicarbonate w/ 25mM Hepes For 5 L 


P0410 Medium 199 w/ Hanks' Salts w/o L-Glutamine For 1 L 





This complex medium was originally developed specifically for nutritional research of chicken 

fibroblasts. Today, 199 media are widely used for the maintenance of non-transformed 

cells, vaccine and virus production, and primary explants of epithelial cells. 



RPMI 1640 Medium 




Product List 


Liquid 

>> See composition page 52 - 53 

RPMI are general purpose enriched media with extensive applications for a broad spectrum 

of mammalian and hybridoma cells including human myeloma, mouse hybridoma, human 

leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures 

and monolayer culture of human leukemic cells. 

L0501 RPMI 1640 w/o L-Glutamine 100 ml 


L0490 RPMI 1640 w/o L-Glutamine w/ 25 mM Hepes 100 ml 


L0505 RPMI 1640 w/o L-Glutamine w/o Phenol Red 100 ml 


L0503 RPMI 1640 w/o L-Glutamine w/o Folic Acid 100 ml 


L0500 RPMI 1640 w/ L-Glutamine 100 ml 


L0495 RPMI 1640 w/ L-Glutamine w/ 25mM Hepes 100 ml 


L0504 RPMI 1640 w/ L-Glutamine w/o Sodium Bicarbonate 100 ml 


L0492 RPMI 1640 Dutch Modification w/o L-Glutamine 100 ml 

w/ 1g/l Sodium Bicarbonate w/ 20mM Hepes 500 ml 


L0498 RPMI 1640 w/ stable Glutamine 100 ml 


L0496 RPMI 1640 w/ stable Glutamine w/ 25mM Hepes 100 ml 



33

34 

Product List 

POWDER 

P0870 RPMI 1640 w/o L-Glutamine w/o Sodium Bicarbonate For 1 L 


For 10 L 


w/o Phenol Red For 5 L 



w/ 25mM Hepes For 5 L 


P0860 RPMI 1640 w/ L-Glutamine w/o Sodium Bicarbonate For 1 L 


For 10 L 





w/o Glucose For 5 L 



w/25 mM Hepes w/o Phenol Red For 5 L 


P0877 RPMI 1640 w/ stable Glutamine w/25 mM Hepes For 1 L 


State : Powder State : +2/+8 °C Life : 24 months For 10 L 





Schneider's Drosophila Medium 




Product List 

Schneider’s Insect medium was developed to support the growth of excised imaginal disks 

from the fruitfly, Drosophila melanogaster. When supplemented with 5-20% heat-inactivated 

fetal bovine serum, Schneider’s medium has been found to support the rapid growth 

of both primary and established cultures of cells derived from Drosophila spp. and several 

other dipterans. 


Liquid 

L0207 Schneider's Drosophila Medium 100 ml 


Serum Reduced Media - MCDB 


Liquid 


These media were formulated as a reduced serum supplemented medium for the cultivation 

of human microvascular endothelials cells. For other microvascular cells it is recommended 

to use it with growth factors like EGF or hormons like hydorcortisone. 

L1200 MCDB 105 100 ml 


L1202 MCDB 131 100 ml 


L1203 MCDB 151 100 ml 


L1205 MCDB 201 100 ml 


L1206 MCDB 302 100 ml 



35



O.C.* Vitamins 

Inorganic Salts Amino Acids 

36 

Product Composition 

Basal Medium Eagle (BME) L0042 L0043 L0044 L0045 L0046 P0030 P0031 X0026 X0042 

Liquid Liquid Liquid Liquid Liquid Powder Powder Liquid Liquid 

mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l 

L-Arginine Monohydrochloride 21 21 21 21 21 21 21 210 210 

L-Cystine Dihydrochloride 15,65 15,65 15,65 15,65 15,65 15,65 15,65 156,5 156,5 

L-Glutamine / 292 / 292 / 292 292 / / 

L-Histidine 8 8 8 8 8 8 8 80 80 

L-Isoleucine 26 26 26 26 26 26 26 260 260 

L-Leucine 26 26 26 26 26 26 26 260 260 

L-Lysine Monohydrochloride 36,47 36,47 36,47 36,47 36,47 36,47 36,47 364,7 364,7 

L-Methionine 7,5 7,5 7,5 7,5 7,5 7,5 7,5 75 75 

L-Phenylalanine 16,5 16,5 16,5 16,5 16,5 16,5 16,5 165 165 

L-Threonine 24 24 24 24 24 24 24 240 240 

L-Tryptophan 4 4 4 4 4 4 4 40 40 

L-Tyrosine Disodium Salt Dihydrate 25,95 25,95 25,95 25,95 25,95 25,95 25,95 259,5 259,5 

L-Valine 23,5 23,5 23,5 23,5 23,5 23,5 23,5 235 235 

Calcium Chloride Dihydrate 265 264 265 265 185 264 185 1850 2650 

Magnesium Sulfate Anhydrous 97,67 97,67 97,67 97,67 97,67 97,67 97,67 976,7 976,7 

Potassium Chloride 400 400 400 400 400 400 400 4000 4000 

Potassium Phosphate Monobasic Anhydre / / / / 60 / 60 600 / 

Sodium Bicarbonate 2200 2200 2200 2200 350 / / / / 

Sodium Chloride 6800 6800 6800 6800 8000 6800 8000 80000 68000 

Sodium Phosphate Dibasic Anhydrous / / / / 47,88 / 47,88 478,8 / 

Sodium Phosphate Monobasic Anhydrous 122 122 122 122 / 122 / / 1220 

Choline Chloride 1 1 1 1 1 1 1 10 10 

D-Biotin 1 1 1 1 1 1 1 10 10 

D-Ca Pantothenate 1 1 1 1 1 1 1 10 10 

Folic Acid 1 1 1 1 1 1 1 10 10 

Myo-Inositol 2 2 2 2 2 2 2 20 20 

Nicotinamide (Nicotinic acid amide) 1 1 1 1 1 1 1 10 10 

Pyridoxal Hydrochloride 1 1 1 1 1 1 1 10 10 

Riboflavin 0,1 0,1 0,1 0,1 0,1 0,1 0,1 1 1 

Thiamine Hydrochloride 1 1 1 1 1 1 1 10 10 

D-Glucose 1000 1000 1000 1000 1000 1000 1000 10000 10000 

Hepes Free Acid / / 5958 5958 / / / / / 

Phenol Red Sodium Salt 11 11 11 11 11 11 11 110 110 


* Other Components

Amino Acids 

Inorganic Salts 

Vitamins 

Other Components 

CMRL 1066 L0053 P0058 

Liquid Powder 

mg/l mg/l 

Glycine 50 50 

L-Alanine 25 25 

L-Arginine Free Base / 57,87 

L-Arginine Monohydrochloride 70 / 

L-Aspartic acid 30 30 

L-Cysteine Monohydrochloride Monohydrate 260 260 

L-Cystine 20 / 

L-Cystine Dihydrochloride / 20 

L-Glutamic Acid 75 75 

L-Glutamine / 100 

L-Histidine Monohydrochloride Monohydrate 20 20 

L-Hydroxy-L-Proline 10 10 

L-Isoleucine 20 20 

L-Leucine 60 60 

L-Lysine Monohydrochloride 70 70 

L-Methionine 15 15 

L-Phenylalanine 25 25 

L-Proline 40 40 

L-Serine 25 25 

L-Threonine 30 30 

L-Tryptophan 10 10 

L-Tyrosine 40 40 

L-Valine 25 25 

Calcium Chloride Anhydrous 200 200 

Magnesium Sulfate Anhydrous 97,69 97,69 

Potassium Chloride 400 400 

Sodium Acetate Anhydrous 50 50 

Sodium Bicarbonate 2200 / 

Sodium Chloride 6800 6800 

Sodium Phosphate Monobasic Anhydrous 122 122 

2`Deoxyadenosine 10 10 

2`Deoxycytidine Monohydrochloride 11,6 11,6 

2`Deoxyguanosine Monohydrate 10 10 

5-Methyl-2`-deoxycytidine hydrochloride 0,1 0,1 

Ascorbic Acid 50 50 

B-NAD 7 7 

B-NADP + Na 1 1 

Choline Chloride 0,5 0,5 

Cocarboxylase 1 1 

D-Biotin 0,01 0,01 

D-Ca Pantothenate 0,01 0,01 

Flavin Adenine Dinulceotide Disodium Salt 0,106 0,106 

Folic Acid 0,01 0,01 

Myo-Inositol 0,05 0,05 

Nicotinamide (Nicotinic acid amide) 0,025 0,025 

Nicotinic Acid 0,025 0,025 

P-Aminobenzoic Acid (PABA) 0,05 0,05 

Pyridoxal Hydrochloride 0,025 0,025 

Pyridoxine Hydrochloride 0,025 0,025 

Riboflavin 0,01 0,01 

Thiamine Hydrochloride 0,01 0,01 

Cholesterol 0,2 0,2 

Coenzyme A,Na 2,5 2,5 

D-Glucose 1000 1000 

D-Glucuronic Acid + Na 3,88 3,88 

L-Glutathione Reduced 10 10 

Phenol Red Sodium Salt 21,24 21,24 

Thymidine 10 10 

Tween 80 5 5 

Uridine-5-triphosphate + Na 1 1 




37



O. C.* 

Vitamins 


38 


Dulbecco's Modified Eagle's Medium (DMEM) 

>> DMEM High Glucose L0100 L0101 L0102 L0103 L0104 L0106 L0107 P0102 P0103 

Liquid Liquid Liquid Liquid Liquid Liquid Liquid Powder Powder 

Glutamine Stable / / / 584 / / 584 / / 

Glycine 30 30 30 30 30 30 30 30 30 


L-Cystine Dihydrochloride 62,6 62,6 62,6 62,6 62,6 62,6 62,6 62,6 62,6 

L-Glutamine / / 584 / 584 / / 584 584 

L-Histidine Monohydrochloride Monohydrate 42 42 42 42 42 42 42 42 42 

L-Isoleucine 105 105 105 105 105 105 105 105 105 

L-Leucine 105 105 105 105 105 105 105 105 105 

L-Lysine Monohydrochloride 146 146 146 146 146 146 146 146 146 

L-Methionine 30 30 30 30 30 30 30 30 30 

L-Phenylalanine 66 66 66 66 66 66 66 66 66 

L-Serine 42 42 42 42 42 42 42 42 42 

L-Threonine 95 95 95 95 95 95 95 95 95 

L-Tryptophan 16 16 16 16 16 16 16 16 16 


L-Valine 94 94 94 94 94 94 94 94 94 


Ferric Nitrate Nonahydrate 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 



Sodium Bicarbonate 3700 3700 3700 3700 3700 3700 3700 / / 


Sodium Phosphate Monobasic Anhydrous 109 109 109 109 109 109 109 109 109 

Choline Chloride 4 4 4 4 4 4 4 4 4 

D-Ca Pantothenate 4 4 4 4 4 4 4 4 4 

Folic Acid 4 4 4 4 4 4 4 4 4 

Myo-Inositol 7,2 7,2 7,2 7,2 7,2 7,2 7,2 7,2 7,2 

Nicotinamide (Nicotinic acid amide) 4 4 4 4 4 4 4 4 4 

Pyridoxal Hydrochloride 4 4 4 4 4 4 4 4 4 

Riboflavin 0,4 0,4 0,4 0,4 0,4 0,4 0,4 0,4 0,4 

Thiamine Hydrochloride 4 4 4 4 4 4 4 4 4 

D-Glucose 4500 4500 4500 4500 4500 4500 4500 4500 4500 

Hepes Free Acid 5958 / / / / / 5958 / / 

Phenol Red Sodium Salt 15,9 15,9 15,9 15,9 15,9 15,9 15,9 15,9 15,9 

Sodium Pyruvate / / / 110 110 110 / 110 / 


* Other Components

Amino Acids 


Vitamins 

O. C.* 

* Other Components 



>> DMEM Low Glucose L0060 L0064 L0065 L0066 L0067 L0070 P0061 

Liquid Liquid Liquid Liquid Liquid Liquid Powder 

mg/l mg/l mg/l mg/l mg/l mg/l mg/l 

Glutamine Stable / / / 584 / / / 

Glycine 30 30 30 30 30 30 30 

L-Arginine Monohydrochloride 84 84 84 84 84 84 84 

L-Cystine Dihydrochloride 62,6 62,6 62,6 62,6 62,6 62,6 62,6 

L-Glutamine 584 / 584 / / 584 584 

L-Histidine Monohydrochloride Monohydrate 42 42 42 42 42 42 42 

L-Isoleucine 105 105 105 105 105 105 105 

L-Leucine 105 105 105 105 105 105 105 

L-Lysine Monohydrochloride 146 146 146 146 146 146 146 

L-Methionine 30 30 30 30 30 30 30 

L-Phenylalanine 66 66 66 66 66 66 66 

L-Serine 42 42 42 42 42 42 42 

L-Threonine 95 95 95 95 95 95 95 

L-Tryptophan 16 16 16 16 16 16 16 

L-Tyrosine Disodium Salt Dihydrate 103,79 103,79 103,79 103,79 103,79 103,79 103,79 

L-Valine 94 94 94 94 94 94 94 

Calcium Chloride Anhydrous / / / / / / 200 

Calcium Chloride Dihydrate 265 265 265 265 265 265 / 

Ferric Nitrate Nonahydrate 0,1 0,1 0,1 0,1 0,1 0,1 0,1 

Magnesium Sulfate Anhydrous 97,67 97,67 97,67 97,67 97,67 97,67 97,67 

Potassium Chloride 400 400 400 400 400 400 400 

Sodium Bicarbonate 3700 3700 3700 3700 3700 / / 

Sodium Chloride 6400 6400 4400 6400 4400 6400 6400 

Sodium Phosphate Monobasic Anhydrous 109 109 109 109 109 109 109 

Choline Chloride 4 4 4 4 4 4 4 

D-Ca Pantothenate 4 4 4 4 4 4 4 

Folic Acid 4 4 4 4 4 4 4 

Myo-Inositol 7,2 7,2 7,2 7,2 7,2 7,2 7,2 

Nicotinamide (Nicotinic acid amide) 4 4 4 4 4 4 4 

Pyridoxal Hydrochloride 4 4 4 4 4 4 4 

Riboflavin 0,4 0,4 0,4 0,4 0,4 0,4 0,4 

Thiamine Hydrochloride 4 4 4 4 4 4 4 

D-Glucose 1000 1000 1000 1000 1000 1000 1000 

Hepes Free Acid / / 5958 / 5958 / / 

Phenol Red Sodium Salt 15,9 15,9 15,9 15,9 15,9 15,9 15,9 

Sodium Pyruvate 110 110 110 110 110 110 110 


39




Amino Acids 

40 

Vitamins 



>> DMEM Ham's F12 L0090 L0091 L0092 L0093 L0094 L0095 L0096 P0095 

Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Powder 


Glutamine Stable / / 365 / / / / / 

Glycine 18,75 18,75 18,75 18,75 18,75 18,75 18,75 18,75 

L-Alanine 4,45 4,45 4,45 4,45 4,45 4,45 4,45 4,45 

L-Arginine Monohydrochloride 147,5 147,5 147,5 147,5 147,5 147,5 147,5 147,5 

L-Asparagine Monohydrate 7,5 7,5 7,5 7,5 7,5 7,5 7,5 7,5 

L-Aspartic acid 6,65 6,65 6,65 6,65 6,65 6,65 6,65 6,65 

L-Cysteine Monohydrochloride Monohydrate 17,56 17,56 17,56 17,56 17,56 17,56 17,56 17,56 

L-Cystine Dihydrochloride 31,29 31,29 31,29 31,29 31,29 31,29 31,29 31,29 

L-Glutamic Acid 7,35 7,35 7,35 7,35 7,35 7,35 7,35 7,35 

L-Glutamine / / / 365 / 365 / 365 

L-Histidine Monohydrochloride Monohydrate 31,48 31,48 31,48 31,48 31,48 31,48 31,48 31,48 

L-Isoleucine 54,47 54,47 54,47 54,47 54,47 54,47 54,47 54,47 

L-Leucine 59,05 59,05 59,05 59,05 59,05 59,05 59,05 59,05 

L-Lysine Monohydrochloride 91,25 91,25 91,25 91,25 91,25 91,25 91,25 91,25 

L-Methionine 17,24 17,24 17,24 17,24 17,24 17,24 17,24 17,24 

L-Phenylalanine 35,48 35,48 35,48 35,48 35,48 35,48 35,48 35,48 

L-Proline 17,25 17,25 17,25 17,25 17,25 17,25 17,25 17,25 

L-Serine 26,25 26,25 26,25 26,25 26,25 26,25 26,25 26,25 

L-Threonine 53,45 53,45 53,45 53,45 53,45 53,45 53,45 53,45 

L-Tryptophan 9,02 9,02 9,02 9,02 9,02 9,02 9,02 9,02 

L-Tyrosine Disodium Salt Dihydrate 55,79 55,79 55,79 55,79 55,79 55,79 55,79 55,79 

L-Valine 52,85 52,85 52,85 52,85 52,85 52,85 52,85 52,85 

Calcium Chloride Dihydrate 154,5 154,5 154,5 154,5 154,5 154,5 154,5 154,5 

Cupric Sulfate Pentahydrate 0,0013 0,0013 0,0013 0,0013 0,0013 0,0013 0,0013 0,0013 

Ferric Nitrate Nonahydrate 0,05 0,05 0,05 0,05 0,05 0,05 0,05 0,05 

Ferrous Sulfate Heptahydrate 0,417 0,417 0,417 0,417 0,417 0,417 0,417 0,417 

Magnesium Chloride Hexahydrate 61,2 61,2 61,2 61,2 61,2 61,2 61,2 61,2 

Magnesium Sulfate Anhydrous 48,84 48,84 48,84 48,84 48,84 48,84 48,84 48,84 

Potassium Chloride 311,8 311,8 311,8 311,8 311,8 311,8 311,8 311,8 

Sodium Bicarbonate 2438 2438 1200 1200 1200 1200 1200 / 

Sodium Chloride 6996 6996 6996 6996 6996 6996 6996 6996 

Sodium Phosphate Dibasic Anhydrous 71,02 71,02 71,02 71,02 71,02 71,02 71,02 71,02 

