CHEMOMETRICS ASSISTED UV-SPECTROPHOTOMETRIC ...
CHEMOMETRICS ASSISTED UV-SPECTROPHOTOMETRIC ...
CHEMOMETRICS ASSISTED UV-SPECTROPHOTOMETRIC ...
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Compound λ *<br />
(nm)<br />
Salicylic<br />
acid<br />
Resorcinol<br />
Table 4. Spectral and analytical parameters for both salicylic acid and resorcinol as calculated from the first<br />
derivative zero crossing points against the given concentrations of standard compounds at the selected<br />
wavelengths:<br />
290.5 nm<br />
317.5 nm<br />
297.5 nm<br />
LC R **<br />
(µg/ml)<br />
5-50<br />
5-50<br />
5-50<br />
Slope (b)<br />
+SE***<br />
-0.00019+0.00031<br />
0.00124+0.00069<br />
0.00047+0.00106<br />
38<br />
Intercept<br />
(a) +SE<br />
0.00068+0.00001<br />
-0.00079+0.00002<br />
-0.00180+0.00003<br />
r * r 2 ** LOD<br />
†<br />
(µg/<br />
ml)<br />
0.9991 0.998 1.4<br />
0.9968<br />
0.9986<br />
* λ=wavelength of determination, ** LCR= linear calibration range, *** SE=standard error , * r= correlation<br />
coefficient<br />
** r 2 = determination coefficient,† LOD= limit of detection, †† LOQ= limit of quantification<br />
0.994<br />
0.997<br />
2.6<br />
1.7<br />
LOQ<br />
††<br />
(µg/<br />
ml)<br />
4.6<br />
8.7<br />
5.8