GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS
GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS
GULFTENE C16-18 ISOMERISED OLEFINS - NICNAS
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9.1.7.2 Skin Sensitisation of <strong>C16</strong>/C<strong>18</strong> Alpha olefins, isomerised (Hill Top Biolabs<br />
Inc 1995)<br />
Test substance: <strong>C16</strong>/C<strong>18</strong> Alpha olefins, isomerised<br />
Species/strain: Guineapig/Hartley White<br />
Number of animals: 20 test animals<br />
10 primary challenge naïve control animals;<br />
10 rechallenge naïve control animals<br />
Test method: OECD TG 406 - Buehler test<br />
Induction procedure: Test animals:<br />
Days 1, 7 and 14: 0.3 mL of neat test substance in mineral oil<br />
applied, via a Hill Top Chamber, to the clipped skin of the<br />
left shoulder for a 6 hour period;<br />
Challenge procedure: 1 st challenge:<br />
test and naïve control animals:<br />
Day 28: same procedure as induction phase except 5% w/v<br />
test substance in mineral oil was applied to a previously non<br />
treated site.<br />
2 nd challenge:<br />
Day 35: same procedure as for 1 st challenge except<br />
previously non exposed naïve animals were used.<br />
Challenge outcome:<br />
Grading of dermal responses occurred 24 and 48 hours post<br />
exposure.<br />
1 st challenge - number of animals exhibiting positive responses:<br />
1 st Test Animals<br />
Naïve Control Animals<br />
Challenge<br />
Concentration 24 hours T 48 hours T 24 hours T 48 hours T<br />
5%<br />
9*/19<br />
10**/19<br />
10*/19<br />
9**/19<br />
7*/10<br />
3**/10<br />
FULL PUBLIC REPORT 26 April 2000<br />
NA/713 Page 58 of 100<br />
8*/10<br />
2**/10<br />
T time after patch removal.<br />
* number of animals exhibiting Grade +/- reaction, slight, patchy erythema.<br />
** number of animals exhibiting Grade 1 reaction, slight but confluent, or moderate patchy erythema.<br />
Comment: Test animals had a slightly greater incidence of Grade 1<br />
reactions compared to the naïve control animals, suggesting