Sodium Phosphate Monobasic Anhydrous 54,3 54,3 54,3 54,3 54,3 54,3 54,3 54,3 

Zinc Sulfate Heptahydrate 0,432 0,432 0,432 0,432 0,432 0,432 0,432 0,432 

Choline Chloride 8,98 8,98 8,98 8,98 8,98 8,98 8,98 8,98 

D-Biotin 0,0035 0,0035 0,0035 0,0035 0,0035 0,0035 0,0035 0,0035 

D-Ca Pantothenate 2,24 2,24 2,24 2,24 2,24 2,24 2,24 2,24 

Folic Acid 2,66 2,66 2,66 2,66 2,66 2,66 2,66 2,66 

Myo-Inositol 12,6 12,6 12,6 12,6 12,6 12,6 12,6 12,6 

Nicotinamide (Nicotinic acid amide) 2,02 2,02 2,02 2,02 2,02 2,02 2,02 2,02 

Pyridoxal Hydrochloride 2 2 2 2 2 2 2 2 

Pyridoxine Hydrochloride 0,031 0,031 0,031 0,031 0,031 0,031 0,031 0,031 

Riboflavin 0,219 0,219 0,219 0,219 0,219 0,219 0,219 0,219 

Thiamine Hydrochloride 2,17 2,17 2,17 2,17 2,17 2,17 2,17 2,17 

Vitamine B12 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 

D-Glucose 3151 / 3151 3151 3151 3151 3151 3151 

Hepes Free Acid / / 3574,5 3574,5 3574,5 5957 5957 3574,5 

Hypoxanthine 2,1 2,1 2,1 2,1 2,1 2,1 2,1 2,1 

Linoleic acid 0,042 0,042 0,042 0,042 0,042 0,042 0,042 0,042 

Phenol Red Sodium Salt 8,63 8,63 8,63 8,63 8,63 8,63 8,63 8,63 

Putrescine+2HCL 0,081 0,081 0,081 0,081 0,081 0,081 0,081 0,081 

Sodium Pyruvate 55 55 55 55 55 55 55 55 

Thioctic Acid 0,105 0,105 0,105 0,105 0,105 0,105 0,105 0,105 

Thymidine 0,365 0,365 0,365 0,365 0,365 0,365 0,365 0,365 

www.biowest.net

Amino Acids 


Vitamins 

O. C.* 


Glasgow MEM BHK 21 (GMEM) L0221 L0222 P0120 X0122 X0123 

Liquid Liquid Powder Liquid Liquid 

mg/l mg/l mg/l mg/l mg/l 

L-Arginine Monohydrochloride 42 42 42 420 420 

L-Cystine Dihydrochloride 31,29 31,29 31,29 312,9 312,9 

L-Glutamine 292 / 292 2920 / 

L-Histidine Monohydrochloride Monohydrate 21 21 21 210 210 

L-Isoleucine 52,4 52,4 52,4 524 524 

L-Leucine 52,4 52,4 52,4 524 524 

L-Lysine Monohydrochloride 73,1 73,1 73,1 731 731 

L-Methionine 15 15 15 150 150 

L-Phenylalanine 33 33 33 330 330 

L-Threonine 47,6 47,6 47,6 476 476 

L-Tryptophan 8 8 8 80 80 

L-Tyrosine Disodium Salt Dihydrate 52,19 52,19 52,19 521,9 521,9 

L-Valine 46,8 46,8 46,8 468 468 

Calcium Chloride Anhydrous / / 200 / / 

Calcium Chloride Dihydrate 265 265 / 2650 2650 

Ferric Nitrate Nonahydrate 0,1 0,1 0,1 1 1 

Magnesium Sulfate Anhydrous 97,67 97,67 97,67 976,7 976,7 

Potassium Chloride 400 400 400 4000 4000 

Sodium Bicarbonate 2750 2750 / / / 

Sodium Chloride 6400 6400 6400 64000 64000 

Sodium Phosphate Monobasic Anhydrous 107,8 107,8 / 1078 1078 

Sodium Phosphate Monobasic Dihydrate / / 124 / / 

Choline Chloride 2 2 2 20 20 

D-Ca Pantothenate 2 2 2 20 20 

Folic Acid 2 2 2 20 20 

Myo-Inositol 3,6 3,6 3,6 36 36 

Nicotinamide (Nicotinic acid amide) 2 2 2 20 20 

Pyridoxal Hydrochloride 2 2 2 20 20 

Riboflavin 0,2 0,2 0,2 2 2 

Thiamine Hydrochloride 2 2 2 20 20 

D-Glucose 4500 4500 4500 45000 45000 

Phenol Red Sodium Salt 16 16 16 160 160 




41



Vitamins 


Amino Acids 

42 


O. C.* 


Ham's F10 (F-10 Nutrient Medium) L0130 L0140 L0145 P0146 X0150 

Liquid Liquid Liquid Powder Liquid 


Glycine 7,51 7,51 7,51 7,51 75,1 

L-Alanine 9 9 9 9 90 

L-Arginine Monohydrochloride 211 211 211 211 2110 

L-Asparagine Monohydrate 15,01 15,01 15,01 15,01 150,1 

L-Aspartic acid 13,3 13,3 13,3 13,3 133 

L-Cysteine Monohydrochloride Monohydrate 35 35 35 35 350 

L-Glutamic Acid 14,7 14,7 14,7 14,7 147 

L-Glutamine 146 146 / 146 1460 

L-Histidine Monohydrochloride Monohydrate 21 21 21 21 210 

L-Isoleucine 2,6 2,6 2,6 2,6 26 

L-Leucine 13,1 13,1 13,1 13,1 131 

L-Lysine Monohydrochloride 29,3 29,3 29,3 29,3 293 

L-Methionine 4,48 4,48 4,48 4,48 44,8 

L-Phenylalanine 4,96 4,96 4,96 4,96 49,6 

L-Proline 11,5 11,5 11,5 11,5 115 

L-Serine 10,5 10,5 10,5 10,5 105 

L-Threonine 3,57 3,57 3,57 3,57 35,7 

L-Tryptophan 0,6 0,6 0,6 0,6 6 

L-Tyrosine Disodium Salt Dihydrate 2,61 2,61 2,61 2,61 26,1 

L-Valine 3,5 3,5 3,5 3,5 35 

Calcium Chloride Dihydrate 44,1 44,1 44,1 44,1 441 

Cupric Sulfate Pentahydrate 0,0025 0,0025 0,0025 0,0025 0,025 

Ferrous Sulfate Heptahydrate 0,834 0,834 0,834 0,834 8,34 

Magnesium Sulfate Anhydrous 74,64 74,64 74,64 74,64 746,4 


Potassium Phosphate Monobasic Anhydrous 83 83 83 83 830 

Sodium Bicarbonate 1200 1200 1200 / / 


Sodium Phosphate Dibasic Anhydrous 153,7 153,7 153,7 153,7 1537 

Zinc Sulfate Heptahydrate 0,0288 0,0288 0,0288 0,0288 0,288 

Choline Chloride 0,698 0,698 0,698 0,698 6,98 

D-Biotin 0,024 0,024 0,024 0,024 0,24 

D-Ca Pantothenate 0,715 0,715 0,715 0,715 7,15 

Folic Acid 1,32 1,32 1,32 1,32 13,2 

Myo-Inositol 0,541 0,541 0,541 0,541 5,41 

Nicotinamide (Nicotinic acid amide) 0,615 0,615 0,615 0,615 6,15 

Pyridoxine Hydrochloride 0,206 0,206 0,206 0,206 2,06 

Riboflavin 0,376 0,376 0,376 0,376 3,76 

Thiamine Hydrochloride 1 1 1 1 10 

Vitamine B12 1,36 1,36 1,36 1,36 13,6 

D-Glucose 1100 1100 1100 1100 11000 

Hepes Free Acid 5958 / / / / 

Hypoxanthine 4,08 4,08 4,08 4,08 40,8 

Phenol Red Sodium Salt 1,3 1,3 1,3 1,3 13 

Sodium Pyruvate 110 110 110 110 1100 

Thioctic Acid 0,21 0,21 0,21 0,21 2,1 

Thymidine 0,73 0,73 0,73 0,73 7,3 

* Other Components

Amino Acids 


Vitamins 




Ham's F12 (F-12 Nutrient Medium) L0132 L0133 L0135 L0136 P0134 X0155 

Liquid Liquid Liquid Liquid Powder Liquid 

mg/l mg/l mg/l mg/l mg/l mg/l 

Glycine 7,51 7,51 7,51 7,51 7,51 75,1 

L-Alanine 9 9 9 9 9 90 

L-Arginine Monohydrochloride 211 211 211 211 211 2110 

L-Asparagine Monohydrate 15,01 15,01 15,01 15,01 15,01 150,1 

L-Aspartic acid 13,3 13,3 13,3 13,3 13,3 133 

L-Cysteine Monohydrochloride Monohydrate 35 35 35 35 35 350 

L-Glutamic Acid 14,7 14,7 14,7 14,7 14,7 147 

L-Glutamine 146 / 146 / 146 / 

L-Histidine Monohydrochloride Monohydrate 20,96 20,96 20,96 20,96 20,96 209,6 

L-Isoleucine 3,94 3,94 3,94 3,94 3,94 39,4 

L-Leucine 13,1 13,1 13,1 13,1 13,1 131 

L-Lysine Monohydrochloride 36,5 36,5 36,5 36,5 36,5 365 

L-Methionine 4,48 4,48 4,48 4,48 4,48 44,8 

L-Phenylalanine 4,96 4,96 4,96 4,96 4,96 49,6 

L-Proline 34,5 34,5 34,5 34,5 34,5 345 

L-Serine 10,5 10,5 10,5 10,5 10,5 105 

L-Threonine 11,9 11,9 11,9 11,9 11,9 119 

L-Tryptophan 2,04 2,04 2,04 2,04 2,04 20,4 

L-Tyrosine Disodium Salt Dihydrate 7,78 7,78 7,78 7,78 7,78 77,8 

L-Valine 11,7 11,7 11,7 11,7 11,7 117 

Calcium Chloride Dihydrate 44,1 44,1 44,1 44,1 44,1 441 

Cupric Sulfate Pentahydrate 0,0025 0,0025 0,0025 0,0025 0,0025 0,025 

Ferrous Sulfate Heptahydrate 0,834 0,834 0,834 0,834 0,834 8,34 

Magnesium Chloride Hexahydrate 123 123 123 123 123 1230 

Potassium Chloride 224 224 224 224 224 2240 

Sodium Bicarbonate 1176 1176 1176 1176 / / 

Sodium Chloride 7100 7100 7599 7599 7599 75990 

Sodium Phosphate Dibasic Anhydrous 142,04 142,04 142,04 142,04 142,04 1420,4 

Zinc Sulfate Heptahydrate 0,863 0,863 0,863 0,863 0,863 8,63 

Choline Chloride 13,96 13,96 13,96 13,96 13,96 139,6 

D-Biotin 0,0073 0,0073 0,0073 0,0073 0,0073 0,073 

D-Ca Pantothenate 0,48 0,238 0,48 0,48 0,48 4,8 

Folic Acid 1,32 1,32 1,32 1,32 1,32 13,2 

Myo-Inositol 18 18 18 18 18 180 

Nicotinamide (Nicotinic acid amide) 0,037 0,037 0,037 0,037 0,037 0,37 

Pyridoxine Hydrochloride 0,062 0,062 0,062 0,062 0,062 0,62 

Riboflavin 0,038 0,038 0,038 0,038 0,038 0,38 

Thiamine Hydrochloride 0,34 0,34 0,34 0,34 0,34 3,4 

Vitamine B12 1,36 1,36 1,36 1,36 1,36 13,6 

D-Glucose 1802 1802 1802 1802 1802 18020 

Hepes Free Acid 5958 5958 / / / / 

Hypoxanthine 4,08 4,08 4,08 4,08 4,08 40,8 

Linoleic acid 0,084 0,084 0,084 0,084 0,084 0,84 

Phenol Red Sodium Salt 1,3 1,3 1,3 1,3 1,3 13 

Putrescine+2HCL 0,161 0,161 0,161 0,161 0,161 1,61 

Sodium Pyruvate 110 110 110 110 110 1100 

Thioctic Acid 0,21 0,21 0,21 0,21 0,21 2,1 

Thymidine 0,73 0,73 0,73 0,73 0,73 7,3 


43



Vitamins 


Amino Acids 

44 




Ham's F14 (F-14 Nutrient Medium) L0138 

Liquid 

mg/l 

Glycine 15 

L-Alanine 17,8 

L-Arginine Monohydrochloride 422 

L-Asparagine Anhydrous 26 

L-Aspartic acid 26,6 

L-Cysteine Monohydrochloride Monohydrate 72 

L-Glutamic Acid 29,4 

L-Histidine Monohydrochloride Monohydrate 42 

L-Isoleucine 8 

L-Leucine 26 

L-Lysine Monohydrochloride 73 

L-Methionine 8,94 

L-Phenylalanine 10 

L-Proline 69 

L-Serine 21 

L-Threonine 24 

L-Tryptophan 4 

L-Tyrosine 10,8 

L-Valine 23,4 

Calcium Chloride Dihydrate 294 

Cupric Sulfate Pentahydrate 0,0025 

Ferrous Sulfate Heptahydrate 0,834 

Magnesium Chloride Hexahydrate 172,9 

Magnesium Sulfate Heptahydrate 37 

Potassium Chloride 372,8 

Sodium Bicarbonate 1974 

Sodium Chloride 7599 

Sodium Phosphate Dibasic Heptahydrate 268 

Zinc Sulfate Heptahydrate 0,144 

Ascorbic Acid 14,97 

Choline Chloride 14 

D-Biotin 0,0073 

D-Ca Pantothenate 0,258 

Folic Acid 1,3 

Myo-Inositol 18 

Nicotinamide (Nicotinic acid amide) 0,036 

Pyridoxine Hydrochloride 0,06 

Riboflavin 0,037 

Thiamine Hydrochloride 0,3 

Vitamine B12 1,36 

Adenosine 5`-Triphosphate 1 

D-Glucose 6000 

Hypoxanthine 4,1 

Linoleic acid 0,084 

Phenol Red Sodium Salt 1,2 

Putrescine+2HCL 0,161 

Sodium Pyruvate 220 

Thioctic Acid 0,21 

Thymidine 0,73

Amino Acids 


Vitamins 

O. C.* 


Iscove's Modified Dulbecco's L0190 L0191 L0192 P0191 

Medium (IMDM) Liquid Liquid Liquid Powder 

mg/l mg/l mg/l mg/l 

Glutamine Stable / 584 / / 

Glycine 30 30 30 30 

L-Alanine 25 25 25 25 

L-Arginine Monohydrochloride 84 84 84 84 

L-Asparagine Monohydrate 28,4 28,4 28,4 28,4 

L-Aspartic acid 30 30 30 30 

L-Cystine Dihydrochloride 91,24 91,24 91,24 91,24 

L-Glutamic Acid 75 75 75 75 

L-Glutamine 584 / / 584 

L-Histidine Monohydrochloride Monohydrate 42 42 42 42 

L-Isoleucine 105 105 105 105 

L-Leucine 105 105 105 105 

L-Lysine Monohydrochloride 146 146 146 146 

L-Methionine 30 30 30 30 

L-Phenylalanine 66 66 66 66 

L-Proline 40 40 40 40 

L-Serine 42 42 42 42 

L-Threonine 95 95 95 95 

L-Tryptophan 16 16 16 16 

L-Tyrosine Disodium Salt Dihydrate 103,79 103,79 103,79 103,79 

L-Valine 94 94 94 94 

Calcium Chloride Dihydrate 219 219 219 219 

Magnesium Sulfate Anhydrous 97,67 97,67 97,67 97,67 

Potassium Chloride 330 330 330 330 

Potassium Nitrate 0,076 0,076 0,076 0,076 

Sodium Bicarbonate 3024 3024 3024 / 

Sodium Chloride 4505 4505 4505 4505 

Sodium Phosphate Monobasic Anhydrous 109 109 109 109 

Sodium Selenite 0,017 0,017 0,017 0,017 

Choline Chloride 4 4 4 4 

D-Biotin 0,013 0,013 0,013 0,013 

D-Ca Pantothenate 4 4 4 4 

Folic Acid 4 4 4 4 

Myo-Inositol 7,2 7,2 7,2 7,2 

Nicotinamide (Nicotinic acid amide) 4 4 4 4 

Pyridoxal Hydrochloride 4 4 4 4 

Riboflavin 0,4 0,4 0,4 0,4 

Thiamine Hydrochloride 4 4 4 4 

Vitamine B12 0,013 0,013 0,013 0,013 

D-Glucose 4500 4500 4500 4500 

Hepes Free Acid 5958 5958 / 5958 

Phenol Red Sodium Salt 16 15 16 16 

Sodium Pyruvate 110 110 110 110 




45





46 



Leibovitz L15 Medium L0300 P0350 

Liquid Powder 

mg/l mg/l 

DL-Alanine 450 450 

DL-Isoleucine 150 150 

DL-Phenylalanine 250 250 

DL-Threonine 600 600 

DL-Valine 200 200 

Glycine 200 200 

L-Arginine Free Base 500 500 

L-Asparagine Anhydrous 250 250 

L-Cysteine Monohydrochloride Monohydrate 157,176 157,176 

L-Glutamine 300 

L-Histidine 250 250 

L-Leucine 125 125 

L-Lysine Monohydrochloride 75 75 

L-Serine 200 200 

L-Tryptophan 20 20 

L-Tyrosine 300 300 

Calcium Chloride Dihydrate 185 185 

Magnesium Chloride Hexahydrate 200 200 

Magnesium Sulfate Anhydrous 97,67 97,67 

Potassium Chloride 400 400 

Potassium Phosphate Monobasic Anhydre 60 60 

Sodium Chloride 8000 8000 

Sodium Phosphate Dibasic Anhydrous 190 190 

Choline Chloride 1 1 

D-Ca Pantothenate 1 1 

Flavin Adenine Dinucleotide Disodium Salt 0,1 0,1 

Folic Acid 1 1 

Myo-Inositol 2 2 

Nicotinamide (Nicotinic acid amide) 1 1 

Pyridoxine Hydrochloride 1 1 

Thiamine Hydrochloride 1 1 

D-Galactose 900 900 

Phenol Red Sodium Salt 11 11 

Sodium Pyruvate 550 550 

* Other Components

Amino Acids 


Vitamins 

O.C.* 

Minimum Essential Medium (MEM) 



>> MEM w/ Earle's Salts L0415 L0416 L0430 L0440 L0444 L0445 P0450 P0451 P0452 P0460 X0311 

