GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS

that sensitisation may have been induced.
To confirm these results a 2 nd challenge was conducted 7
days later using the same test animals and challenge
concentration. Ten non previously exposed naïve animals
were also concurrently treated with the test substance.
2 nd challenge - number of animals exhibiting positive responses:
2 nd Test Animals
Naïve Control Animals
Challenge
Concentration 24 hours T 48 hours T 24 hours T 48 hours T
5%
9*/19
10**/19
T time after patch removal.
* number of animals exhibiting Grade +/- reactions.
** number of animals exhibiting Grade 1 reactions.
11*/19
7**/19
5*/10
5**/10
FULL PUBLIC REPORT 26 April 2000
NA/713 Page 59 of 100
3*/10
7**/10
Comment: Following rechallenge the incidence of Grade 1 reactions
was similar between test and control animals. The study
authors concluded that sensitisation had not been induced.
Result: C16/C18 Alpha olefins, isomerised was not considered to be
sensitising to guineapig skin
9.1.7.3 Skin Sensitisation of C20-24 Alkenes, branched and linear (Safepharm
Laboratories Limited 1998)
Test substance: C20-24 Alkenes, branched and linear
Species/strain: Guineapig/Dunkin Hartley White
Number of animals: 20 test females, 10 control females
Test method: OECD TG 406 Magnusson and Kligman Maximisation
Method
Induction procedure: Test animals:
Day 1: three pairs of intradermal injections (0.1 mL) into the
dorsal skin of the scapular region:
- Freund’s complete adjuvant (FCA) 1:1 in distilled
water;
- the test substance diluted to 25% w/v in arachis oil;
- the test substance at 25% w/v emulsified in a 50:50
mixture of FCA and distilled water;