CHEMOMETRICS ASSISTED UV-SPECTROPHOTOMETRIC ...

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30 25 20 15 10 5 0 -5 -10 -15 -20 200 215 230 245 260 275 290 305 320 335 350 365 380 395 Fig.31. First derivative spectra of resorcinol at different concentration levels ( 5-50 µg/ml ) 0.4 0.3 0.2 0.1 0.0 -0.1 -0.2 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 37 RE 35mcg SA 35mcg Fig.32. First derivative spectra for salicylic acid (35 µg/ml) resorcinol (35 µg/ml), indicating the zero - crossing points of each of them By taking the zero-crossing points of resorcinol for the estimation of salicylic acid the following analytical parameters and predicted values were obtained (Table 4 and Table 5). The same procedure was followed for determination of resorcinol in the presence of salicylic acid and the results of it are presented in Table 4 and Table 6.
Compound λ * (nm) Salicylic acid Resorcinol Table 4. Spectral and analytical parameters for both salicylic acid and resorcinol as calculated from the first derivative zero crossing points against the given concentrations of standard compounds at the selected wavelengths: 290.5 nm 317.5 nm 297.5 nm LC R ** (µg/ml) 5-50 5-50 5-50 Slope (b) +SE*** -0.00019+0.00031 0.00124+0.00069 0.00047+0.00106 38 Intercept (a) +SE 0.00068+0.00001 -0.00079+0.00002 -0.00180+0.00003 r * r 2 ** LOD † (µg/ ml) 0.9991 0.998 1.4 0.9968 0.9986 * λ=wavelength of determination, ** LCR= linear calibration range, *** SE=standard error , * r= correlation coefficient ** r 2 = determination coefficient,† LOD= limit of detection, †† LOQ= limit of quantification 0.994 0.997 2.6 1.7 LOQ †† (µg/ ml) 4.6 8.7 5.8
Page 1 and 2: ADDIS ABABA UNIVERSITY SCHOOL OF GR
Page 3 and 4: ADDIS ABABA UNIVERSITY SCHOOL OF GR
Page 5 and 6: ACKNOWLEDGEMENTS Above all, my pric
Page 7 and 8: 3.4.2. Preparation of samples......
Page 9 and 10: LIST OF TABLES Table 1. Spectral an
Page 11 and 12: LIST OF FIGURES Fig.1. How chemomet
Page 13 and 14: ABSTRACT Chemometrics-assisted spec
Page 15 and 16: Chemometric is the chemical discipl
Page 17 and 18: Y = a+bx.................. [2] wher
Page 19 and 20: from large sets of data. Using mult
Page 21 and 22: Solving the equations for the K mat
Page 23 and 24: • Calibrations are generally more
Page 25 and 26: The advent of rapid, in expensive c
Page 27 and 28: The derivative spectra are always m
Page 29 and 30: The method is based on the use of t
Page 31 and 32: 2. OBJECTIVES 2.1. General objectiv
Page 33 and 34: 3.4.2. Preparation of samples A. Wh
Page 35 and 36: drug (see the discussion section).
Page 37 and 38: 3 2.5 2 1.5 1 0.5 0 200 215 230 245
Page 39 and 40: Concentration (µg/ml) Fig.15. The
Page 41 and 42: Fig.20. Box plot for pure resorcino
Page 43 and 44: It is obvious from the figures that
Page 45 and 46: From the calculated degree of overl
Page 47 and 48: Compound λ* (nm) Table 1. Spectral
Page 49: Table 3. Actual and predicted amoun
Page 53 and 54: Table 6. Actual and predicted amoun
Page 55 and 56: The ratio spectra thus obtained are
Page 57 and 58: 0.1 0.0 0.0 0.0 -0.0 -0.0 -0.1 -0.1
Page 59 and 60: Table.8. Actual and predicted amoun
Page 61 and 62: absorbitivity and concentration eff
Page 63 and 64: Compound λ * (nm) Salicylic acid 2
Page 65 and 66: Table 12. Actual and predicted amou
Page 67 and 68: Dividing by C t, where C t is the t
Page 69 and 70: Table 13. Actual and predicted amou
Page 71 and 72: 4.5. Principal component regression
Page 73 and 74: Table 16. Predicted concentrations
Page 75 and 76: Component analyzed Pure benzoic aci
Page 77 and 78: Com pone nt anal yzed SP * Table 20
Page 79 and 80: 6. REFERENCES 1. Fifield, F.W. and
Page 81 and 82: 26. Danzer, K., Otto, M. and Currie

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