Liquid Liquid Liquid Liquid Liquid Liquid Powder Powder Powder Powder Liquid 

mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l 

Glutamine Stable / 292 / / / / / / / / / 

Glycine / / 7,5 / / / 7,5 / / / / 

L-Alanine / / 8,9 / / / 8,9 / / / / 

L-Arginine Monohydrochloride 126 126 126 126 126 126 126 126 126 126 1260 

L-Asparagine Monohydrate / / 15 / / / 15 / / / / 

L-Aspartic acid / / 13,3 / / / 13,3 / / / / 

L-Cystine Dihydrochloride 31,3 31,3 31,3 31,3 31,3 31,3 31,3 31,3 31,3 31,3 313 

L-Glutamic Acid / / 14,7 / / / 14,7 / / / / 

L-Glutamine 292 / / / 292 / 292 292 292 / / 

L-Histidine Monohydrochloride Monohydrate 42 42 42 42 42 42 42 42 42 42 420 

L-Isoleucine 52 52 52 52 52 52 52 52 52 52 520 

L-Leucine 52 52 52 52 52 52 52 52 52 52 520 

L-Lysine Monohydrochloride 72,5 72,5 72,5 72,5 72,5 72,5 72,5 72,5 72,5 72,5 725 

L-Methionine 15 15 15 15 15 15 15 15 15 15 150 

L-Phenylalanine 32 32 32 32 32 32 32 32 32 32 320 

L-Proline / / 11,5 / / / 11,5 / / / / 

L-Serine / / 10,5 / / / 10,5 / / / / 

L-Threonine 48 48 48 48 48 48 48 48 48 48 480 

L-Tryptophan 10 10 10 10 10 10 10 10 10 10 100 

L-Tyrosine Disodium Salt Dihydrate 51,9 51,9 51,9 51,9 51,9 51,9 51,9 51,9 51,9 36 519 

L-Valine 46 46 46 46 46 46 46 46 46 46 460 

Calcium Chloride Dihydrate 265 265 265 265 265 265 265 200 265 265 2650 

Magnesium Sulfate Anhydrous 97,67 97,67 97,67 97,67 97,67 97,67 97,67 97,67 97,67 97,67 976,7 

Potassium Chloride 400 400 400 400 400 400 400 400 400 400 4000 

Sodium Bicarbonate 2200 2200 2200 2200 2200 2200 / / / / / 

Sodium Chloride 6800 6800 6800 6800 5500 5500 6800 6800 5500 6800 68000 

Sodium Phosphate Monobasic Anhydrous 122 122 122 122 122 122 122 122 122 122 1220 

Sodium Succinate Hexahydrate / / / / / / / / / 100 / 

Succinic Acid / / / / / / / / / 75 / 

Choline Bitartrate / / / / / / / / / 1,8 / 

Choline Chloride 1 1 1 1 1 1 1 1 1 10 

D-Ca Pantothenate 1 1 1 1 1 1 1 1 1 1 10 

Folic Acid 1 1 1 1 1 1 1 1 1 1 10 

Myo-Inositol 2 2 2 2 2 2 2 2 2 2 20 

Nicotinamide (Nicotinic acid amide) 1 1 1 1 1 1 1 1 1 1 10 

Pyridoxal Hydrochloride 1 1 1 1 1 1 1 1 1 1 10 

Riboflavin 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 1 

Thiamine Hydrochloride 1 1 1 1 1 1 1 1 1 1 10 

D-Glucose Monohydrate 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 10000 

Hepes Free Acid 5958 5958 5958 

Phenol Red Sodium Salt 11 11 11 11 11 11 11 11 11 6,4 110 



47





48 



>> MEM w/ Hanks’ Salts L0465 L0470 P0515 X0321 

Liquid Liquid Powder Liquid 

mg/l mg/l mg/l mg/l 

Glycine / / 7,5 / 

L-Alanine / / 8,9 / 

L-Arginine Monohydrochloride 126 126 126 1260 

L-Asparagine Monohydrate / / 15 / 

L-Aspartic acid / / 13,3 / 

L-Cystine Dihydrochloride 31,3 31,3 31,3 313 

L-Glutamic Acid / / 14,7 / 

L-Glutamine / / 292 / 

L-Histidine Monohydrochloride Monohydrate 42 42 42 420 

L-Isoleucine 52 52 52 520 

L-Leucine 52 52 52 520 

L-Lysine Monohydrochloride 72,5 72,5 72,5 725 

L-Methionine 15 15 15 150 

L-Phenylalanine 32 32 32 320 

L-Proline / / 11,5 / 

L-Serine / / 10,5 / 

L-Threonine 48 48 48 480 

L-Tryptophan 10 10 10 100 

L-Tyrosine Disodium Salt Dihydrate 51,9 51,9 51,9 519 

L-Valine 46 46 46 460 

Calcium Chloride Dihydrate 185 185 185 1850 

Magnesium Sulfate Anhydrous 97,67 97,67 97,67 976,7 

Potassium Chloride 400 400 400 4000 

Potassium Phosphate Monobasic Anhydrous 60 60 60 600 

Sodium Bicarbonate 350 350 / / 

Sodium Chloride 8000 7500 8000 80000 

Sodium Phosphate Dibasic Anhydrous 47,88 47,88 47,88 478,8 

Choline Chloride 1 1 1 10 

D-Ca Pantothenate 1 1 1 10 

Folic Acid 1 1 1 10 

Myo-Inositol 2 2 2 20 

Nicotinamide (Nicotinic acid amide) 1 1 1 10 

Pyridoxine Hydrochloride 1 1 1 10 

Riboflavin 0,1 0,1 0,1 1 

Thiamine Hydrochloride 1 1 1 10 

D-Glucose 1000 1000 1000 10000 

Hepes Free Acid / 5958 / / 

Phenol Red Sodium Salt 11 11 11 110 

* Other Components

Amino Acids 


Vitamins 

O.C.* 


>> MEM Alpha Modification L0475 L0476 P0440 

Liquid Liquid Powder 

mg/l mg/l mg/l 

Glycine 50 50 50 

L-Alanine 25 25 25 

L-Arginine Monohydrochloride 126 126 126 

L-Asparagine Monohydrate 50 50 50 

L-Aspartic acid 30 30 30 

L-Cysteine Monohydrochloride Monohydrate 100 100 100 

L-Cystine Dihydrochloride 31,3 31,3 31,3 

L-Glutamic Acid 75 75 75 

L-Glutamine 292 / 292 

L-Histidine Monohydrochloride Monohydrate 42 42 42 

L-Isoleucine 52 52 52 

L-Leucine 52 52 52 

L-Lysine Monohydrochloride 72,5 72,5 72,5 

L-Methionine 15 15 15 

L-Phenylalanine 32 32 32 

L-Proline 40 40 40 

L-Serine 25 25 25 

L-Threonine 48 48 48 

L-Tryptophan 10 10 10 

L-Tyrosine Disodium Salt Dihydrate 51,9 51,9 51,9 

L-Valine 46 46 46 

Calcium Chloride Dihydrate 265 265 265 

Magnesium Sulfate Anhydrous 97,67 97,67 97,67 

Potassium Chloride 400 400 400 

Sodium Bicarbonate 2200 2200 / 

Sodium Chloride 6800 6800 6800 

Sodium Phosphate Monobasic Anhydrous 122 122 122 

Ascorbic Acid 50 50 50 

Choline Chloride 1 1 1 

D-Biotin 0,1 0,1 0,1 

D-Ca Pantothenate 1 1 1 

Folic Acid 1 1 1 

Myo-Inositol 2 2 2 

Nicotinamide (Nicotinic acid amide) 1 1 1 

Pyridoxal Hydrochloride 1 1 1 

Riboflavin 0,1 0,1 0,1 

Thiamine Hydrochloride 1 1 1 

Vitamine B12 1,36 1,36 1,36 

D-Glucose 1000 1000 1000 

Phenol Red Sodium Salt 11 11 11 

Sodium Pyruvate 110 110 110 

Thioctic Acid 0,2 0,2 0,2 




49

50 




Amino Acids 


Vitamins 

O.C.* 

MC Coy's 5A Medium Modified L0210 L0211 P0390 

Liquid Liquid Powder 

mg/l mg/l mg/l 

Glycine 7,51 7,51 7,51 

L-Alanine 13,36 13,36 13,36 

L-Arginine Monohydrochloride 42,14 42,14 42,14 

L-Asparagine Monohydrate 45,03 45,03 45,03 

L-Aspartic acid 19,97 19,97 19,97 

L-Cysteine Monohydrochloride Monohydrate 31,749 31,749 31,749 

L-Glutamic Acid 22,07 22,07 22,07 

L-Glutamine 219,15 / 219,15 

L-Histidine Monohydrochloride Monohydrate 20,96 20,96 20,96 

L-Hydroxy-L-Proline 19,67 19,67 19,67 

L-Isoleucine 39,36 39,36 39,36 

L-Leucine 39,36 39,36 39,36 

L-Lysine Monohydrochloride 36,54 36,54 36,54 

L-Methionine 14,92 14,92 14,92 

L-Phenylalanine 16,52 16,52 16,52 

L-Proline 17,27 17,27 17,27 

L-Serine 26,28 26,28 26,28 

L-Threonine 17,87 17,87 17,87 

L-Tryptophan 3,06 3,06 3,06 

L-Tyrosine Disodium Salt Dihydrate 26,1 26,1 26,1 

L-Valine 17,57 17,57 17,57 

Calcium Chloride Dihydrate 132 132 132 

Magnesium Sulfate Anhydrous 97,69 97,69 97,69 

Potassium Chloride 400 400 400 

Sodium Bicarbonate 2200 2200 / 

Sodium Chloride 6460 6460 6460 

Sodium Phosphate Monobasic Anhydrous 504 504 504 

Ascorbic Acid 0,5625 0,5625 0,5625 

Choline Chloride 5 5 5 

D-Biotin 0,2 0,2 0,2 

D-Ca Pantothenate 0,2 0,2 0,2 

Folic Acid 10 10 10 

Myo-Inositol 36 36 36 

Nicotinamide (Nicotinic acid amide) 0,5 0,5 0,5 

Nicotinic Acid 0,5 0,5 0,5 

P-Aminobenzoic Acid (PABA) 1 1 1 

Pyridoxal Hydrochloride 0,5 0,5 0,5 

Pyridoxine Hydrochloride 0,5 0,5 0,5 

Riboflavin 0,2 0,2 0,2 

Thiamine Hydrochloride 0,2 0,2 0,2 

Vitamine B12 2 2 2 

D-Glucose 3000 3000 3000 

L-Glutathione Reduced 0,5 0,5 0,5 

Meat peptone 600 600 600 

Phenol Red Sodium Salt 11 11 11 

* Other Components

Amino Acids 


Vitamins 




Medium 199 L0325 L0330 L0331 L0355 L0356 L0360 P0410 P0420 P0425 

Liquid Liquid Liquid Liquid Liquid Liquid Powder Powder Powder 


DL-Alanine 50 50 50 50 50 50 50 50 50 

DL-Aspartic Acid 60 60 60 60 60 60 60 60 60 

DL-Glutamic Acid 133,6 133,6 133,6 133,6 133,6 133,6 133,6 133,6 133,6 

DL-Isoleucine 40 40 40 40 40 40 40 40 40 

DL-Leucine 120 120 120 120 120 120 120 120 120 

DL-Methionine 30 30 30 30 30 30 30 30 30 

DL-Phenylalanine 50 50 50 50 50 50 50 50 50 

DL-Serine 50 50 50 50 50 50 50 50 50 

DL-Threonine 60 60 60 60 60 60 60 60 60 

DL-Tryptophan 20 20 20 20 20 20 20 20 20 

DL-Valine 50 50 50 50 50 50 50 50 50 

Glycine 50 50 50 50 50 50 50 50 50 


L-Cysteine Monohydrochloride Monohydrate 0,11 0,11 0,11 0,11 0,11 0,11 0,11 0,11 0,11 

L-Cystine Dihydrochloride 26 26 26 26 26 26 26 26 26 

L-Glutamine / 100 100 100 / 100 / 100 100 

L-Histidine Monohydrochloride Monohydrate 21,88 21,88 21,88 21,88 21,88 21,88 21,88 21,88 21,88 

L-Hydroxy-L-Proline 10 10 10 10 10 10 10 10 10 

L-Lysine Monohydrochloride 70 70 70 70 70 70 70 70 70 

L-Proline 40 40 40 40 40 40 40 40 40 



Ferric Nitrate Nonahydrate 0,72 0,72 0,72 0,72 0,72 0,72 0,72 0,72 0,72 



Potassium Phosphate Monobasic Anhydrous 60 60 60 / / / 60 / / 

Sodium Acetate Anhydrous 50 50 50 50 50 50 50 50 50 

Sodium Bicarbonate 350 350 350 1250 2200 2200 / / / 


Sodium Phosphate Dibasic Anhydrous 47,88 47,88 47,88 / / / 47,88 / / 

Sodium Phosphate Monobasic Anhydrous / / / 122 122 122 / 122 122 

Ascorbic Acid 0,0566 0,0566 0,0566 0,0566 0,0566 0,0566 0,0566 0,0566 0,0566 

Choline Chloride 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 

DL-alpha-Tocopherol Phosphate Disodium Salt 0,01 0,01 0,01 0,01 0,01 0,01 0,01 0,01 0,01 

D-Biotin 0,01 0,01 0,01 0,01 0,01 0,01 0,01 0,01 0,01 

D-Ca Pantothenate 0,01 0,01 0,01 0,01 0,01 0,01 0,01 0,01 0,01 

Ergocalciferol 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 

Folic Acid 0,01 0,01 0,01 0,01 0,01 0,01 0,01 0,01 0,01 

Menadione Sodium Bisulfite 0,016 0,016 0,016 0,016 0,016 0,016 0,016 0,016 0,016 

Myo-Inositol 0,05 0,05 0,05 0,05 0,05 0,05 0,05 0,05 0,05 

Nicotinamide (Nicotinic acid amide) 0,025 0,025 0,025 0,025 0,025 0,025 0,025 0,025 0,025 

Nicotinic Acid 0,025 0,025 0,025 0,025 0,025 0,025 0,025 0,025 0,025 

P-Aminobenzoic Acid (PABA) 0,05 0,05 0,05 0,05 0,05 0,05 0,05 0,05 0,05 

Pyridoxal Hydrochloride 0,025 0,025 0,025 0,025 0,025 0,025 0,025 0,025 0,025 

Pyridoxine Hydrochloride 0,025 0,025 0,025 0,025 0,025 0,025 0,025 0,025 0,025 

Riboflavin 0,01 0,01 0,01 0,01 0,01 0,01 0,01 0,01 0,01 

Thiamine Hydrochloride 0,01 0,01 0,01 0,01 0,01 0,01 0,01 0,01 0,01 

Vitamine A Acetate 0,14 0,14 0,14 0,14 0,14 0,14 0,14 0,14 0,14 

2 Deoxy-D-ribose 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 

Adenine Sulfate 10 10 10 10 10 10 10 10 10 

Adenosine 5' Monophosphate 0,2385 0,2385 0,2385 0,2385 0,2385 / / 0,2385 / 

Adenosine Monophosphate Na Salt,2H2O / / / / / 0,2385 0,2385 / 0,2385 

Adenosine-5-Triphosphate x 2Na 1 1 1 1 1 1 1 1 1 

Cholesterol 0,2 0,2 0,2 0,2 0,2 0,2 0,2 0,2 0,2 

D-Glucose 1000 1000 1000 1000 1000 1000 1000 1000 1000 

Guanine 0,3 0,3 0,3 0,3 0,3 / / 0,3 / 

Guanine + HCl / / / / / 0,3 0,3 / 0,3 

Hepes Free Acid / / 5958 / / 5958 / / 5958 

Hypoxanthine 0,3 0,3 0,3 0,3 0,3 0,3 0,3 0,3 0,3 

L-Glutathione Reduced 0,05 0,05 0,05 0,05 0,05 0,05 0,05 0,05 0,05 

Phenol Red Sodium Salt 21,3 21,3 21,3 21,3 21,3 21,3 21,3 21,3 21,3 

Ribose 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 

Thymine 0,3 0,3 0,3 0,3 0,3 0,3 0,3 0,3 0,3 

Tween 80 20 20 20 20 20 20 20 20 20 

Uracil 0,3 0,3 0,3 0,3 0,3 0,3 0,3 0,3 0,3 

Xanthine + Na 0,344 0,344 0,344 0,344 0,344 0,344 0,344 0,344 0,344 


51





52 


RPMI 1640 Medium L0490 L0492 L0495 L0496 L0498 L0500 L0501 L0503 L0504 L0505 

Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid 

mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l 

Glutamine Stable / / / 300 300 / / / / / 

Glycine 10 10 10 10 10 10 10 10 10 10 

L-Arginine Free Base 200 200 200 200 200 200 200 200 200 200 

L-Asparagine Anhydrous 50 50 50 50 50 50 50 50 50 50 

L-Aspartic acid 20 20 20 20 20 20 20 20 20 20 

L-Cystine Dihydrochloride 65,2 65,2 65,2 65,2 65,2 65,2 65,2 65,2 65,2 65,2 

L-Glutamic Acid 20 20 20 20 20 20 20 20 20 20 

L-Glutamine / / 300 / / 300 / / 300 / 

L-Histidine 15 15 15 15 15 15 15 15 15 15 

L-Hydroxy-L-Proline 20 20 20 20 20 20 20 20 20 20 

L-Isoleucine 50 50 50 50 50 50 50 50 50 50 

L-Leucine 50 50 50 50 50 50 50 50 50 50 

L-Lysine Monohydrochloride 40 40 40 40 40 40 40 40 40 40 

L-Methionine 15 15 15 15 15 15 15 15 15 15 

L-Phenylalanine 15 15 15 15 15 15 15 15 15 15 

L-Proline 20 20 20 20 20 20 20 20 20 20 

L-Serine 30 30 30 30 30 30 30 30 30 30 

L-Threonine 20 20 20 20 20 20 20 20 20 20 

L-Tryptophan 5 5 5 5 5 5 5 5 5 5 

L-Tyrosine Disodium Salt Dihydrate 28,83 28,83 28,83 28,83 28,83 28,83 28,83 28,83 28,83 28,83 

L-Valine 20 20 20 20 20 20 20 20 20 20 

Calcium Nitrate Tetrahydrate 100 100 100 100 100 100 100 100 100 100 

Magnesium Sulfate Anhydrous 48,84 48,84 48,84 48,84 48,84 48,84 48,84 48,84 48,84 48,84 

Potassium Chloride 400 400 400 400 400 400 400 400 400 400 

Sodium Bicarbonate 2000 1000 2000 2000 2000 2000 2000 2000 / 2000 

Sodium Chloride 6000 6400 6000 6000 6000 6000 6000 6000 6000 6000 

Sodium Phosphate Dibasic Anhydrous 800 800 800 800 800 800 800 800 800 800 

Choline Chloride 3 3 3 3 3 3 3 3 3 3 

D-Biotin 0,2 0,2 0,2 0,2 0,2 0,2 0,2 0,2 0,2 0,2 

D-Ca Pantothenate 0,25 0,25 0,25 0,25 0,25 0,25 0,25 0,25 0,25 0,25 

Folic Acid 1 1 1 1 1 1 1 / 1 1 

Myo-Inositol 35 35 35 35 35 35 35 35 35 35 

Nicotinamide (Nicotinic acid amide) 1 1 1 1 1 1 1 1 1 1 

P-Aminobenzoic Acid (PABA) 1 1 1 1 1 1 1 1 1 1 

Pyridoxine Hydrochloride 1 1 1 1 1 1 1 1 1 1 

Riboflavin 0,2 0,2 0,2 0,2 0,2 0,2 0,2 0,2 0,2 0,2 

Thiamine Hydrochloride 1 1 1 1 1 1 1 1 1 1 

Vitamine B12 0,005 0,005 0,005 0,005 0,005 0,005 0,005 0,005 0,005 0,005 

D-Glucose 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 

Hepes Free Acid 5960 4770 5960 5960 / / / / / / 

L-Glutathione Reduced 1 1 1 1 1 1 1 1 1 1 

Phenol Red Sodium Salt 5,3 5,3 5,3 5,3 5,3 5,3 5,3 5,3 5,3 / 


* Other Components

Amino Acids 


Vitamins 

O.C. 




