GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS

Result: C20-C24 alkenes, branched and linear were not considered
mutagenic in the bacterial strains tested
9.3.1.2 Genetic Toxicology: DNA Damage and Repair/Unscheduled DNA
Synthesis (UDS) using Gulftene 12-16 In Vitro (Gulf Life Sciences Center
1984)
Test substance: Gulftene 12-16
Cells: Primary hepatocytes from Fischer 344 rats
Dosing schedule: 0, 100, 1 000, 2 000, 4 000 µg/mL of test substance was
tested in triplicate:
Positive Control: 2-acetylaminofluorene 0.2 µg/mL;
Cultures were exposed to test substance and 1 mCi/mL 3 Hthymidine
for 18 hours.
Test method: OECD TG 482
Comment: In the range finding study, cytotoxicity was reported at and
above 256 µg/mL. Toxicity data was not available for the
main test.
The test substance at any concentration did not elicit an
increased mean net nuclear grain count above the concurrent
negative control.
The positive control gave the expected response for UDS.
Result: Gulftene 12-16 was negative for unscheduled DNA
synthesis
9.3.1.3 BALB/3T3 Transformation Test Using Gulftene 12-16 (Gulf Life Sciences
Center 1983)
Test substance: Gulftene 12-16
Cells: Mouse embryo cells BALB/3T3-A31-1-1
Dosing schedule: 0, 10, 20, 30, 1 500 µg/mL of test substance was tested in
duplicate:
Positive Control: 3-methylcholanthrene 1 µg/mL;
Cultures were exposed to test substance for two days.
Test method: Not stated
Comment: Cytotoxicity was evident at 20 µg/mL and above leaving
only one viable dose level, 10 µg/mL.
The number and type of transformed foci at any test
substance concentration was not increased above the
FULL PUBLIC REPORT 26 April 2000
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