GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS

9.1.7.2 Skin Sensitisation of C16/C18 Alpha olefins, isomerised (Hill Top Biolabs
Inc 1995)
Test substance: C16/C18 Alpha olefins, isomerised
Species/strain: Guineapig/Hartley White
Number of animals: 20 test animals
10 primary challenge naïve control animals;
10 rechallenge naïve control animals
Test method: OECD TG 406 - Buehler test
Induction procedure: Test animals:
Days 1, 7 and 14: 0.3 mL of neat test substance in mineral oil
applied, via a Hill Top Chamber, to the clipped skin of the
left shoulder for a 6 hour period;
Challenge procedure: 1 st challenge:
test and naïve control animals:
Day 28: same procedure as induction phase except 5% w/v
test substance in mineral oil was applied to a previously non
treated site.
2 nd challenge:
Day 35: same procedure as for 1 st challenge except
previously non exposed naïve animals were used.
Challenge outcome:
Grading of dermal responses occurred 24 and 48 hours post
exposure.
1 st challenge - number of animals exhibiting positive responses:
1 st Test Animals
Naïve Control Animals
Challenge
Concentration 24 hours T 48 hours T 24 hours T 48 hours T
5%
9*/19
10**/19
10*/19
9**/19
7*/10
3**/10
FULL PUBLIC REPORT 26 April 2000
NA/713 Page 58 of 100
8*/10
2**/10
T time after patch removal.
* number of animals exhibiting Grade +/- reaction, slight, patchy erythema.
** number of animals exhibiting Grade 1 reaction, slight but confluent, or moderate patchy erythema.
Comment: Test animals had a slightly greater incidence of Grade 1
reactions compared to the naïve control animals, suggesting