RPMI 1640 Medium P0860 P0870 P0871 P0875 P0876 P0877 P0880 P0883 

Powder Powder Powder Powder Powder Powder Powder Powder 

mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l 

Glutamine Stable / / / / / 300 / / 

Glycine 10 10 10 10 10 10 10 10 

L-Arginine Free Base 200 200 200 200 / / 200 200 

L-Arginine Monohydrochloride / / / / 241,9 241,9 / / 

L-Asparagine Anhydrous 50 50 50 50 50 50 50 50 

L-Aspartic acid 20 20 20 20 20 20 20 20 

L-Cystine / / / / 50 50 / / 

L-Cystine Dihydrochloride 65,2 65,2 65,2 65,2 / / 65,2 65,2 

L-Glutamic Acid 20 20 20 20 20 20 20 20 

L-Glutamine 300 / / / 300 / 300 300 

L-Histidine 15 15 15 15 15 15 15 15 

L-Hydroxy-L-Proline 20 20 20 20 20 20 20 20 

L-Isoleucine 50 50 50 50 50 50 50 50 

L-Leucine 50 50 50 50 50 50 50 50 

L-Lysine Monohydrochloride 40 40 40 40 40 40 40 40 

L-Methionine 15 15 15 15 15 15 15 15 

L-Phenylalanine 15 15 15 15 15 15 15 15 

L-Proline 20 20 20 20 20 20 20 20 

L-Serine 30 30 30 30 30 30 30 30 

L-Threonine 20 20 20 20 20 20 20 20 

L-Tryptophan 5 5 5 5 5 5 5 5 

L-Tyrosine / / / / 20 20 / / 

L-Tyrosine Disodium Salt Dihydrate 28,83 28,83 28,83 28,83 / / 28,83 28,83 

L-Valine 20 20 20 20 20 20 20 20 

Calcium Nitrate Tetrahydrate 100 100 100 100 100 100 100 100 

Magnesium Sulfate Anhydrous 48,84 48,84 48,84 48,84 / / 48,84 48,84 

Magnesium Sulfate Heptahydrate / / / / 100 100 / / 

Potassium Chloride 400 400 400 400 400 400 400 400 

Sodium Bicarbonate / / / / / 2000 / / 

Sodium Chloride 6000 6000 6000 6000 5500 5500 6000 6000 

Sodium Phosphate Dibasic Anhydrous 800 800 800 800 800 800 800 800 

Choline Chloride 3 3 3 3 3 3 3 3 

D-Biotin 0,2 0,2 0,2 0,2 0,2 0,2 0,2 0,2 

D-Ca Pantothenate 0,25 0,25 0,25 0,25 0,24 0,24 0,25 0,25 

Folic Acid 1 1 1 1 1 1 1 1 

Myo-Inositol 35 35 35 35 35 35 35 35 

Nicotinamide (Nicotinic acid amide) 1 1 1 1 1 1 1 1 

P-Aminobenzoic Acid (PABA) 1 1 1 1 1 1 1 1 

Pyridoxine Hydrochloride 1 1 1 1 1 1 1 1 

Riboflavin 0,2 0,2 0,2 0,2 0,2 0,2 0,2 0,2 

Thiamine Hydrochloride 1 1 1 1 1 1 1 1 

Vitamine B12 0,005 0,005 0,005 0,005 0,005 0,005 0,005 0,005 

D-Glucose 2000 2000 2000 2000 2000 2000 2000 / 

Hepes Free Acid / / / 5960 5958 5958 / / 

L-Glutathione Reduced 1 1 1 1 1 1 1 1 

Phenol Red Sodium Salt 5,3 5,3 / 5,3 / / / 5,3 


53



O.C.* 


54 



Schneider's Drosophila Medium L0207 

Liquid 

mg/l 

Beta-Alanine 500 

Glycine 250 

L-Arginine Free Base 600 

L-Aspartic acid 400 

L-Cysteine Monohydrochloride Monohydrate 78,588 

L-Cystine Dihydrochloride 26,732 

L-Glutamic Acid 800 

L-Glutamine 1800 

L-Histidine 400 

L-Isoleucine 150 

L-Leucine 150 

L-Lysine Monohydrochloride 1650 

L-Methionine 150 

L-Proline 1700 

L-Serine 250 

L-Threonine 350 

L-Tryptophan 100 

L-Tyrosine Disodium Salt Dihydrate 720,199 

L-Valine 300 

Calcium Chloride Anhydrous 600 

Magnesium Sulfate Anhydrous 1807,221 

Potassium Chloride 1600 

Potassium Phosphate Monobasic Anhydrous 450 

Sodium Bicarbonate 400 


Sodium Phosphate Dibasic Anhydrous 700 

Succinic Acid 60 

Alpha-Ketoglutaric Acid 350 

D(+)-Trehalose Dihydrate 2000 


Fumaric Acid 60 

L-Malic Acid 600 

Yeast Extract 2000 

* Other Components


Preparation Instructions 

>> Preparation Instructions for Powder Media 

Powdered Media are extremely hygroscopic and should be protected from 

atmospherical moisture. The entire content of each package should be closed 

immediately after opening. Preparing a concentrated solution of medium is not 

recommended as precipitates may form. 

Supplements can be added prior to filtration or introduced aseptically to sterile 

medium. The nature of the supplements may effect storage conditions and shelf 

life of the medium. 

1. Measure out 90% of initial required volume of water. Water temperature 

should be at 15 - 20 °C. 

2. While gently stirring the water, add the powdered medium. Stirr until dissolved. 

Do not heat. 

3. Rinse original package with small amount of water to remove all traces of 

powder. Add to solution in step 2. 

4. Supplementation of the medium after your needs. 

5. While stirring, adjust the pH of the medium to 0,1 – 0,3 pH units below the 

desired pH since it may rise during filtration. The use of 1N HCl or 1N NaOH is 

recommended. 

6. Add additional water to bring the solution to final volume. 

7. Sterilize immediately by filtration using a membrane with a porosity of 0,22 

microns or less. 

8. Aseptically dispense medium into sterile container. 

>> Product Storage 

Store the dry powdered medium at +2 / +8°C under dry conditions and liquid 

medium at +2 / +8°C in the dark. 

Deterioration of the powdered medium may be recognized by any or all of the following 

: 

color range 

granulation / clumping 

insolubility 

Deterioration of the following into liquid media : 

pH change 

precipitate or particulate matter 

throughout the solution 

cloudy appearance 

color change 

The nature of supplements added may affect storage conditions and shelf life 

of the medium. Product label bears expiration date. 


55

56 

Custom Made Formulation 

For your personalised cell culture media composition, choose in the list of substances, give 

us the components needed and the corresponding quantities. We will produce it to your 

requirements. 

AMINO ACIDS 

CAS N° Component Formulation Quantity 

107-95-9 Beta-Alanine C3H7NO2 . . . . . . . . . . . . . . . . 

302-72-7 DL-Alanine C3H7NO2 . . . . . . . . . . . . . . . . 

617-45-8 DL-Aspartic Acid C4H7NO4 . . . . . . . . . . . . . . . . 

617-65-2 DL-Glutamic Acid C5H9NO4 . . . . . . . . . . . . . . . . 

443-79-8 DL-Isoleucine C6 H13 NO2 . . . . . . . . . . . . . . . . 

328-39-2 DL-Leucine C6 H13 NO2 . . . . . . . . . . . . . . . . 

59-51-8 DL-Methionine C5H11NO2S . . . . . . . . . . . . . . . . 

150-30-1 DL-Phenylalanine C9H11NO2 . . . . . . . . . . . . . . . . 

302-84-1 DL-Serine C3H7NO3 . . . . . . . . . . . . . . . . 

80-68-2 DL-Threonine C4H9NO3 . . . . . . . . . . . . . . . . 

54-12-6 DL-Tryptophan C11H12N2O2 . . . . . . . . . . . . . . . . 

516-06-3 DL-Valine C5H11NO2 . . . . . . . . . . . . . . . . 

640-68-6 D-Valine C5H11NO2 . . . . . . . . . . . . . . . . 

121574-43-4 Glutamine Stable C10H17N3O5 . . . . . . . . . . . . . . . . 

56-40-6 Glycine C2H5NO2 . . . . . . . . . . . . . . . . 

56-41-7 L-Alanine C3H7NO2 . . . . . . . . . . . . . . . . 

74-79-3 L-Arginine Free Base C6H14N4O2 . . . . . . . . . . . . . . . . 

1119-34-2 L-Arginine Monohydrochloride C6H14N4O2.HCL . . . . . . . . . . . . . . . . 

70-47-3 L-Asparagine Anhydrous C4H8N2O3 . . . . . . . . . . . . . . . . 

5794-13-8 L-Asparagine Monohydrate C4H8N2O3.H2O . . . . . . . . . . . . . . . . 

56-84-8 L-Aspartic acid C4H7NO4 . . . . . . . . . . . . . . . . 

52-90-4 L-Cysteine Free Base C3H7NO2S . . . . . . . . . . . . . . . . 

7048-04-6 L-Cysteine Monohydrochloride Monohydrate C3H7NO2S.HCl.H2O . . . . . . . . . . . . . . . . 

56-89-3 L-Cystine C6H12N2O4S2 . . . . . . . . . . . . . . . . 

30925-07-6 L-Cystine Dihydrochloride C6H12N2O4S2.2HCL . . . . . . . . . . . . . . . . 

56-86-0 L-Glutamic Acid C5H9NO4 . . . . . . . . . . . . . . . . 

56-85-9 L-Glutamine C5H10N2O3 . . . . . . . . . . . . . . . . 

71-00-1 L-Histidine C6H9N3O2 . . . . . . . . . . . . . . . . 

5934-29-2 L-Histidine Monohydrochloride Monohydrate C6H9N3O2.HCL.H2O . . . . . . . . . . . . . . . . 

51-35-4 L-Hydroxy-L-Proline C5H9NO3 . . . . . . . . . . . . . . . . 

73-32-5 L-Isoleucine C6H13NO2 . . . . . . . . . . . . . . . . 

61-90-5 L-Leucine C6H13NO2 . . . . . . . . . . . . . . . . 

657-27-2 L-Lysine Monohydrochloride C6H14N2O2.HCL . . . . . . . . . . . . . . . . 

63-68-3 L-Methionine C5H11NO2S . . . . . . . . . . . . . . . . 

3184-13-2 L-Ornithine Monohydrochloride H12N2C5O2xHCl . . . . . . . . . . . . . . . . 

63-91-2 L-Phenylalanine C9H11NO2 . . . . . . . . . . . . . . . . 

147-85-3 L-Proline C5H9NO2 . . . . . . . . . . . . . . . . 

56-45-1 L-Serine C3H7NO3 . . . . . . . . . . . . . . . . 

107-35-7 L-Taurine C2H7NO3S . . . . . . . . . . . . . . . . 

72-19-5 L-Threonine C4H9NO3 . . . . . . . . . . . . . . . . 

73-22-3 L-Tryptophan C11H12N2O2 . . . . . . . . . . . . . . . . 

60-18-4 L-Tyrosine C9H11NO3 . . . . . . . . . . . . . . . . 

69847-45-6 L-Tyrosine Disodium Salt Dihydrate C9H11N03+2Na+2H2O . . . . . . . . . . . . . . . . 

72-18-4 L-Valine C5H11NO2 . . . . . . . . . . . . . . . . 


www.biowest.net

INORGANIC SALTS 




12125-02-9 Ammonium Chloride NH4Cl . . . . . . . . . . . . . . . . 

7803-55-6 Ammonium Metavanadate NH4VO3 . . . . . . . . . . . . . . . . 

12054-85-2 Ammonium molybdate tetrahydrate H24MO7N6O244H2O . . . . . . . . . . . . . . . . 

6484-52-2 Ammonium Nitrate NH4NO3H4N2O3 . . . . . . . . . . . . . . . . 

7783-20-2 Ammonium Sulfate (NH4)2SO4 H8N2O4S . . . . . . . . . . . . . . . . 

0043-52-4 Calcium Chloride Anhydrous CaCl2 . . . . . . . . . . . . . . . . 

10035-04-8 Calcium Chloride Dihydrate CaCL2.2H2O . . . . . . . . . . . . . . . . 

7774-34-7 Calcium Chloride Hexahydrate CaCl2.6H2O . . . . . . . . . . . . . . . . 

13477-34-4 Calcium Nitrate Tetrahydrate Ca(NO3)2 x.4H2O . . . . . . . . . . . . . . . . 

77-92-9 Citric acid free acid C6H8O7 . . . . . . . . . . . . . . . . 

6132-04-3 Citric acid Trisodium salt dihydrate C6H5Na3O7 x 2H2O . . . . . . . . . . . . . . . . 

7646-79-9 Cobalt Chloride Anhydrous CoCl2 . . . . . . . . . . . . . . . . 

7791-13-1 Cobalt Chloride Hexahydrate CoCl2 x 6 H2O . . . . . . . . . . . . . . . . 

10125-13-0 Cupric Chloride Dihydrate CuCl2-2H2O . . . . . . . . . . . . . . . . 

7758-99-8 Cupric Sulfate Pentahydrate CuSO4.5H2O . . . . . . . . . . . . . . . . 

50978-11-5 Diatrizoic Acid Dihydrate C11H9I3N2O4.2H2O . . . . . . . . . . . . . . . . 

60-00-4 EDTA C10H16N2O8 . . . . . . . . . . . . . . . . 

6381-92-6 EDTA Disodium Salt Dihydrate C10H14N2Na2O8.2H2O . . . . . . . . . . . . . . . . 

10378-23-1 EDTA Tetrasodium Salt Dihydrate C10H12N2Na4O8. 2H2O . . . . . . . . . . . . . . . . 

7783-83-7 Ferric Ammonium Sulfate Dodecahydrate Fe NH4SO4 .12 H2O . . . . . . . . . . . . . . . . 

10025-77-1 Ferric Chloride Hexahydrate FeCl3.6H2O . . . . . . . . . . . . . . . . 

7782-61-8 Ferric Nitrate Nonahydrate FeN3O9.9H2O . . . . . . . . . . . . . . . . 

7782-63-0 Ferrous Sulfate Heptahydrate FeO4S.7H2O . . . . . . . . . . . . . . . . 

007786-30-3 Magnesium Chloride Anhydrous MgCL2 . . . . . . . . . . . . . . . . 

7791-18-6 Magnesium Chloride Hexahydrate MgCL2.6H2O . . . . . . . . . . . . . . . . 

7487-88-9 Magnesium Sulfate Anhydrous MgSO4 . . . . . . . . . . . . . . . . 

10034-99-8 Magnesium Sulfate Heptahydrate MgSO4.7H2O . . . . . . . . . . . . . . . . 

10034-96-5 Manganese Sulfate Monohydrate MnSO4xH2O . . . . . . . . . . . . . . . . 

10102-40-6 Molybdic Acid Sodium Salt Dihydrate NaMoO4 . . . . . . . . . . . . . . . . 

7791-20-0 Nickel chloride Hexahydrate NiCl2x6H2O . . . . . . . . . . . . . . . . 

7447-40-7 Potassium Chloride KCL . . . . . . . . . . . . . . . . 

7681-11-0 Potassium Iodide KI . . . . . . . . . . . . . . . . 

7757-79-1 Potassium Nitrate KNO3 . . . . . . . . . . . . . . . . 

7758-09-0 Potassium nitrite KNO2 . . . . . . . . . . . . . . . . 

7778-77-0 Potassium Phosphate Monobasic Anhydre KH2PO4 . . . . . . . . . . . . . . . . 

127-09-3 Sodium Acetate Anhydrous C2H3O2Na . . . . . . . . . . . . . . . . 

144-55-8 Sodium Bicarbonate NaHCO3 . . . . . . . . . . . . . . . . 

497-19-8 Sodium Carbonate Na CO3 . . . . . . . . . . . . . . . . 

7647-14-5 Sodium Chloride NaCl . . . . . . . . . . . . . . . . 

302-95-4 Sodium Deoxycholate C24H39O4Na . . . . . . . . . . . . . . . . 

13517-24-3 Sodium Metasilicate Nonahydrate Na2SiO3 . 9H2O . . . . . . . . . . . . . . . . 

7558-79-4 Sodium Phosphate Dibasic Anhydrous Na2HPO4 . . . . . . . . . . . . . . . . 

7782-85-6 Sodium Phosphate Dibasic Heptahydrate Na2HPO4 x 7H2O . . . . . . . . . . . . . . . . 

7558-80-7 Sodium Phosphate Monobasic Anhydrous NaH2PO4 . . . . . . . . . . . . . . . . 

13472-35-0 Sodium Phosphate Monobasic Dihydrate NaH2PO4.2H2O . . . . . . . . . . . . . . . . 

10049-21-5 Sodium Phosphate Monobasic Monohydrate NaH2PO4xH2O . . . . . . . . . . . . . . . . 

10102-18-8 Sodium Selenite Na2SeO3 . . . . . . . . . . . . . . . . 

6106-21-4 Sodium Succinate Hexahydrate C4H4Na2O4.6H2O . . . . . . . . . . . . . . . . 

10025-69-1 Stannous Chloride dihydrate SnCl2 x 2H2O . . . . . . . . . . . . . . . . 

110-15-6 Succinic Acid C4H6O4 . . . . . . . . . . . . . . . . 

