CHEMOMETRICS ASSISTED UV-SPECTROPHOTOMETRIC ...

To sum the above mentioned explanation, other chemometricians believe that the
chemometric basically lie on-collecting and extracting maximum (optimum) information.
Using chemometrics method so many studies were conducted and reported in pharmaceutical
science, water quality assessment, sediment-quality analysis, forssenic discrimination,
biochemistry, and agricultural chemistry etc. using chemometric assisted techniques [15-19].
1.2. Multivariate analysis
Multivariate analysis is synonymous with the term chemometric [10]. It is a method which
takes into consideration many variables acting together. The method is fast and efficient in
determination as well as extraction of information [20- 22]. Multivariate analysis, as the
name suggests, involves looking simultaneously at the relationship between multiple data
values, rather than at individual ones [23].
1.2.1. Multivariate calibration
Chemometrics give emphasis on calibration [24]. In calibration the relationship between
signals (e.g. absorbance) and mass (or concentration) is set and established very well. The
well established relationship, of known concentrations, enables to extrapolate and converts
the signals observed (responses) to concentration (for the unknown one). This operation is
vital in the analytical laboratories [25, 26].
The term calibration can be defined as the use of empirical data and prior knowledge for
determining how to predict unknown quantitative information Y from available
measurements X, via a mathematical transfer function. Calibration hence is described as the
process of establishing this mathematical function (f) between measured variable x and a
dependent variable.
Y: f(x) = y................ [1]
One of the simplest forms of calibration is linear regression expression.
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