GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS
GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS
GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS
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loading of the test substance relative to the weight of sediment in each test vessel. This result<br />
indicates that the test substance exhibits some toxicity to this species of sediment dwelling<br />
organism. Furthermore, it should be noted that the levels of chemical in the piles of drill<br />
cuttings may be up to 100 000 mg/kg which is well in excess of 1 250 mg/kg. Consequently,<br />
the piles of drill cuttings may be toxic to this species.<br />
Alga Growth Inhibition Test (Environment & Resource Technology Ltd 1995)<br />
The tests on the effect of <strong>C16</strong>-<strong>18</strong> alpha olefins, isomerised to the marine alga Skeletonema<br />
costatum was conducted in accordance with (US EPA 1985) using five WAFs of the test<br />
substance prepared with nominal loadings of test substance of 560, 1 000, 1 800, 3 200 and 5<br />
600 mg/L in nutrient enriched sea water, and a control. The tests were conducted in duplicate<br />
over a 72 hour period at a temperature of 20°C and pH between 8.39 and 9.01. After 72<br />
hours, a 4% inhibition in biomass was observed for the media containing 3 200 mg/L WAF,<br />
while 12% inhibition was observed for the media containing the highest WAF loading.<br />
Accordingly the NOEL is 1 800 mg/L, the 72 hour EbL50 is greater than 5 600 mg/L, and the<br />
test substance may be considered to be practically non toxic to this species up to the limit of<br />
its water solubility.<br />
The table above also contains entries for other tests on Acartia tonsa (marine copepod), and<br />
Skeletenoma costatum (marine algae) performed on material known as “Olefin Isomers IV”.<br />
These tests were performed by the Norwegian Institute for Water Research, and summary<br />
reports only were provided. While the test samples were isomerised olefins intended for use<br />
in drilling muds, there is no information on the relevant molecular weight range. However,<br />
the results listed are in general accord with those of the better detailed reports, and are<br />
included in the table for completion. Similarly, a test report on the toxicity of a Syn-Teq<br />
drilling mud containing isomerised olefins to the sediment reworker Corophium volutator<br />
was also submitted with the application (Orkney Water Test Centre 1994). Again the exact<br />
nature of the olefin components of the mud were not specified, but the results are in general<br />
agreement with those of the other test on this species, and need not be discussed further.<br />
Indigenous Species<br />
Toxicity testing on three representative species indigenous to the Western Australian marine<br />
environment has also been performed, and fairly detailed summary reports also included in<br />
the application. The results of these tests are given in the table below.<br />
Toxicity Tests on Western Australian Marine Species (Tsvetnenko YB Evans LH Gorrie J )<br />
Test Species Results (WAF - Nominal)<br />
Acute Toxicity to Marine<br />
Invertebrate<br />
Acute Toxicity to Marine<br />
Copepod<br />
Penaeus monodon<br />
(Tiger prawn)<br />
Gladioferens imparipes<br />
(copepod)<br />
LL50 (96 h)>21.7 g/L<br />
NOEL (96 h) = 11.2 g/L<br />
LL50 (48 h) >224.2 g/L<br />
NOEL (48 h) 242.4 g/L<br />
FULL PUBLIC REPORT 26 April 2000<br />
NA/713 Page 84 of 100