7772-99-8 Tin chloride SnCl2 . . . . . . . . . . . . . . . . 

7446-20-0 Zinc Sulfate Heptahydrate ZnSO4.7H2O . . . . . . . . . . . . . . . . 


57

58 


VITAMINS 


958-09-8 2'Deoxyadenosine C10H13N5O3 . . . . . . . . . . . . . . . . 

3992-42-5 2'Deoxycytidine Monohydrochloride C9H13N3O4+HCl . . . . . . . . . . . . . . . . 

3416-05-5 3'-Deoxythymidine C10H14N2O4 . . . . . . . . . . . . . . . . 

838-07-3 5-Methyl-2'-deoxycytidine hydrochloride C10H15N3O4 . HCl . . . . . . . . . . . . . . . . 

50-81-7 Ascorbic Acid C6H8O6 . . . . . . . . . . . . . . . . 

134-03-2 Ascorbic Acid,Sodium Salt C6H7NaO6 . . . . . . . . . . . . . . . . 

53-84-9 B-NAD C12H27N7O14P2 . . . . . . . . . . . . . . . . 

1184-16-3 B-NADP + Na C21H27N7O17P3Na . . . . . . . . . . . . . . . . 

87-67-2 Choline Bitartrate C5H14NO+C4H5O6 . . . . . . . . . . . . . . . . 

67-48-1 Choline Chloride C5H14CLNO . . . . . . . . . . . . . . . . 

154-87-0 Cocarboxylase C12H19N4O7SCl . . . . . . . . . . . . . . . . 

58-85-5 D-Biotin C10H16N2O3S . . . . . . . . . . . . . . . . 

137-08-6 D-Ca Pantothenate C18H32CaN2O10 . . . . . . . . . . . . . . . . 

60934-46-5 DL-Alpha-Tocopherol Phosphate Disodium Salt C29H49O5PNa2 . . . . . . . . . . . . . . . . 

60-32-2 E-Amino-N Coproïc Acid . . . . . . . . . . . . . . . . 

50-14-6 Ergocalciferol C28H44O . . . . . . . . . . . . . . . . 

146-14-5 Flavin Adenine Dinucleotide Disodium Salt C27H31N9O15P2Na2 . . . . . . . . . . . . . . . . 

59-30-3 Folic Acid C19H19N7O6 . . . . . . . . . . . . . . . . 

1492-18-8 Folinic Acid C20H21N7O7Ca . . . . . . . . . . . . . . . . 

130-37-0 Menadione Sodium Bisulfite C11H8O2.NaHSO3 . . . . . . . . . . . . . . . . 

87-89-8 Myo-Inositol C6H12O6 . . . . . . . . . . . . . . . . 

98-92-0 Nicotinamide (Nicotinic acid amide) C6H6N2O . . . . . . . . . . . . . . . . 

59-67-6 Nicotinic Acid C6H5NO2 . . . . . . . . . . . . . . . . 

150-13-0 P-Aminobenzoic Acid (PABA) C7H7NO2 . . . . . . . . . . . . . . . . 

65-22-5 Pyridoxal Hydrochloride C8H9NO3+HCl . . . . . . . . . . . . . . . . 

58-56-0 Pyridoxine Hydrochloride "C8H11NO3;HCL " . . . . . . . . . . . . . . . . 

83-88-5 Riboflavin C17H20N4O6 . . . . . . . . . . . . . . . . 

67-03-8 Thiamine Hydrochloride "C12H17CLN4OS;HCL" . . . . . . . . . . . . . . . . 

127-47-9 Vitamine A Acetate C22H32O2 . . . . . . . . . . . . . . . . 

68-19-9 Vitamine B12 C63H88CoN14O14P . . . . . . . . . . . . . . . . 

10191-41-0 Vitamine E C29H50O2 . . . . . . . . . . . . . . . . 

OTHERS COMPONENTS 


533-67-5 2 Deoxy-D-ribose C5H10O4 . . . . . . . . . . . . . . . . 

58366-64-6 5-Methylcytosine Hydrochloride C5H7N3O . . . . . . . . . . . . . . . . 

321-30-2 Adenine Hemisulfate C5H5N5 + 1/2 H2SO4 . . . . . . . . . . . . . . . . 

6055-72-7 Adenine Monohydrochloride C5H5N5.HCL . . . . . . . . . . . . . . . . 

6509-19-9 Adenine Sulphate C5H5N5+H2SO4 . . . . . . . . . . . . . . . . 

58-61-7 Adenosine C10H13N5O4 . . . . . . . . . . . . . . . . 

18422-05-4 Adenosine 5'Monophosphate C10H14N5O7P . . . . . . . . . . . . . . . . 

56-65-5 Adenosine 5'-Triphosphate C10H16N5O13P3 . . . . . . . . . . . . . . . . 

4578-31-8 Adenosine Monophosphate Na Salt.2H2O C10H12N5Na2O7P . . . . . . . . . . . . . . . . 

149022-20-8 Adenosine-5'-Monophosphate x Na C10H14N5O7PxNa . . . . . . . . . . . . . . . . 

51963-61-2 Adenosine-5-Triphosphate x 2Na C10H14N5O13P3Na2 . . . . . . . . . . . . . . . . 

328-50-7 Alpha-Ketoglutaric Acid C5H6O5 . . . . . . . . . . . . . . . . 

1397-89-3 Amphotericin B C47H73NO17 . . . . . . . . . . . . . . . . 

Bovine Insuline . . . . . . . . . . . . . . . . 


www.biowest.net




9048-46-8 Bovine Serum Albumin . . . . . . . . . . . . . . . . 

57-88-5 Cholesterol C27H46O . . . . . . . . . . . . . . . . 

55672-92-9 Coenzyme A.Na C21H36N7O16P3S . . . . . . . . . . . . . . . . 

65-46-3 Cytidine C9H13N3O5 . . . . . . . . . . . . . . . . 

6138-23-4 D(+)-Trehalose C12H22O11 . . . . . . . . . . . . . . . . 

477-30-5 Demecolcine C21H25NO5 . . . . . . . . . . . . . . . . 

59.23.4 D-Galactose C6H12O6 . . . . . . . . . . . . . . . . 

66-84-2 D-Glucosamine X HCl C6H13NO5.HCL . . . . . . . . . . . . . . . . 

50-99-7 D-Glucose Anhydrous C6H12O6 . . . . . . . . . . . . . . . . 

5996-10-1 D-Glucose Monohydrate C6H12O6 x H2O . . . . . . . . . . . . . . . . 

32449-92-6 D-Glucuronolactone C6H8O6 . . . . . . . . . . . . . . . . 

50-69-1 D-Ribose C5H10 O5 . . . . . . . . . . . . . . . . 

Fructose . . . . . . . . . . . . . . . . 

110-17-8 Fumaric Acid C4H4O4 . . . . . . . . . . . . . . . . 

108321-42-2 G-418 C20H40O10N4x2H2SO4 . . . . . . . . . . . . . . . . 

1405-41-0 Gentamicin Sulfate C19-21H39-43N5O7 x 2.5H2SO4 . . . . . . . . . 

73-40-5 Guanine C5H5N5O . . . . . . . . . . . . . . . . 

635-39-2 Guanine + HCl C5H5N5O.HCl . . . . . . . . . . . . . . . . 

118-00-3 Guanosine C10H13N5O5 . . . . . . . . . . . . . . . . 

7365-45-9 Hepes Free Acid C8H18N2O4S . . . . . . . . . . . . . . . . 

75277-39-3 Hepes Sodium Salt C8H17N2O4SNa . . . . . . . . . . . . . . . . 

68-94-0 Hypoxanthine C5H4N4O . . . . . . . . . . . . . . . . 

25389-94-0 Kanamycin Sulfate C18H36N4O11 H2SO4 . . . . . . . . . . . . . . . . 

68458-87-7 Lactalbumin Hydrolysate Edamin S . . . . . . . . . . . . . . . . 

1492-24-6 L-Amino-n-Butyric Acid C4H9NO2 . . . . . . . . . . . . . . . . 

70-18-8 L-Glutathione Reduced C10H17N3O6S . . . . . . . . . . . . . . . . 

60-33-3 Linoleic acid C18H32O2 . . . . . . . . . . . . . . . . 

97-67-6 L-Malic Acid C4H6O5 . . . . . . . . . . . . . . . . 

Meat peptone . . . . . . . . . . . . . . . . 

1132-61-2 MOPS C7H15NO4S . . . . . . . . . . . . . . . . 

57-10-3 Palmitinsaeure/Palmitic Acid . . . . . . . . . . . . . . . . 

69-57-8 Penicillin G Sodium Salt . . . . . . . . . . . . . . . . 

143-74-8 Phenol red free acid . . . . . . . . . . . . . . . . 

34487-61-1 Phenol Red Sodium Salt C19 H13 NaO5S . . . . . . . . . . . . . . . . 

1405-20-5 Polymyxin B Sulfat . . . . . . . . . . . . . . . . 

26873-85-8 Polysaccharose 400 . . . . . . . . . . . . . . . . 

Protein TH (Toxin Hand) . . . . . . . . . . . . . . . . 

333-93-7 Putrescine+2HCL C4H12N2 x 2HCl . . . . . . . . . . . . . . . . 

113-24-6 Sodium Pyruvate C3H3O3Na . . . . . . . . . . . . . . . . 

Soy Peptone . . . . . . . . . . . . . . . . 

3810-74-0 Streptomycin Sulfate C42H84N14O36S3 . . . . . . . . . . . . . . . . 

57-50-1 Sucrose C12H22O11 . . . . . . . . . . . . . . . . 

1077-28-7 Thioctic Acid C8H14O2S2 . . . . . . . . . . . . . . . . 

50-89-5 Thymidine C10H14N2O5 . . . . . . . . . . . . . . . . 

65-71-4 Thymine C5H6N2O2 . . . . . . . . . . . . . . . . 

9002-07-7 Trypsin 1:250 . . . . . . . . . . . . . . . . 

Tryptose . . . . . . . . . . . . . . . . 

Tryptose phosphate broth . . . . . . . . . . . . . . . . 

9005-65-6 Tween 80 . . . . . . . . . . . . . . . . 

9005-65-6 Tween 81 . . . . . . . . . . . . . . . . 

66-22-8 Uracil C4H4N2O2 . . . . . . . . . . . . . . . . 

19817-92-6 Uridine-5-triphosphate + Na . . . . . . . . . . . . . . . . 

69-89-6 Xanthine C5H3N4O2 . . . . . . . . . . . . . . . . 

1196-43-6 Xanthine + Na C5H3N4O2Na . . . . . . . . . . . . . . . . 

Yeast Extract 


59

60 

Table of Addition 

of L-Glutamine 

Table of Addition of L-Glutamine 

Cat N° Description P1012 X0550 

g/ L ml / L 

L0042 BME w/ Earle's Salts w/o L-Glutamine 0,292 10 

L0044 BME w/ Earle's Salts w/o L-Glutamine w/ 25 mM Hepes 0,292 10 

L0046 BME w/ Hanks' Salts w/o L-Glutamine 0,292 10 

L0053 CMRL 1066 w/o L-Glutamine 0,1 3,4 

L0064 DMEM Low Glucose w/o L-Glutamine w/ Sodium Pyruvate 0,584 20 

L0067 DMEM Low Glucose w/o L-Glutamine w/ Sodium Pyruvate 0,584 20 

w/ 25 mM Hepes 

L0090 DMEM F12 w/o L-Glutamine w/o Hepes 0,365 12,5 

L0091 DMEM F12 w/o L-Glutamine w/o Hepes w/o Glucose 0,365 12,5 

L0094 DMEM F12 w/o L-Glutamine w/ 15 mM Hepes 0,365 12,5 

L0096 DMEM F12 w/o L-Glutamine w/ 25 mM Hepes 0,365 12,5 

L0100 DMEM High Glucose w/o L-Glutamine w/ 25mM Hepes 0,584 20 

L0101 DMEM High Glucose w/o L-Glutamine w/o Sodium Pyruvate 0,584 20 

L0106 DMEM High Glucose w/o L-Glutamine w/ Sodium Pyruvate 0,584 20 

L0133 Ham's F12 w/o L-Glutamine w/ 25 mM Hepes 0,146 5 

L0136 Ham's F12 w/o L-Glutamine 0,146 5 

L0138 Ham's F14 w/ 6 g/L Glucose w/ 1mg/L ATP 0,292 10 

L0145 Ham's F10 w/o L-Glutamine 0,146 5 

L0192 IMDM w/o L- Glutamine w/o Hepes 0,584 20 


L0211 McCoy's 5A w/o L-Glutamine 0,2192 7,5 

L0222 Glasgow MEM BHK 21 w/o L-Glutamine 0,292 10 

w/o Tryptose Phosphate Broth 

L0300 Leibovitz L-15 Medium w/o L-Glutamine 0,3 10,25 

L0325 Medium 199 w/ Hanks' Salts w/o L-Glutamine 0,1 3,4 


addition of 

L-Glutamine powder 

addition of 

L-Glutamine 100X, 

200mM solution


Table of Addition 

of L-Glutamine 

add° of 

L-Glutamine 

powder 


g/ L ml / L 

L0356 Medium 199 w/ Earles' Salts w/o L-Glutamine 0,1 3,4 

L0430 MEM w/ Earle's Salts w/o L-Glutamine w/NEAA 0,292 10 

L0440 MEM w/ Earle's Salts w/o L-Glutamine 0,292 10 

L0445 MEM w/ Earle's Salts w/o L-Glutamine w/ 25mM Hepes 0,292 10 

L0465 MEM w/ Hanks' Salts w/o L-Glutamine 0,292 10 

L0470 MEM w/ Hanks' Salts w/o L-Glutamine w/ 25mM Hepes 0,292 10 

L0476 MEM Alpha w/o L-Glutamine w/o Ribunocleosides 0,292 10 

w/o Deoxyribonucleosides 

L0490 RPMI 1640 w/o L-Glutamine w/ 25 mM Hepes 0,3 10,25 

L0492 RPMI 1640 Dutch Modification w/o L-Glutamine 0,3 10,25 

w/ 1g/l Sodium Bicarbonate w/ 20mM Hepes 

L0501 RPMI 1640 w/o L-Glutamine 0,3 10,25 

L0503 RPMI 1640 w/o L-Glutamine w/o Folic Acid 0,3 10,25 

L0505 RPMI 1640 w/o L-Glutamine w/o Phenol Red 0,3 10,25 

P0410 Medium 199 w/ Hanks' Salts w/o L-Glutamine 0,1 3,4 

w/o Sodium Bicarbonate 

P0460 MEM Autoclave w/ Earle's Salts w/o L-Glutamine 0,292 10 


P0870 RPMI 1640 w/o L-Glutamine w/o Sodium Bicarbonate 0,3 10,25 


w/o Phenol Red 


w/ 25mM Hepes 

X0026 BME 10X w/ Hanks' Salts w/o L-Glutamine 0,292 10 

w/o Sodium Bicarbonate For 1X For 1X 

X0042 BME 10X w/ Earle's Salts w/o L-Glutamine 0,292 10 


X0123 Glasgow MEM BHK 21 10X w/o L-Glutamine 0,292 10 

w/o Tryptose Phosphate Broth For 1X For 1X 

add° 

L-Glutamine 

100X, 

200mM solution 


61

62 

Table of addition 

of Sodium Bicarbonate 


g/ L ml / L 

X0155 Ham's F12 10X w/o L-Glutamine w/o Sodium Bicarbonate 0,146 5 

For 1X for 1X 

X0311 MEM 10X w/ Earle's Salts w/o L-Glutamine 0,292 10 


X0321 MEM 10X w/ Hank's Salts w/o L-Glutamine 0,292 10 


Table of Addition of Sodium Bicarbonate 

Cat N° Description g/ L ml / L 

L0070 DMEM Low Glucose w/ L-Glutamine w/o Sodium Bicarbonate 3,7 49,3 

w/ Sodium Pyruvate 

L0504 RPMI 1640 w/ L-Glutamine w/o Sodium Bicarbonate 2 26,7 

L0605 Hanks' Balanced Salt Solution w/o Calcium w/o Magnesium 0,35 4,7 

w/o Sodium Bicarbonate w/o Phenol Red 

L0608 Hanks' Balanced Salt Solution w/ Calcium w/ Magnesium 0,35 4,7 

w/o Sodium Bicarbonate w/ Phenol Red 

L0609 Hanks' Balanced Salt Solution w/o Calcium w/o Magnesium 0,35 4,7 


P0030 BME w/ Earle's Salts w/ L-Glutamine w/o Sodium Bicarbonate 2,2 29,3 

P0031 BME w/ Hanks' Salts w/ L-Glutamine w/o Sodium Bicarbonate 0,35 4,7 

P0058 CMRL 1066 w/ L-Glutamine w/o Sodium Bicarbonate 2,2 29,3 

P0061 DMEM Low Glucose w/ L-Glutamine w/o Sodium Bicarbonate 3,7 49,3 



P0095 DMEM F12 w/ L-Glutamine w/o Sodium Bicarbonate 1,2 16 


P0102 DMEM High Glucose w/ L-Glutamine w/o Sodium Bicarbonate 3,7 49,3 


P0103 DMEM High Glucose w/ L-Glutamine w/o Sodium Bicarbonate 3,7 49,3 

w/o Sodium Pyruvate 


addition of 

L-Glutamine powder 

addition of Sodium 

Bicarbonate powder 

P2060 

addition of 

L-Glutamine 100X, 

200mM solution 

addition of 

Sodium bicarbonate 

7,5% solution L0680




add° of Sodium 

Bicarbonate 

powder P2060 


P0120 Glasgow MEM BHK 21 w/ L-Glutamine w/o Sodium Bicarbonate 2,75 36,7 


P0134 Ham's F12 w/ L-Glutamine w/o Sodium Bicarbonate 1,176 15,7 

P0146 Ham's F10 w/ L-Glutamine w/o Sodium Bicarbonate 1,2 16 

P0153 Hanks' Balanced Salts w/o Calcium w/o Magnesium 0,35 4,7 


P0154 Hanks' Balanced Salts w/ Calcium w/ Magnesium 0,35 4,7 


P0191 IMDM w/ L- Glutamine w/o Sodium Bicarbonate 3,024 40,3 


P0390 Mc Coy's 5A w/ L-Glutamine w/o Sodium Bicarbonate 2,2 29,3 

P0410 Medium 199 w/ Hanks' Salts w/o L-Glutamine 0,35 4,7 


P0420 Medium 199 w/ Earle's Salts w/ L-Glutamine 2,2 29,3 


P0425 Medium 199 w/ Earle’s Salts w/ L-Glutamine w/o Sodium 2,2 29,3 

Bicarbonate w/ 25mM Hepes 

P0440 MEM Alpha Modification w/ Earle's Salts w/ L-Glutamine 2,2 29,3 


P0450 MEM w/ Earle's Salts w/ L-Glutamine w/ NEAA 2,2 29,3 


P0451 MEM w/ Earle's Salts w/ L-Glutamine w/o NEAA 2,2 29,3 


P0452 MEM w/ Earle's Salts w/ L-Glutamine 2,2 29,3 

w/o Sodium Bicarbonate w/ 25mM Hepes 

P0460 MEM Autoclave w/ Earle's Salts w/o L-Glutamine 2,2 29,3 


P0515 MEM w/ Hanks' Salts w/ L-Glutamine w/ NEAA 0,35 4,7 


P0860 RPMI 1640 w/ L-Glutamine w/o Sodium Bicarbonate 2 26,7 



bicarbonate 

7,5% solution 

L0680 

63

64 






Bicarbonate 

powder P2060 


P0870 RPMI 1640 w/o L-Glutamine w/o Sodium Bicarbonate 2 26,7 



w/ 25mM Hepes 


w/25 mM Hepes w/o Phenol Red 


w/o Phenol Red 


w/o Glucose 

X0026 BME 10X w/ Hanks' Salts w/o L-Glutamine 0,35 For 1X 4,7 For 1X 


X0042 BME 10X w/ Earle's Salts w/o L-Glutamine 2,2 For 1X 29,3 For 1X 


X0112 Earle's Balanced Salt Solution 10X w/ Calcium w/ Magnesium 2,2 For 1X 29,3 For 1X 


