GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS
GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS
GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS
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that sensitisation may have been induced.<br />
To confirm these results a 2 nd challenge was conducted 7<br />
days later using the same test animals and challenge<br />
concentration. Ten non previously exposed naïve animals<br />
were also concurrently treated with the test substance.<br />
2 nd challenge - number of animals exhibiting positive responses:<br />
2 nd Test Animals<br />
Naïve Control Animals<br />
Challenge<br />
Concentration 24 hours T 48 hours T 24 hours T 48 hours T<br />
5%<br />
9*/19<br />
10**/19<br />
T time after patch removal.<br />
* number of animals exhibiting Grade +/- reactions.<br />
** number of animals exhibiting Grade 1 reactions.<br />
11*/19<br />
7**/19<br />
5*/10<br />
5**/10<br />
FULL PUBLIC REPORT 26 April 2000<br />
NA/713 Page 59 of 100<br />
3*/10<br />
7**/10<br />
Comment: Following rechallenge the incidence of Grade 1 reactions<br />
was similar between test and control animals. The study<br />
authors concluded that sensitisation had not been induced.<br />
Result: <strong>C16</strong>/C<strong>18</strong> Alpha olefins, isomerised was not considered to be<br />
sensitising to guineapig skin<br />
9.1.7.3 Skin Sensitisation of C20-24 Alkenes, branched and linear (Safepharm<br />
Laboratories Limited 1998)<br />
Test substance: C20-24 Alkenes, branched and linear<br />
Species/strain: Guineapig/Dunkin Hartley White<br />
Number of animals: 20 test females, 10 control females<br />
Test method: OECD TG 406 Magnusson and Kligman Maximisation<br />
Method<br />
Induction procedure: Test animals:<br />
Day 1: three pairs of intradermal injections (0.1 mL) into the<br />
dorsal skin of the scapular region:<br />
- Freund’s complete adjuvant (FCA) 1:1 in distilled<br />
water;<br />
- the test substance diluted to 25% w/v in arachis oil;<br />
- the test substance at 25% w/v emulsified in a 50:50<br />
mixture of FCA and distilled water;