GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS

10.1.a 1-tetradecene
Fish Acute Toxicity Test (Wildlife International Ltd 1995)
Range finding tests on rainbow trout were performed using WAF of the test substance made
up in filtered well water (no chlorine) at nominal loadings of 0 (control), 10, 100 and 1 000
mg/L in accordance with OECD TG 303. The WAF test media were made up by stirring the
requisite quantity of test substance into the water for around 24 hours, allowing to settle for
approximately one hour, and then siphoning of the aqueous phase containing the WAF. The
WAFs prepared in this manner were clear and colourless, and apparently devoid of
undissolved material or oil droplets. The tests were conducted over a 96 hour period at a
controlled temperature of 15±2°C, and the test media was replaced daily in a batchwise
manner. Five fish were tested at each WAF loading, and no mortalities or behavioural
aberrations were observed over the duration of the tests.
A definitive study was performed in duplicate using a control and the 1 000 mg/L WAF, with
seven fish in each test vessel. The pH was always between 7.7 and 8.4, the temperature was
15±2°C, water hardness around 140 mg/L as CaCO3 and the dissolved oxygen levels between
5.2 and 9.3 mg/L. Again, no deaths or other effects were observed in the test specimens. It is
concluded that the test substance is not toxic to this species of fish, up to the limits of its
water solubility.
Acute Immobilisation Test (Wildlife International Ltd 1995)
The range finding test for acute immobilisation for Daphnia magna was performed over 48
hours using WAFs of the test substance made up in filtered well water at nominal loadings of
0 (control), 10, 100 and 1 000 mg/L, and ten daphnia were tested at each concentration. The
temperature was maintained at 20±1°C, and no irreversible immobilisation or other
behavioural aberrations were observed over the 48 hour test period. A definitive test was
performed using a WAF containing 1 000 mg/L of test substance. Four replicate tests were
run, together with one control, with five daphnia per test vessel. Temperature was maintained
at 20±1°C, pH was always between 8.4±0.1, while dissolved oxygen levels were between 7.9
and 8.1 mg/L. As with the range finding tests, no immobilisation or other effects were
observed, and it is concluded that the test substance is not toxic to the daphnia up to the limits
of its water solubility.
Alga Growth Inhibition Test (Wildlife International Ltd 1995)
Range finding tests on the green alga Selanastrum capricornutum with WAFs prepared at
nominal loadings of up to 1 000 mg/L established that less than 50% inhibition of growth
occurred up to the highest loading. Accordingly, the definitive test was conducted using only
the WAF containing 1 000 mg/L, together with controls. Three replicates of the WAF
containing medium were conducted, while six replicates of the control were used. The tests
was performed over a 96 hour incubation period at 23±0.5°C at pH 7.4-8.7, and both algal
biomass and the rate of biomass growth were measured. For the control group, greater than
16 fold increase in biomass took place over three days as reckoned from the area under the
growth curve. However, for the WAF-containing group the growth was slightly attenuated,
FULL PUBLIC REPORT 26 April 2000
NA/713 Page 78 of 100