X0113 Earle's Balanced Salt Solution 10x w/o Calcium w/o Magnesium 2,2 For 1X 29,3 For 1X 


X0122 Glasgow MEM BHK 21 10X w/ L-Glutamine 2,75 For 1X 36,7 for 1X 


X0123 Glasgow MEM BHK 21 10X w/o L-Glutamine 2,75 For 1X 36,7 for 1X 


X0150 Ham's F10 10X w/ L-Glutamine w/o Sodium Bicarbonate 1,2 For 1X 16 For 1X 

X0155 Ham's F12 w/o L-Glutamine w/o Sodium Bicarbonate 1,176 For 1X 15,7 For 1X 

X0311 MEM 10X w/ Earle's Salts w/o L-Glutamine 2,2 For 1X 29,3 For 1X 


X0321 MEM 10X w/ Hank's Salts w/o L-Glutamine 0,35 For 1X 4,7 For 1X 


X0507 Hanks' Balanced Salt Solution 10X w/o Calcium w/o Magnesium 0,35 For 1X 4,7 For 1X 


X0509 Hanks' Balanced Salt Solution 10X w/ Calcium w/ Magnesium 0,35 For 1X 4,7 For 1X 


X0513 Hanks' Balanced Salt Solution 10X w/o Calcium w/o Magnesium 0,35 For 1X 4,7 For 1X 



bicarbonate 

7,5% solution 

L0680

Salt Solutions & Salts/ Buffers 

Presentation 

Product List 

Cat N° Description Unit/Size 

Liquid 

L0615 Dulbecco's Phosphate Buffered Saline w/o Calcium w/o Magnesium 100 ml 

State : Liquid Storage : +20° C Life : 48 months 500 ml 

L0625 Dulbecco's Phosphate Buffered Saline w/ Calcium w/ Magnesium 100 ml 


Powder 

The irrigating buffers and salt solutions are sterile physiological balanced solutions 

intended for use in the maintenance of mammalian cells where a chemically defined, 

balanced salt solution provides an environment that will maintain the structural and 

physiological integrity of cells in vitro. They were used for the first steps for 

preparing parts of organs and during the dissociation process or for isolation of cell 

suspensions. Further applications are the intermediate steps for cultivation of cells 

like washing, centrifugation, suspending and counting and also many analytical 

methods or biochemical treatments. Principally these solutions should be used to 

keep the cells only for minutes or maximal some hours into suspension. 

These buffers and solutions are no cell culture media. 

They are composed of a Phosphate buffer system, sodium chloride to adjust the 

osmolarity and perhaps sugar for a short time nutrition and stabilisation of morphology. 

For applications where Ca2+ and Mg2+ ions interfere with enzyme activity e.g. 

Trypsin, use the modified buffers w/o Calcium w/o Magnesium. 

Dulbecco's Phosphate Buffered Saline DPBS >> See composition page 71 

P0750 Dulbecco's Phosphate Buffered Saline w/o Calcium w/o Magnesium For 1 L 

State : Powder Storage : +20° C Life : 48 months For 5 L 

For 10 L 


X0515 Dulbecco's Phosphate Buffered Saline 10X w/o Calcium w/o Magnesium 100 ml 


X0520 Dulbecco's Phosphate Buffered Saline 10X w/ Calcium w/ Magnesium 100 ml 



65

66 

Hanks' Balanced Salts Solution HBSS 

Salt Solutions Salt Solutions & Salts / Buffers 


Product List 

Earle's Balanced Salts Solution EBSS >> See composition page 71 


Liquid 

L0600 Earle's Balanced Salt Solution w/o Calcium w/o Magnesium w/o Phenol Red 100 ml 


L0601 Earle's Balanced Salt Solution w/o Calcium w/o Magnesium 100 ml 


L0602 Earle's Balanced Salt Solution w/ Calcium w/ Magnesium 100 ml 



X0112 Earle's Balanced Salt Solution 10X w/ Calcium w/ Magnesium w/o Sodium 

Bicarbonate 100 ml 


X0113 Earle's Balanced Salt Solution 10x w/o Calcium w/o Magnesium w/o Sodium 

Bicarbonate 100 ml 



Liquid 


L0605 Hanks' Balanced Salts Solution w/o Calcium 100 ml 

w/o Magnesium w/o Sodium Bicarbonate w/o Phenol Red 500 ml 

State : Liquid Storage : +20° C Life : 48 months 

L0606 Hanks' Balanced Salts Solution w/ Calcium w/ Magnesium 100 ml 

w/ Sodium Bicarbonate w/ Phenol Red 500 ml 



w/o Magnesium w/ Sodium Bicarbonate w/o Phenol Red 500 ml 



w/o Sodium Bicarbonate w/ Phenol Red 500 ml 


See page 59-63 for addition of Sodium Bicarbonate


L0609 Hanks' Balanced Salts Solution w/o Calcium w/o Magnesium 100 ml 

w/o Sodium Bicarbonate w/ Phenol Red 500 ml 



w/o Magnesium w/ Sodium Bicarbonate w/ Phenol Red 500 ml 



w/ Sodium Bicarbonate w/o Phenol Red 500 ml 


Powder 

P0153 Hanks' Balanced Salts w/o Calcium w/o Magnesium For 1 L 

w/o Sodium Bicarbonate w/o Phenol Red For 5 L 


P0154 Hanks' Balanced Salts w/ Calcium w/ Magnesium For 1 L 




See page 59-63 for addition of Sodium Bicarbonate 


Product List 

X0507 Hanks' Balanced Salts Solution 10X w/o Calcium 100 ml 

w/o Magnesium w/o Sodium Bicarbonate w/o Phenol Red 500 ml 


X0509 Hanks' Balanced Salts Solution 10X w/ Calcium 100 ml 

w/ Magnesium w/o Sodium Bicarbonate w/ Phenol Red 500 ml 



w/o Magnesium w/ Sodium Bicarbonate w/o Phenol Red 500 ml 



w/o Magnesium w/o Sodium Bicarbonate w/ Phenol Red 500 ml 



67

Salt Solutions 

68 

Salt Solutions & Salts / Buffers 

Hepes 


Liquid 

L0180 Hepes Buffer 1 M 100 ml 


Powder 

P5455 Hepes, cell culture tested 250 g 

State : Crystalline Storage : +20° C Life : 24 months 500 g 

1000 g 

Other Salt Solutions 


Liquid 

L0636 Alsever's Solution 100 ml 


L0640 NaCl Salt Solution 0.85 % 100 ml 


L0641 NaCl Salt solution 0.9% (non sterile) 5 L 


L0643 Potassium Chloride 0.075 M 100 ml 


L0680 Sodium Bicarbonate 7.5 % 100 ml 


L0642 Sodium Pyruvate 100 mM 100 ml 


L0630 Versen 100 ml 



Product List 

>> See composition page 72

Salts 

Cat N° Description 

>> Unit / Size of Salts on request 


Product List 

P2092 Calcium Chloride Anhydrous 

State : Powder Storage : +20° C Life : 36 months CAS N°10043-52-4 

P2090 Calcium Chloride Dihydrate 

State : Powder Storage : +20° C Life : 36 months CAS N° 10035-04-8 

P2099 Calcium Nitrate Tetrahydrate 


P2006 Cupric Sulfate Pentahydrate 

State : Crystalline Storage : +20° C Life : 36 months CAS N° 7758-99-8 

P1099 EDTA-Ethylenediaminetetraacetic acid 


P2010 Ferric Nitrate Nonahydrate 


P2015 Ferrous Sulfate Heptahydrate 


P2020 Magnesium Chloride Anhydrous 


P2021 Magnesium Chloride Hexahydrate 


P2025 Magnesium Sulfate Anhydrous 


P2032 Manganese sulfate monohydrate 


P2034 Nickel chloride Hexahydrate 


P2035 Potassium Chloride 


P2040 Potassium Dihydrogen Phosphate Anhydrous 


P2151 Potassium Hydroxide (pellets) 


P2140 Potassium Tetraborate Tetrahydrate 



69


70 



P2060 Sodium Bicarbonate, cell culture tested 


P2066 Sodium Chloride 


P2065 Sodium Chloride (for dilution 8.5 g/l) 


P2064 Sodium Chloride (for dilution 9 g/l) 


P2110 Sodium Hydroxide (pellets) 


P2067 Sodium Molybdate Dihydrate 


P2076 Sodium Phosphate Dibasic Anhydrous 


P2079 Sodium Phosphate Dibasic Heptahydrate 


P2069 Sodium Phosphate Monobasic Anhydrous 


P2073 Sodium Phosphate Monobasic Dihydrate 


P2071 Sodium Phosphate Monobasic Monohydrate 


P5810 Sodium Pyruvate 

State : Crystalline Storage: +4 ° C Life : 36 months CAS N° 113-24-6 

P5911 Thioctic Acid 


P2130 Tripotassium Citrate Anhydrous 


P2120 Trisodium Citric Acid 


P2088 Zinc Sulfate Heptahydrate 



Product List 

>> Unit / Size of Salts on request




Inorganic Salts O.C.* 

O.C.* 


O.C.* 


Product List 

Dulbecco's Phosphate Buffered Saline L0615 L0625 P0750 X0515 X0520 

DPBS Liquid Liquid Powder Liquid Liquid 


Calcium Chloride Dihydrate / 133 / / 1330 

Magnesium Chloride Hexahydrate / 100 / / 1000 


Potassium Phosphate Monobasic Anhydrous 200 200 200 2000 2000 


Sodium Phosphate Dibasic Anhydrous 1150 1150 1150 11500 11500 

Earle's Balanced Salts Solution L0600 L0601 L0602 X0112 X0113 

EBSS Liquid Liquid Liquid Liquid Liquid 


Calcium Chloride Dihydrate / / 265 2650 / 

Magnesium Sulfate Anhydrous / / 97,67 976,7 / 


Sodium Bicarbonate 2200 2200 2200 / / 


Sodium Phosphate Monobasic Anhydrous 122 122 122 1220 1220 

D-Glucose 1000 1000 1000 10000 10000 

Phenol Red Sodium Salt / 11 11 110 110 

Hank's Balanced Salts Solution L0605 L0606 L0607 L0608 L0609 L0611 L0612 

HBSS Liquid Liquid Liquid Liquid Liquid Liquid Liquid 

mg/l mg/l mg/l mg/l mg/l mg/l mg/l 

Calcium Chloride Dihydrate / 185 / 185 / / 185 

Magnesium Sulfate Anhydrous / 97,67 / 97,67 / / 97,67 

Potassium Chloride 400 400 400 400 400 400 400 

Potassium Phosphate Monobasic Anhydrous 60 60 60 60 60 60 60 

Sodium Bicarbonate / 350 350 / / 350 350 

Sodium Chloride 8000 8000 8000 8000 8000 8000 8000 

Sodium Phosphate Dibasic Anhydrous 47,88 47,88 47,88 47,88 47,88 47,68 47,88 

D-Glucose 1000 1000 1000 1000 1000 1000 1000 

Phenol Red Sodium Salt / 11 / 11 11 11 / 

Hank's Balanced Salts Solution P0153 P0154 X0507 X0509 X0510 X0513 

HBSS Powder Powder Liquid Liquid Liquid Liquid 

mg/l mg/l mg/l mg/l mg/l mg/l 


Calcium Chloride Dihydrate / 185 / 1850 / / 

Magnesium Sulfate Anhydrous / 97,68 / 976,7 / / 

Potassium Chloride 400 400 4000 4000 4000 4000 

Potassium Phosphate Monobasic Anhydrous 60 60 600 600 600 600 

Sodium Bicarbonate / / / / 3500 / 

Sodium Chloride 8000 8000 80000 80000 80000 80000 

Sodium Phosphate Dibasic Anhydrous 47,88 47,88 476,8 478,8 476,8 476,8 

D-Glucose 1000 1000 10000 10000 10000 10000 

Phenol Red Sodium Salt / 11 / 110 / 110 


71


72 



O.C.* 


O.C.* 


Product List 

Alsever's Solution L0636 

Liquid 

mg/l 

Citric acid free acid 550 

Citric acid Trisodium salt dihydrate 8000 



Versen L0630 

Liquid 

mg/l 

EDTA Disodium Salt Dihydrate 292 


Potassium Phosphate Monobasic Anhydrous 190 



D-Glucose 198 

* Other Components

Antibiotics 

Presentation 

The use of antibiotics is a helpful tool in the cell culture field or where fluids 

have to be conserved and prevented from bacterial contamination. Most of 

the antibiotics suppress the growth of micro-organisms by blocking an 

anabolic pathway. NANOMYCOPULITINE is a new development : this toxin 

actively kills the bacteria in all three stages of development without 

interfering with the eukaryotic metabolism. 


73

Antibiotics 

74 

Antibiotics 

Product List 


Liquid 

L0009 Amphotericin B 100 ml 

State : Frozen Storage : -20° C Life : 24 months N°CAS : 1397-89-3 

L0010 Antibiotic-Antimycotic 100X 20 ml 

State: Frozen Storage : -20° C Life : 24 months 100 ml 

R : 42 S : 23-45 

L0011 Gentamicin Sulfate 10mg/ml 10ml 

State : Frozen Storage : -20° C Life : 24 months N° CAS : 1405-41-0 100 ml 

R : 42/43 S : 23 - 36/37 - 45 

L0012 Gentamicin Sulfate 50mg/ml 10ml 

State: Frozen Storage : -20° C Life : 24 months N° Cas : 1405-41-0 100 ml 

R : 42/43 S : 23 - 36/37 - 45 

L0013 Glutamine-Gentamicin 10ml 


L0014 Glutamine-Penicillin-Streptomycin 100X 100ml 

State: Frozen Storage : -20° C Life : 24 months 

L0015 G-418 Solution 10ml 

State : Frozen Storage : -20° C Life : 24 months N°CAS : 108321-42-2 100 ml 

S : 23-24/25 

L-X16 Nanomycopulitine Concentrat 20X 10ml 

State: Frozen Storage : -20° C Life : 24 months 100ml 

L0018 Penicillin-streptomycin 20ml 

State : Frozen Storage : -20° C Life : 24 months 100ml 

L0022 Penicillin-streptomycin Solution 100X 20ml 

State : Frozen Storage : -20° C Life : 24 months 100ml 

L0017 Polymyxin B Sulfate 100ml 

State : Frozen Storage : -20° C Life : 24 months N° CAS : 1405-20-05 

www.biowest.net

Antibiotics 

Product List 

Composition 


Powder 

P4030 Amphotericin B on request 

State : Powder Storage : +2/+8 °C Life: 24 months N°CAS 1397-89-3 

R : 36/37/38 S : 26-36 

P0016 Ciprofloxacin Hydrochloride on request 


P0017 G-418 Sulfate on request 


S : 22- 24/25 

P4020 Gentamicin Sulfate on request 

State : Powder Storage : +2/+8 °C Life: 48 months N° CAS 1405-41-0 

R : 42/43 S : 22-36/37-45 

P0019 Hygromycin B on request 

State : Powder Storage : +2/+8 °C Life: 36 months N° CAS 31282-04-9 

R : 22-23-41-42/43 S : 22-26-28-45-36/37/39 

P0018 Penicillin G Sodium Salt,~1.650 units per mg on request 


R : 42/43 S : 22-36/37-45 

P0025 Streptomycin Sulfate on request 

State : Powder Storage : +2/+8 °C Life: 24 months 

R : 22 

ANTIBIOTICS L0010 L0018 L0022 L0013 L0014 

Liquid Liquid Liquid Liquid Liquid 

Amino Acids 

L-Glutamine / / / 29.200 mg/l 29.200 mg/l 

Inorganic Salts / / 

Sodium Chloride 8.500 mg/l 8.500 mg/l 8.500 mg/l 9.000 mg/l / 


Amphotericin B 25 mg/l / / / / 

Gentamicin Sulfate / / / 5.000 mg/l / 

Penicillin G Sodium Salt 10.000.000 U/l 100.000 U/l 10.000.000 U/l / 10.000 U/l 

Streptomycin Sulfate 10.000 mg/l 100 mg/l 10.000 mg/l / 10.000 mg/l 


75

Antibiotics 

76 

Antibiotics 

Composition 


Gentamycin Sulfate 10 mg/ml L0011 

Liquid 


Gentamicin Sulfate 10.000 mg/l 

Gentamycin Sulfate 10 mg/ml L0012 

Liquid 


Gentamicin Sulfate 50.000 mg/l 

G-418 Solution L0015 

Liquid 


G-418 50.000 mg/l 

Nanomycopulitine Concentrat 20X L-X16 

Liquid 


Ciprofloxacin Hydrochloride 400 mg/l 

Polymyxin B Sulfat L0017 

Liquid 


Polymyxin B Sulfat 10.000 U/ml 

Amphotericin B L0009 

Liquid 


Sodium Deoxycholate 205 mg/l 


Amphotericin B 250 mg/l

Cell culture 

Reagents 

Cell Culture Reagents 

Summary 

Page 

Trypsin . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 

MEM 50X / 100X concentrate . . . . . . . . . . 79 

BME 100X concentrate . . . . . . . . . . . . . . . . 79 

Lymphosep . . . . . . . . . . . . . . . . . . . . . . . . . 79 

Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . 80 

Colcemid . . . . . . . . . . . . . . . . . . . . . . . . . . 81 

Cell Culture Water . . . . . . . . . . . . . . . . . . . . 81 

Phenol Red . . . . . . . . . . . . . . . . . . . . . . . . . 81 

Trypan Blue . . . . . . . . . . . . . . . . . . . . . . . . 82 

Amino Acids . . . . . . . . . . . . . . . . . . . . 82 - 84 

Vitamins . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 


77



78 

Product List 

Trypsin >> See composition page 86 

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation 

and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration 

of trypsin necessary to dislodge the cells from their substrate depends on the 

sensitivity of the cells. 


