The Geography of Phytochemical Races

104 2 Examples Within Continents
covering extensive areas in Alberta, British Columbia, eastern Washington, Idaho,
western Montana, northeastern Oregon, Utah, and northern Arizona, although the range
is interrupted in places. Taxonomic diffi culties with this species can be appreciated by
refl ecting on the fact that no fewer than 23 synonyms for L. sericeus are recorded in
Vascular Plants of the Pacifi c Northwest (Hitchcock et al., 1961), and that Fleak (1971)
studied 61 taxa thought by some workers to belong to the L. sericeus complex.
Following an extensive study of lupines in western North America (Nicholls
and Bohm, 1982a), a detailed study of L. sericeus was undertaken (Nicholls and
Bohm, 1982b). Visual comparisons of two-dimensional thin layer chromatograms
representing 181 individual plants from 32 populations covering the entire range of
the species (Fig. 2.65) showed major differences in size of the spot corresponding
to orientin (8-C-glucosylluteolin) [213, R 1 = hydrogen, R 2 = glucose] (see Fig. 2.66
for structures 213 and 214). High-performance liquid chromatography (HPLC)
Fig. 2.65 Map showing the
collection sites for Lupinus
sericeus study
Fig. 2.66 Compounds 213 and 214, key fl avonoids from Lupinus sericeus study