GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS
GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS
GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS
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with 42% less growth (relative to the controls) recorded over 72 hours, and 30% less over the<br />
96 hour observation period.<br />
The results of this study indicate that the EbL50 is greater than 1 000 mg/L (nominal WAF),<br />
but that the NOEL is less than 1 000 mg/L. The results indicate that 1-tetradecene is non<br />
toxic to this species up to the limits of its water solubility.<br />
10.1.b 1-hexadecene<br />
The tests were performed using essentially the same methodology as for the tests on<br />
1-tetradecene. Again WAFs with nominal loadings up to 1 000 mg/L of test substance were<br />
used as media in the definitive tests.<br />
Fish Acute Toxicity Test (Huntingdon Research Centre 1993)<br />
Using OECD TG 303, the NOEL for rainbow trout was greater than 1 000 mg/L WAF<br />
loading.<br />
Acute Immobilisation Test (Huntingdon Research Centre 1993)<br />
The data for daphnia, conducted in media containing 1, 1.8, 3.2, 5.6, 10, <strong>18</strong>, 32, 56 and 100%<br />
of the 1 000 mg/L WAF, indicate some immobilisation, and the 48 hour LL50 was calculated<br />
as 53% of the (nominal) 1 000 mg/L WAF, or nominally 530 mg/L. The corresponding<br />
NOEL was calculated as <strong>18</strong>% of the 1 000 mg/L WAF, or nominally at a WAF prepared at<br />
<strong>18</strong>0 mg/L loading.<br />
Alga Growth Inhibition Test (Huntingdon Research Centre 1993)<br />
The test on green alga indicated no inhibitory effects for either growth of biomass, or rate of<br />
biomass production in media containing 100% WAF prepared at a loading of 1 000 mg/L.<br />
Summary<br />
Test results for 1-hexadecene were similar to those for 1-tetradecene and indicate that the 1hexadecene<br />
is non toxic to the fresh water fish and alga against which it was tested.<br />
However, the results for daphnia indicated some immobilisation at nominal WAF loadings<br />
above <strong>18</strong>0 mg/L, but it difficult to establish the true significance of this result as there is no<br />
data on the actual levels to which the organisms were exposed.<br />
FULL PUBLIC REPORT 26 April 2000<br />
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