Liquid 

L0909 Trypsin 0.25 % in PBS w/o Calcium w/o Magnesium w/ Phenol red 100 ml 


L0910 Trypsin 0.25 % in PBS w/o Calcium w/o Magnesium 100 ml 


L0930 Trypsin-EDTA 1X in PBS w/o Calcium w/o Magnesium w/ Phenol Red 100 ml 


L0931 Trypsin 0.25% - EDTA in HBSS w/o Calcium w/o Magnesium w/ Phenol Red 100 ml 


L0932 Trypsin 0.25% - EDTA 0.02% in HBSS w/o Calcium w/o Magnesium w/ Phenol Red 100 ml 


L0940 Trypsin-EDTA 1X in PBS w/o Calcium w/o Magnesium w/o Phenol Red 100 ml 


Powder 

P0940 Trypsin - EDTA 1X Lyophilised w/ Sodium Chloride 1 g 

State : Powder Storage : +2/+8 °C Life : 24 months 100 g 

P5957 Trypsin 1:250 powder (porcine) 1 g 

State : Powder Storage : +2/+8 °C Life : 48 months 100 g 


X0915 Trypsin 2.5 % in PBS w/o Calcium w/o Magnesium w/o Phenol Red 100 ml 


X0920 Trypsin 2.5 % in HBSS w/o Calcium w/o Magnesium w/o Phenol Red 100 ml 


X0930 Trypsin-EDTA 10X 100 ml 


X0960 Trypsin 2.5% 10X in Puck`s Salts 100 ml 


www.biowest.net


Product List 

MEM 50X / 100X and BME 100X concentrate Amino Acids, Vitamins, NEAA 


The solution of amino acids, vitamins or non essential amino acids concentrated can be 

added to your basal medium (the final concentration of the MEM or BME must be 1X) to 

enrich it and then this medium is used as a classical MEM or BME. 


50X / 100x concentration 

X0552 BME Amino Acids 100X w/o L-Glutamine 100ml 


X0554 MEM Amino Acids 50X w/o L-Glutamine 100ml 


X0555 BME Vitamins 100X w/o L-Glutamine 100ml 


X0556 MEM Vitamins 100X w/o L-Glutamine 100ml 


X0557 MEM Non Essential Amino Acids 100X w/o L-Glutamine 100ml 


Lymphosep is designed for the simple, rapid isolation of lymphocytes from whole blood 

that has been diluted and treated with anti-coagulant or defibrinating agent. 


Liquid 

Lymphosep 


L0560 Lymphosep, Lymphozyte Separation Media 100 ml 



79



80 

Product List 


Powder 

Glutamine 

L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells 

grown in culture. It is a crucial component of many cell culture media and serves as a major 

energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a 

frozen solution. However, in liquid media or stock solutions, L-Glutamine can degrade 

relatively rapidly. L-Glutamine is also more labile in cell culture solution than other amino acids. 

L-Glutamine degradation results in a build up of ammonia which could have a deleterious 

effect on some culturing systems. 

Dipeptide derivatives of L-Glutamine prevent the intramolecular cyclization reaction 

associated with solutions of L-Glutamine. These derivatives are therefore stable in solution 

and allow the formulation of cell culture media containing L-Glutamine that may be stored 

at 4°C for extended periods. Solutions containing these derivatives can be even autoclaved 

without appreciable degradation of the product (30 minutes at 121°C results in


Product List 


Liquid 

L0970 Cell Culture Water Pyrogen free 500 ml 



Liquid 

Cell Culture Water 

Colcemid 

Colcemid arrested the division of cells in mitosis. It prevents the formation of the spindle 

apparatus responsible for cell division, thereby permitting an accumulation of metaphases. 

L0040 Colcemid 10 µg / ml in PBS 10 ml 


50 ml 

Phenol Red 

Phenol red is used as a pH indicator. The sodium salt of phenol red is used widely in 

culture media to identify changes from neutral to acidic pH values. A solution of phenol red 

will have a yellow color at a pH of 6.4 or below and a red color at a pH of 8.2 and above. 


Liquid 

L0541 Phenol Red Solution 0.5 % 100 ml 


Powder 


P5648 Phenol Red Sodium Salt 



81



82 

Product List 

Amino Acids 

Trypan Blue 

Trypan Blue is a stain used to distinguish viable from non viable cells. 


Liquid 

L0990 Trypan blue 0.5 % Solution (non sterile) 100 ml 


Powder 

P0935 Trypan blue (Direct blue 14) 


Amino Acids 


P1001 L-Alanine 


P1002 L-Arginine, Free base 


P1003 L-Arginine Hydrochloride 


P1004 L-Asparagine Anhydrous 


P1005 L-Asparagine Monohydrate 


P1006 L-Aspartic Acid 


P1007 L-Cysteine, Free Base 


P1008 L-Cysteine Hydrochloride Monohydrate 



>> Unit / Size of amino acids on request


>> Unit / Size of amino acids on request 


Amino Acids 

P1009 L-Cystine 


P1010 L-Cystine Dihydrochloride 


P1011 L-Glutamic Acid 


P1012 L-Glutamine 


P1013 Glycine 


P1014 L-Histidine Base 

State : Crystalline Storage : +20° C Life : 36 months CAS N° 71-00- 

P1015 L-Histidine Hydrochloride Monohydrate 


P1016 L-Isoleucine 

State : Crystalline Storage : +20° C Life : 36 months CAS N° 73-32- 

P1017 L-Leucine 


P1018 L-Lysine Monohydrochloride 


P1019 L-Methionine 


P1020 L-Phenylalanine 


P1021 L-Proline 


P1022 L-Hydroxyproline 


P1023 L-Serine 


P1024 L-Threonine 



83



84 

Amino Acids 


P1025 L-Tryptophane 


P1026 L-Tyrosine 


P1027 L-Tyrosine Disodium Salt Dihydrate 


P1028 L-Valine 


P1031 L-Alanyl-L-Glutamine, stable Glutamine 


P1030 Lactalbumine, hydrolysate 

State : Powder Storage : +2/+8 °C Life : 36 months CAS N° 68458-87-7 

P1040 L-Ornithin HCl 


P1060 DL-Alanine 


P1065 DL-Leucine 

State : Powder Storage : +20° C Life : 48 months CAS N° 328-39- 

P1070 DL-Phenylalanine 


P1085 DL-Trytophan 


P1360 D-Phenylalanine 


P1400 D-Tryptophan 


P1420 D-Valine 


P1520 Beta-Alanine 

State : Powder Storage : +2/+8 °C Life : 36 months CAS N°107-95-9 


>> Unit / Size of amino acids on request

Vitamins 


>> Unit / Size of vitamins on request 


Vitamins 

P3010 L-Ascorbic Acid (Vitamin C) 


P3020 D-Biotin (Vitamin H) 


P3030 Calcium D-Pantothenate 


P3040 Choline Chloride 


P3050 Folic Acid 


P3060 Myo-Inositol 


P3070 Nicotinamide 


P3080 Nicotinic Acid 


P3090 Pyridoxal Hydrochloride 

State : Crystalline Storage : +2/+8 °C Life : 48 months CAS N° 65-22-5 

P3100 Pyridoxine Hydrochloride 


P3110 Riboflavin 


P3120 Thiamine Hydrochloride 


P3130 Vitamin B12 

State : Crystalline Storage : +2/+8 °C Life : 12 months CAS N° 68-19-9 

P3150 Vitamin E Acetate 



85



86 


O.C.* 

Amino Acids 

I.S.** 

Vitamins 

Composition 

Trypsin L0909 L0910 L0930 L0931 L0940 P0940 X0915 X0920 X0930 X0960 

Liquid Liquid Liquid Liquid Liquid Powder 10x 10x 10x 10x 

mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l 

EDTA / / / / 200 2000 / / / / 

EDTA Disodium Salt Dihydrate / / 221,4 380 / / / / 2214 / 

Potassium Chloride 200 200 400 400 200 / 200 400 4000 4000 

Potassium Phosphate Monobasic Anhydrous 200 200 / 60 200 / 200 60 / / 

Sodium Bicarbonate 580 350 / / / 350 / 3500 

Sodium Chloride 8000 8000 8000 8000 8000 8500 8000 8000 8000 80000 

Sodium Phosphate Dibasic Anhydrous 1150 1150 / 48 1150 / 1150 47,88 / / 

D-Glucose 1000 1000 1000 1000 1000 / 1000 1000 10000 10000 

Phenol Red Sodium Salt 2 / 2 10 / / / / / 50 

Trypsin 1:250 2500 2500 500 2500 500 500 25000 25000 5000 25000 

MEM 50x / 100x concentrate X0552 X0554 X0555 X0556 X0557 

BME 100 x concentrate Liquid Liquid Liquid Liquid Liquid 


Glycine / / / / 750 

L-Alanine / / / / 890 

L-Arginine Monohydrochloride 2100 6320 / / / 

L-Asparagine Monohydrate / / / / 1500 

L-Aspartic acid / / / / 1330 

L-Cystine 1200 1200 / / 

L-Glutamic Acid / / / / 1470 

L-Histidine Monohydrochloride Monohydrate / 2100 / / / 

L-Histidine 800 / / / 

L-Isoleucine 2600 2625 / / / 

L-Leucine 2600 2625 / / / 

L-Lysine Monohydrochloride 3647 3625 / / / 

L-Methionine 750 755 / / / 

L-Phenylalanine 1650 1650 / / / 

L-Proline / / / / 1150 

L-Serine / / / / 1050 

L-Threonine 2400 2380 / / / 

L-Tryptophan 400 510 / / / 

L-Tyrosine 1800 1800 / / / 

L-Valine 2350 2340 / / / 

Sodium Chloride / / 8500 8500 / 

Choline Chloride / / 100 100 / 

D-Biotin / / 100 / / 

D-Ca Pantothenate / / 100 100 / 

Folic Acid / / 100 100 / 

Myo-Inositol / / 200 200 / 

Nicotinamide (Nicotinic acid amide) / / 100 100 / 

Pyridoxal Hydrochloride / / 100 100 / 

Riboflavin / / 10 10 / 

Thiamine Hydrochloride / / 100 100 / 



** Inorganic Salts

I.S.** 

O.C.* 


O.C.* 


** Inorganic Salts 

Lymphosep L0560 

Liquid 

mg/l 

Diatrizoic Acid Dihydrate 95281,959 

EDTA Dihydrate 231 

Sodium Hydroxyde pellets 5860 

Polysaccharose 400 57000 

Colcemid L0040 

Liquid 

mg/l 

Calcium Chloride Dihydrate 133 

Magnesium Chloride Hexahydrate 100 


Potassium Phosphate Monobasic Anhydre 200 



Demecolcine 10 


Composition 


87

Standard Terms of Sales 

88 


The standard terms of sale and delivery shall be deemed to have been accepted for any order placed with 

our company. In the event of dispute, our terms cancel any conflicting clauses and terms printed on the 

orders or correspondence from buyers. Amendments of the initial agreement or any secondary agreement 

shall be valid only if they have been entered into in writing. 

1> Products 

The specifications and figures mentioned in our catalogues are given for information and without commitment. 

Biowest reserves the right to change its products without notice, depending on improvements imposed 

by the technical development. 

2> Orders 

Orders may be sent by letter, fax or e-mail. 

Orders shall be final only when Biowest has confirmed them in writing. 

They must include : 

- The numbers of our catalogues or our offers. If the description is vague, if we ourselves have to make a 

choice, we disclaim liability on this account. The buyer shall bear any costs of return for the resulting noncompliance. 

- Delivery and invoicing addresses 

- Inter-community VAT number 

3> Deliveries 

3.1> Delivery time 

Delivery time shall be confirmed upon receipt of your order. If the products are not in stock, a delivery time 

shall be proposed for information, subject to accidental cases and force majeure. No penalty for late performance 

or damage may be claimed in the event said deliver times are not respected. 

Biowest shall choose the method of dispatch that it considers to be the most suitable for its customer, if 

the latter has not expressed special requirements. 

3.2> Accidental cases and force majeure 

Biowest shall be released from its obligation to deliver in the event of any accidental case or force majeure 

event that impedes either the manufacturing, dispatch or import into France. A force majeure event means 

any event beyond our control, which results in delaying or preventing the performance that could not be reasonably 

controlled or avoided. 

4> Price and invoicing 

The prices on catalogues, printed leaflets, price lists or on-line are given for information. Biowest reserves 

the right to amend same, without notice. Our Prices are guaranteed for the term of validity of the offer or 

the estimate, apart from the change in the price of raw materials, exchange rates of foreign currencies or 

customs duties. The prices invoiced shall always be those in force on the date of the actual delivery. Unless 

otherwise specified in writing, our prices are quoted net and excluding tax. 

Transport costs are defined for France with carriage paid of EUR 250, excluding tax; otherwise transport 

costs are EUR 25, excluding tax, to which a fixed amount of EUR 15, excluding tax, shall be added for parcels 

sent with carbo-ice. For overseas, costs are defined in relation to volumes and end destinations. 

5> Payment 

5.1> Terms and conditions 

Our invoices are payable by cheque, bank or postal transfer, revocable letter of credit, within 30 days, date 

of invoicing net and without discount, unless otherwise agreed in writing. 

Biowest reserves the right to claim an advance payment or a part payment prior to the fulfilment of the 

order. 

5.2> Penalty clause and event of default clause 

By express agreement and except with Biowest’s prior agreement, the non payment of an invoice at due date 

shall give rise, by operation of law, regardless of the method of payment: 

- to a minimum interest of 1.5 times the legal interest rate set by decree on 1 January of each year, 

- the immediate payability of all outstanding amounts. 

Costs, outlay and expenses incurred by Biowest to obtain the payment of goods shall be borne by the customer 

under Article 700 of the New French Code of Civil Procedure (French acronym N.C.P.C.) 

Moreover, pending regularisation, Biowest reserves the right to suspend any subsequent deliveries. 

www.biowest.net


6> Warranty of services and reservations 

6.1> Claims 

The customer must check upon receipt of the products that the delivery is indeed compliant with its order. 

Any claim relating to transport should be made to our services within 48 hours and mentioned on the carrier’s 

receipt. For any other non-apparent defect, we should be notified thereof within a maximum period of 

3 months after receipt of the products and product storage instructions should be respected pending our 

instructions. 

6.2> Return 

No return shall be accepted without the prior and written agreement of our sales department, which shall 

specify the terms and conditions of return. The returned products shall be credited, less a fixed amount for 

the costs of control and return to stock of 15% of the sale price, with a minimum of EUR 35 and only if 

they are in their original condition. 

6.3> Scope of the warranty 

The user must decide that said product is suitable for its specific application. The products of our catalogue 

are devised for laboratory use only (use in-vitro exclusively). They may not be used as medicine, ancillary 

products, as pharmaceutical or cosmetic preparations, farm product and human or veterinary use products. 

The buyer shall be solely responsible for their use. 

6.4> Transfer of risks 

Our goods and their packaging shall always travel at the buyer’s risks, even if they are dispatched carriage 

paid. We disclaim any liability for alterations occurring during transport. In the event of any damage, the 

consignee shall be responsible for notifying the carrier who made delivery of the losses and damage observed 

upon arrival, by registered letter within three days following receipt of the goods, in compliance with 

the regulations of Article 105 of the French Commercial Code and in general for bringing any claim against 

the carrier. 

7> Retention of title clause 

The seller reserves title to the goods until the price thereof in principal and interest has been paid in full. 

Failing payment of the price at the agreed due date, the seller may take back the goods. The sale shall be 

cancelled by operation of law if the seller sees fit and it shall be entitled to part payments already paid in 

consideration of any use of the goods by the buyer. 

8> Disputes 

Courts in the jurisdiction of the place of the company’s registered office shall have sole jurisdiction in the 

event of a dispute of any kind or a dispute relating to the formation or fulfilment of the order. French law 

only shall govern orders placed with Biowest. 


