GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS

Clinical observations: No mortality;
No clinical signs of toxicity;
Positive control group animals were sacrificed 24 hours
after dosing.
Micronuclei score: No significant increase in micronucleated polychromatic
erythrocytes (PCE) due to treatment with test substance at
either sampling time; the positive control caused a
significant increase in micronucleated PCE.
Test method: OECD TG 474
Result: Gulftene 12-16 did not induce a significant increase in
micronucleated PCEs in bone marrow cells of the mouse
in vivo.
9.3.2.2 Mammalian Erythrocyte Micronucleus Test Using C20-C24 alkenes,
branched and linear (Safepharm Laboratories Limited 1998)
Test substance: C20-C24 alkenes, branched and linear
Species/strain: mouse/Crl:CD-1 (ICR) BR
Number and sex of animals: 7 males/24 hour, vehicle and positive control, and mid,
low and high dose group;
7 males/48 hour, vehicle control and high dose group;
5 males/positive control group.
Doses/Method of
administration:
Test substance: 500 mg/kg (low), 1 000 mg/kg (mid) or
2 000 mg/kg (high);
Positive control: cyclophosphamide 50 mg/kg;
Vehicle control: arachis oil;
All administered via intraperitoneal injection at a constant
volume of 10 mL/kg bw. Positive control was
administered orally.
Sampling schedule: Vehicle and positive control, low, mid and high dose
animals were sacrificed 24 hours after dosing.
Remaining animals of the vehicle control group and high
dose animals were sacrificed 48 hours after dosing.
Clinical observations: No mortality.
No clinical signs of toxicity.
Micronuclei score: No significant increase in micronucleated PCE due to
treatment with test substance at either sampling time. No
statistically significant decrease in the PCE/NCE ratio at
24 or 48 hours.
FULL PUBLIC REPORT 26 April 2000
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