GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS

with 42% less growth (relative to the controls) recorded over 72 hours, and 30% less over the
96 hour observation period.
The results of this study indicate that the EbL50 is greater than 1 000 mg/L (nominal WAF),
but that the NOEL is less than 1 000 mg/L. The results indicate that 1-tetradecene is non
toxic to this species up to the limits of its water solubility.
10.1.b 1-hexadecene
The tests were performed using essentially the same methodology as for the tests on
1-tetradecene. Again WAFs with nominal loadings up to 1 000 mg/L of test substance were
used as media in the definitive tests.
Fish Acute Toxicity Test (Huntingdon Research Centre 1993)
Using OECD TG 303, the NOEL for rainbow trout was greater than 1 000 mg/L WAF
loading.
Acute Immobilisation Test (Huntingdon Research Centre 1993)
The data for daphnia, conducted in media containing 1, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100%
of the 1 000 mg/L WAF, indicate some immobilisation, and the 48 hour LL50 was calculated
as 53% of the (nominal) 1 000 mg/L WAF, or nominally 530 mg/L. The corresponding
NOEL was calculated as 18% of the 1 000 mg/L WAF, or nominally at a WAF prepared at
180 mg/L loading.
Alga Growth Inhibition Test (Huntingdon Research Centre 1993)
The test on green alga indicated no inhibitory effects for either growth of biomass, or rate of
biomass production in media containing 100% WAF prepared at a loading of 1 000 mg/L.
Summary
Test results for 1-hexadecene were similar to those for 1-tetradecene and indicate that the 1hexadecene
is non toxic to the fresh water fish and alga against which it was tested.
However, the results for daphnia indicated some immobilisation at nominal WAF loadings
above 180 mg/L, but it difficult to establish the true significance of this result as there is no
data on the actual levels to which the organisms were exposed.
FULL PUBLIC REPORT 26 April 2000
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