GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS

10.1.C C20-24 Alpha Olefins, Branched and Linear,
• Fish Acute Toxicity Test (Safepharm Laboratories Limited 1998)
• Alga Growth Inhibition Test (Safepharm Laboratories Limited 1998)
• Acute Immobilisation Test (Safepharm Laboratories Limited 1998)
The definitive tests on all three species were performed using media made up with WAFs
prepared at nominal loading of 1 000 mg/L of the test substance. The methodologies
employed were in accordance with OECD TG 301, 302 and 303 essentially as described
above. The daphnia test was conducted with four replicates (5 animals per test vessel) and
the alga test was conducted with six replicates. In all cases the NOEL was greater than the
1 000 mg/L WAF loading, indicating this fraction as non toxic up to the limit of its water
solubility.
Activated Sludge Respiration Inhibition Test (Safepharm Laboratories Limited 1998)
Using OECD TG 209, the study found that the test substance has no effect on aerobic
bacterial metabolism after 3 hours exposure to media containing WAF prepared at 1 000
mg/L loading. Although the use pattern of Gulftene 16-18 is such that entry into the sewer
system is unlikely, the results of this study are included for completeness. Similarly, in the
various tests for aerobic biodegradation, the reports indicated no observable inhibition of
bacterial respiration.
FULL PUBLIC REPORT 26 April 2000
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