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CHEMOMETRICS ASSISTED UV-SPECTROPHOTOMETRIC ...

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Compound λ*<br />

(nm)<br />

Table 1. Spectral and analytical parameters for both benzoic acid and salicylic acid as calculated from the 1 D<br />

zero-crossing points against the given concentrations of standard compounds at the selected wavelengths:<br />

LC R **<br />

(µg/ml)<br />

Benzoic acid 237.5 5-50<br />

Salcylic acid 310.5 5-50<br />

Slope (b)<br />

+SE***<br />

0.00058+0.00252<br />

Intercept<br />

(a) +SE<br />

34<br />

r * r 2 ** LOD†<br />

(µg/ml<br />

)<br />

LOQ††<br />

(µg/ml)<br />

-0.00421+0.00008 0.9985 0.997 1.8 6.0<br />

-0.00029+0.00045 -0.00107+0.00002 0.9993 0.999 1.3 4.2<br />

* λ=wavelength of determination, ** LCR= linear calibration range, *** SE=standard error, * r=<br />

correlation coefficient<br />

** r 2 = determination coefficient,† LOD= limit of detection, †† LOQ= limit of quantification

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