89

Albumin Bovine Serum Fraction V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P6152 . . . . .18 

Albumin Bovine Serum Standard Quality Lyophilised . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P6150 . . . . .18 

Alsever's Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0636 . . . . . .68 

Amphotericin B . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0009 . . . . . .74 

Amphotericin B . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P4030 . . . . .75 

Antibiotic-Antimycotic 100X . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0010 . . . . . .74 

BME 10X w/ Earle's Salts w/o L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0042 . . . . .22 

BME 10X w/ Hanks' Salts w/o L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0026 . . . . .22 

BME Amino Acids 100X w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0552 . . . . .79 

BME Vitamins 100X w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0555 . . . . .79 

BME w/ Earle's Salts w/ L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0043 . . . . . .22 

BME w/ Earle's Salts w/ L-Glutamine w/ 25 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0045 . . . . . .22 

BME w/ Earle's Salts w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0042 . . . . . .22 

BME w/ Earle's Salts w/o L-Glutamine w/ 25 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0044 . . . . . .22 

BME w/ Hanks' Salts w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0046 . . . . . .22 

BME w/ Earle's Salts w/ L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0030 . . . . .22 

BME w/ Hanks' Salts w/ L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0031 . . . . .22 

Bovine Plasma w/ Sodium Citrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S0260 . . . . .16 

Bovine Serum (France Origin) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S0250 . . . . .15 

Bull Calf Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S1400 . . . . .15 

Calf Plasma w/ Sodium Citrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S0410 . . . . .16 

Calf Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S0400 . . . . .15 

Cat (Feline) serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2800 . . . . .16 

Cell Culture Water Pyrogen free . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0970 . . . . . .81 

Chicken Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S0500 . . . . .16 

Ciprofloxacin Hydrochloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0016 . . . . .75 

CMRL 1066 w/ L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0058 . . . . .23 

CMRL 1066 w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0053 . . . . . .23 

Colcemid 10 µg / ml in PBS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0040 . . . . . .81 

DMEM F12 w/ L-Glutamine w/ 15 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0093 . . . . . .25 

DMEM F12 w/ L-Glutamine w/ 25 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0095 . . . . . .25 

DMEM F12 w/ L-Glutamine w/o Sodium Bicarbonate w/ 15 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0095 . . . . .25 

DMEM F12 w/ stable Glutamine w/ 15 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0092 . . . . . .25 

DMEM F12 w/o L-Glutamine w/ 15 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0094 . . . . . .25 

DMEM F12 w/o L-Glutamine w/ 25 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0096 . . . . . .25 

DMEM F12 w/o L-Glutamine w/o Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0090 . . . . . .25 

DMEM F12 w/o L-Glutamine w/o Hepes w/o Glucose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0091 . . . . . .25 

DMEM High Glucose w/ L-Glutamine w/ Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0104 . . . . . .23 

DMEM High Glucose w/ L-Glutamine w/o Sodium Bicarbonate w/ Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0102 . . . . .24 

DMEM High Glucose w/ L-Glutamine w/o Sodium Bicarbonate w/o Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0103 . . . . .24 

DMEM High Glucose w/ L-Glutamine w/o Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0102 . . . . . .23 

DMEM High Glucose w/ stable Glutamine w/ 25mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0107 . . . . . .23 

DMEM High Glucose w/ stable Glutamine w/ Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0103 . . . . . .23 

DMEM High Glucose w/o L-Glutamine w/ 25mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0100 . . . . . .23 

DMEM High Glucose w/o L-Glutamine w/ Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0106 . . . . . .23 

DMEM High Glucose w/o L-Glutamine w/o Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0101 . . . . . .23 

DMEM Low Glucose w/ L-Glutamine w/ Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0060 . . . . . .24 

DMEM Low Glucose w/ L-Glutamine w/ Sodium Pyruvate w/ 25mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0065 . . . . . .24 

DMEM Low Glucose w/ L-Glutamine w/o Sodium Bicarbonate w/ Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0061 . . . . .24 

DMEM Low Glucose w/ L-Glutamine w/o Sodium Bicarbonate w/ Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0070 . . . . . .24 

DMEM Low Glucose w/ stable Glutamine w/ Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0066 . . . . . .24 

DMEM Low Glucose w/o L-Glutamine w/ Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0064 . . . . . .24 

DMEM Low Glucose w/o L-Glutamine w/ Sodium Pyruvate w/ 25 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0067 . . . . . .24 

Dog (Canine) Beagle Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2901 . . . . .16 

Dog (Canine) Plasma w/ Sodium Citrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2910 . . . . .17 

Dog (Canine)serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2900 . . . . .16 

Donkey Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2170 . . . . .15 

Donor Bovine Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S0600 . . . . .15 

Donor Calf Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S0450 . . . . .15 

Donor Foal Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S0800 . . . . .15 

Donor Horse Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S0900 . . . . .15 

Donor Horse Serum (from pregnant Horses) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S0950 . . . . .15 

Dulbecco's Phosphate Buffered Saline 10X w/ Calcium w/ Magnesium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0520 . . . . .65 

Dulbecco's Phosphate Buffered Saline 10X w/o Calcium w/o Magnesium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0515 . . . . .65 

Dulbecco's Phosphate Buffered Saline w/ Calcium w/ Magnesium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0625 . . . . . .65 

Dulbecco's Phosphate Buffered Saline w/o Calcium w/o Magnesium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0750 . . . . .65 

Dulbecco's Phosphate Buffered Saline w/o Calcium w/o Magnesium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0615 . . . . . .65 

Earle's Balanced Salt Solution 10X w/ Calcium w/ Magnesium w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . .X0112 . . . . .66 

Earle's Balanced Salt Solution 10x w/o Calcium w/o Magnesium w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . .X0113 . . . . .66 

Earle's Balanced Salt Solution w/ Calcium w/ Magnesium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0602 . . . . . .66 

Earle's Balanced Salt Solution w/o Calcium w/o Magnesium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0601 . . . . . .66 

Earle's Balanced Salt Solution w/o Calcium w/o Magnesium w/o Phenol Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0600 . . . . . .66 

Fetal Bovine Serum Australia Origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S1700 . . . . .14 

Fetal Bovine Serum BSE tested France Origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S1820 . . . . .14 

Fetal Bovine Serum Canada Origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S1500 . . . . .14 

Fetal Bovine Serum Central America Origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S1600 . . . . .14 

Fetal Bovine Serum Chile Origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S1560 . . . . .14 

Fetal Bovine Serum Denmark Origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S1750 . . . . .14 

Fetal Bovine Serum Mexico Origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S1650 . . . . .14 

90 

Glossary 

Glossary 


A 

B 

C 

D 

E 

F 

Product Cat. N° Page

Fetal Bovine Serum South America Origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S1810 . . . . .14 

Fetal Bovine Serum USA Origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S1520 . . . . .14 

Fetal Horse Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S0960 . . . . .15 

G-418 Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0015 . . . . . .74 

G-418 Sulfate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0017 . . . . .75 

Gentamicin Sulfate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P4020 . . . . .75 

Gentamicin Sulfate 10mg/ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0011 . . . . . .74 

Gentamicin Sulfate 50mg/ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0012 . . . . . .74 

Glasgow MEM BHK 21 10X w/ L-Glutamine w/o Tryptose Phosphate Broth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0122 . . . . .26 

Glasgow MEM BHK 21 10X w/o L-Glutamine w/o Tryptose Phosphate Broth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0123 . . . . .26 

Glasgow MEM BHK 21 w/ L-Glutamine w/o Sodium Bicarbonate w/o Tryptose Phosphate Broth . . . . . . . . . . . . . . . . . . . . .P0120 . . . . .26 

Glasgow MEM BHK 21 w/ L-Glutamine w/o Tryptose Phosphate Broth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0221 . . . . . .25 

Glasgow MEM BHK 21 w/o L-Glutamine w/o Tryptose Phosphate Broth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0222 . . . . . .25 

Glutamine stable 100X, 200 mM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0551 . . . . .80 

Glutamine-Gentamicin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0013 . . . . . .74 

Glutamine-Penicillin-Streptomycin 100X . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0014 . . . . . .74 

Goat Plasma w/ Sodium Citrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2010 . . . . .16 

Goat Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2000 . . . . .15 

Guinea Pig Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2450 . . . . .16 

Ham's F10 10X w/ L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0150 . . . . .26 

Ham's F10 w/ L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0140 . . . . . .26 

Ham's F10 w/ L-Glutamine w/ 25 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0130 . . . . . .26 

Ham's F10 w/ L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0146 . . . . .26 

Ham's F10 w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0145 . . . . . .26 

Ham's F12 10X w/o L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0155 . . . . .27 

Ham's F12 w/ L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0135 . . . . . .27 

Ham's F12 w/ L-Glutamine w/ 25 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0132 . . . . . .27 

Ham's F12 w/ L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0134 . . . . .27 

Ham's F12 w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0136 . . . . . .27 

Ham's F12 w/o L-Glutamine w/ 25 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0133 . . . . . .27 

Ham's F14 w/ 6 g/L Glucose w/ 1mg/L ATP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0138 . . . . . .28 

Hanks' Balanced Salt Solution w/o Calcium w/o Magnesium w/ Sodium Bicarbonate w/o Phenol Red . . . . . . . . . . . . . . . . .L0607 . . . . . .66 

Hanks' Balanced Salt Solution 10X w/ Calcium w/ Magnesium w/o Sodium Bicarbonate w/ Phenol Red . . . . . . . . . . . . . . .X0509 . . . . .67 

Hanks' Balanced Salt Solution 10X w/o Calcium w/o Magnesium w/ Sodium Bicarbonate w/o Phenol Red . . . . . . . . . . . . .X0510 . . . . .67 

Hanks' Balanced Salt Solution 10X w/o Calcium w/o Magnesium w/o Sodium Bicarbonate w/ Phenol Red . . . . . . . . . . . . .X0513 . . . . .67 

Hanks' Balanced Salt Solution 10X w/o Calcium w/o Magnesium w/o Sodium Bicarbonate w/o Phenol Red . . . . . . . . . . . .X0507 . . . . .67 

Hanks' Balanced Salt Solution w/ Calcium w/ Magnesium w/ Sodium Bicarbonate w/ Phenol Red . . . . . . . . . . . . . . . . . . . .L0606 . . . . . .66 

Hanks' Balanced Salt Solution w/ Calcium w/ Magnesium w/ Sodium Bicarbonate w/o Phenol Red . . . . . . . . . . . . . . . . . . .L0612 . . . . . .67 

Hanks' Balanced Salt Solution w/ Calcium w/ Magnesium w/o Sodium Bicarbonate w/ Phenol Red . . . . . . . . . . . . . . . . . . .L0608 . . . . . .66 

Hanks' Balanced Salt Solution w/o Calcium w/o Magnesium w/ Sodium Bicarbonate w/ Phenol Red . . . . . . . . . . . . . . . . . .L0611 . . . . . .67 

Hanks' Balanced Salt Solution w/o Calcium w/o Magnesium w/o Sodium Bicarbonate w/ Phenol Red . . . . . . . . . . . . . . . . .L0609 . . . . . .66 

Hanks' Balanced Salt Solution w/o Calcium w/o Magnesium w/o Sodium Bicarbonate w/o Phenol Red . . . . . . . . . . . . . . . .L0605 . . . . . .66 

Hanks' Balanced Salts w/ Calcium w/ Magnesium w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0154 . . . . .67 

Hanks' Balanced Salts w/o Calcium w/o Magnesium w/o Sodium Bicarbonate w/o Phenol Red . . . . . . . . . . . . . . . . . . . . . . .P0153 . . . . .67 

Hepes Buffer 1 M . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0180 . . . . . .68 

Hepes, cell culture tested . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P5455 . . . . .68 

Horse Plasma w/ Sodium Citrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S0920 . . . . .16 

Horse Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S0910 . . . . .15 

Hygromycin B . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0019 . . . . .75 

IMDM w/ L- Glutamine w/ 25 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0190 . . . . . .28 

IMDM w/ L- Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0191 . . . . .28 

IMDM w/ stable Glutamine w/ 25 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0191 . . . . . .28 

IMDM w/o L- Glutamine w/o Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0192 . . . . . .28 

Lamb Plasma w/ Sodium Citrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2310 . . . . .16 

Lamb Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2300 . . . . .15 

Leibovitz L-15 Medium w/ L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0350 . . . . .29 

Leibovitz L-15 Medium w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0300 . . . . . .29 

L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P1012 . . . . .80 

L-Glutamine 100X, 200mM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0550 . . . . .80 

Lymphosep, Lymphozyte Separation Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0560 . . . . . .79 

McCoy's 5A w/ L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0210 . . . . . .29 

McCoy's 5A w/ L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0390 . . . . .29 

McCoy's 5A w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0211 . . . . . .29 

MCDB 105 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L1200 . . . . . .35 

MCDB 131 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L1202 . . . . . .35 

MCDB 151 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L1203 . . . . . .35 

MCDB 201 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L1205 . . . . . .35 

MCDB 302 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L1206 . . . . . .35 

Medium 199 w/ Hanks' Salts w/ L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0330 . . . . . .32 

Medium 199 w/ Earle's Mod. Salts w/ L-Glutamine w/ 1,25 g/L Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0355 . . . . . .32 

Medium 199 w/ Earle's Salts w/ L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0420 . . . . .32 

Medium 199 w/ Earles' Salts w/ L-Glutamine w/o Sodium Bicarbonate w/ 25mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . .P0425 . . . . .32 

Medium 199 w/ Earle's Salts w/ L-Glutamine w/ 25mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0360 . . . . . .32 

Medium 199 w/ Earles' Salts w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0356 . . . . . .32 

Medium 199 w/ Hanks' Salts w/ L-Glutamine w/ 25mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0331 . . . . . .32 

Medium 199 w/ Hanks' Salts w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0325 . . . . . .32 

Medium 199 w/ Hanks' Salts w/o L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0410 . . . . .32 

MEM 10X w/ Earle's Salts w/o L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0311 . . . . .31 

MEM 10X w/ Hank's Salts w/o L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0321 . . . . .31 

91 


Glossary 

F 

G 

H 

I 

L 

M 


MEM Alpha Modification w/ Earle's Salts w/ L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0440 . . . . .32 

MEM Alpha w/ L-Glutamine w/o Ribunocleosides w/o Deoxyribonucleosides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0475 . . . . . .31 

MEM Alpha w/o L-Glutamine w/o Ribunocleosides w/o Deoxyribonucleosides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0476 . . . . . .31 

MEM Amino Acids 50X w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0554 . . . . .79 

MEM Autoclave w/ Earle's Salts w/o L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0460 . . . . .31 

MEM Non Essential Amino Acids 100X w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0557 . . . . .79 

MEM Vitamins 100X w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0556 . . . . .79 

MEM w/ Earle's Salts w/ L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0415 . . . . . .30 

MEM w/ Earle's Salts w/ L-Glutamine w/o Sodium Bicarbonate w/ 25mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0452 . . . . .30 

MEM w/ Earle's Salts w/ L-Glutamine w/ 25mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0444 . . . . . .30 

MEM w/ Earle's Salts w/ L-Glutamine w/ NEAA w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0450 . . . . .30 

MEM w/ Earle's Salts w/ L-Glutamine w/o NEAA w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0451 . . . . .30 

MEM w/ Earle's Salts w/ stable Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0416 . . . . . .30 

MEM w/ Earle's Salts w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0440 . . . . . .30 

MEM w/ Earle's Salts w/o L-Glutamine w/ 25mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0445 . . . . . .30 

MEM w/ Earle's Salts w/o L-Glutamine w/NEAA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0430 . . . . . .30 

MEM w/ Hanks' Salts w/ L-Glutamine w/ NEAA w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0515 . . . . .31 

MEM w/ Hanks' Salts w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0465 . . . . . .31 

MEM w/ Hanks' Salts w/o L-Glutamine w/ 25mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0470 . . . . . .31 

Mouse Plasma w/ Lithium Heparin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2162 . . . . .17 

Mouse Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2160 . . . . .16 

N-Acetyl-L-Alanyl-L-Glutamine, stable Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P1029 . . . . .80 

NaCl Salt Solution 0.85 % . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0640 . . . . . .68 

NaCl Salt solution 0.9% (non sterile) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0641 . . . . . .68 

Nanomycopulitine Concentrat 20 x . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L-X16 . . . . . .74 

New Born Calf Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S0750 . . . . .15 

Penicillin G Sodium Salt,~1.650 units per mg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0018 . . . . .75 

Penicillin-streptomycin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0018 . . . . . .74 

Penicillin-streptomycin Solution 100X . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0022 . . . . . .74 

Phenol Red Sodium Salt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P5648 . . . . .81 

Phenol Red Solution 0.5 % . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0541 . . . . . .81 

Pig Plasma w/ Sodium Citrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2410 . . . . .17 

Pig Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2400 . . . . .16 

Polymyxin B Sulfate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0017 . . . . . .74 

Potassium Chloride 0.075 M . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0643 . . . . . .68 

Rabbit Plasma w/ EDTA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2600 . . . . .16 

Rabbit Serum (France Origin) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2500 . . . . .15 

Rat Plasma w/ Lithium Heparin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2140 . . . . .17 

Rat Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2150 . . . . .16 

RPMI 1640 Dutch Modification w/o L-Glutamine w/ 1g/l Sodium Bicarbonate w/ 20mM Hepes . . . . . . . . . . . . . . . . . . . . . .L0492 . . . . . .33 

RPMI 1640 w/ L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0500 . . . . . .33 

RPMI 1640 w/ L-Glutamine w/ 25mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0495 . . . . . .33 

RPMI 1640 w/ L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0504 . . . . . .33 

RPMI 1640 w/ L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0860 . . . . .34 

RPMI 1640 w/ L-Glutamine w/o Sodium Bicarbonate w/25 mM Hepes w/o Phenol Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0876 . . . . .34 

RPMI 1640 w/ L-Glutamine w/o Sodium Bicarbonate w/o Glucose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0883 . . . . .34 

RPMI 1640 w/ L-Glutamine w/o Sodium Bicarbonate w/o Phenol Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0880 . . . . .34 

RPMI 1640 w/ stable Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0498 . . . . . .33 

RPMI 1640 w/ stable Glutamine w/ 25mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0496 . . . . . .33 

RPMI 1640 w/ stable Glutamine w/25 mM Hepes w/o Phenol Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0877 . . . . .34 

RPMI 1640 w/o L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0501 . . . . . .33 

RPMI 1640 w/o L-Glutamine w/ 25 mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0490 . . . . . .33 

RPMI 1640 w/o L-Glutamine w/o Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0503 . . . . . .33 

RPMI 1640 w/o L-Glutamine w/o Phenol Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0505 . . . . . .33 

RPMI 1640 w/o L-Glutamine w/o Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0870 . . . . .34 

RPMI 1640 w/o L-Glutamine w/o Sodium Bicarbonate w/ 25mM Hepes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0875 . . . . .34 

RPMI 1640 w/o L-Glutamine w/o Sodium Bicarbonate w/o Phenol Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0871 . . . . .34 

Schneider's Drosophila Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0207 . . . . . .35 

Sheep Plasma w/ Sodium Citrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2360 . . . . .16 

Sheep Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .S2350 . . . . .15 

Sodium Bicarbonate 7.5 % . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0680 . . . . . .68 

Sodium Pyruvate 100 mM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0642 . . . . . .68 

Streptomycin Sulfate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0025 . . . . .75 

Trypan blue (Direct blue 14) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0935 . . . . .82 

Trypan blue 0.5 % Solution (non sterile) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0990 . . . . . .82 

Trypsin - EDTA 1X Lyophilised w/ Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P0940 . . . . .78 

Trypsin 0.25 % in PBS w/o Calcium w/o Magnesium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0910 . . . . . .78 

Trypsin 0.25 % in PBS w/o Calcium w/o Magnesium w/ Phenol red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0909 . . . . . .78 

Trypsin 0.25% - EDTA in PBS w/o Calcium w/o Magnesium w/ Phenol Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0931 . . . . . .78 

Trypsin 1:250 powder (porcine) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P5957 . . . . .78 

Trypsin 2.5 % in HBSS w/o Calcium w/o Magnesium w/o Phenol Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0920 . . . . .78 

Trypsin 2.5 % in PBS w/o Calcium w/o Magnesium w/o Phenol Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0915 . . . . .78 

Trypsin 2.5% 10X in Puck`s Salts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0960 . . . . .78 

Trypsin-EDTA 10X . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .X0930 . . . . .78 

Trypsin-EDTA 1X in PBS w/o Calcium w/o Magnesium w/ Phenol Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0930 . . . . . .78 

Trypsin-EDTA 1X in PBS w/o Calcium w/o Magnesium w/o Phenol Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0940 . . . . . .78 

Versen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .L0630 . . . . . .68 

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Biowest S.A.S. 

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Cell Culture Medium

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