Clinical and Technical Review - Tecomedical

8
Bone marker and characteristics
Biomarker Method Main
Sample
Type
Formation
BAP
Osteocalcin
Intact
Osteocalcin
N-mid
PINP
PICP = CICP
Resorption
ICTP =
CTX-MMP
Others
ELISA
IRMA/
ELISA
ELISA
RIA
ELISA
Serum
Serum
Serum
Serum
Serum
A(a) B C D E F G H
Pre- &
postmenopause
+
+
+
+
NA
Analytical
variation
+
+
+
+
+
Treatment
response
Short
time
change
Within
person
variation
Daily
variation
Food
intake
TRAP 5b ELISA Serum ± + ± ± NA + + ±
DPD ELISA Urine + + + + + - + +
NTx ELISA
CTx ELISA
+
+
+
+
+
Sample
stability
Scores sind: + (yes), - (no), ± (fair/indeterminate), NA (not available)
(a) Letters correspond to following, the marker:
H. preferably demonstrates high stability in the biological
A. shows a difference in the rate of bone turnover pre-and
specimen
post-menopause
(b) In contrast to OPG, sRANKL showed no response to
B. demonstrates minimal analytical variation
bisphosphonate treatment of osteoporotic postmeno-
C. significantly changes in response to treatment
pausal women. However, PTH treatment of glucocorticoid
D. detect changes in short time interval (months)
induced osteoporotic women resulted in a fast (1 month)
E. demonstrates minimal within person (biological) variation and sustained increase of sRANKL levels.
F. preferably demonstrates little variation over the day
(c) Cathepsin K is elevated in patients with established
G. no influence by food intake
rheumatoid arthritis.
+
+
+
+
+
+
±
±
±
+
+
+
+
+
±
+
+
+
+
+
+
-
+
+
+
5 days
2–8 °C
4hours
2–8 °C
5 days
2–8 °C
5 days
2–8 °C
5 days
2–8 °C
2 days
2–8 °C
7 days
2–8 °C
Serum + + + + + - ± ± 1day
2–8 °C
Urine + + + + ± - ± +
3 days
2–8 °C
Serum + + + + + - - + 1day
2–8 °C
Urine + + + + ± - - +
ELISA Serum - + - - NA ± + +
7 days
2–8 °C
5 days
2–8 °C
sRANKL ELISA Plasma NA + ± (b) - NA NA + ± 1day
2–8 °C
Osteoprotegerin
(OPG)
ELISA Plasma NA + + - NA NA + ± 1day
2–8 °C
Cathepsine K ELISA Serum NA (c) + NA NA NA NA + ±
2 days
2–8 °C