GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS

Terminal kill schedule Day 43 – euthanasia of unselected males (4 rats/sex/group).
Days 45 to 47 – neurotoxicity and clinical pathology
evaluations (8 rats/sex/group), histopathology (5
rats/sex/group).
Days 42 to 51 – euthanasia and necropsy of breeding
females (F0)and F1 pups.
Test methods: OECD TG 422 (modified)
Mortality:
F0 males and satellite females:
Nil.
F0 females:
In the 500 mg/kg/day group, one female with evidence of mating failed to deliver and was
euthanised on post breeding day 25 and one female was euthanised with total litter loss on
Day 43.
Clinical observations:
F0 males, satellite females and F0 females:
Urine stain and salivation was noted in the 500 and 1 000 mg/kg/day groups. Other
observations were noted sporadically.
Functional Observation Battery (FOB) and Motor Activity:
F0 males and satellite females:
No test related differences in the FOB and motor activity tests between the control and
treated groups.
Clinical Pathology:
F0 males and satellite females:
Serum Chemistry:
Significantly increased alanine transferase (ALT) activity in males. In females,
significantly decreased sodium values at all treatment doses and significantly increased
cholesterol in the 500 and 1 000 mg/kg/day groups.
Haematology:
Slight decreases in mean erythrocyte count and haematocrit at all treatment doses and in
haemoglobin and mean cell volume at 1 000 mg/kg/day in both sexes. However, these
changes were only significant in females.
Significantly increased mean cell haemoglobin concentration in females of the 100 and
1 000 mg/kg/day group and in males of the 1 000 mg/kg/day group.
FULL PUBLIC REPORT 26 April 2000
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