CHEMOMETRICS ASSISTED UV-SPECTROPHOTOMETRIC ...
CHEMOMETRICS ASSISTED UV-SPECTROPHOTOMETRIC ...
CHEMOMETRICS ASSISTED UV-SPECTROPHOTOMETRIC ...
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To sum the above mentioned explanation, other chemometricians believe that the<br />
chemometric basically lie on-collecting and extracting maximum (optimum) information.<br />
Using chemometrics method so many studies were conducted and reported in pharmaceutical<br />
science, water quality assessment, sediment-quality analysis, forssenic discrimination,<br />
biochemistry, and agricultural chemistry etc. using chemometric assisted techniques [15-19].<br />
1.2. Multivariate analysis<br />
Multivariate analysis is synonymous with the term chemometric [10]. It is a method which<br />
takes into consideration many variables acting together. The method is fast and efficient in<br />
determination as well as extraction of information [20- 22]. Multivariate analysis, as the<br />
name suggests, involves looking simultaneously at the relationship between multiple data<br />
values, rather than at individual ones [23].<br />
1.2.1. Multivariate calibration<br />
Chemometrics give emphasis on calibration [24]. In calibration the relationship between<br />
signals (e.g. absorbance) and mass (or concentration) is set and established very well. The<br />
well established relationship, of known concentrations, enables to extrapolate and converts<br />
the signals observed (responses) to concentration (for the unknown one). This operation is<br />
vital in the analytical laboratories [25, 26].<br />
The term calibration can be defined as the use of empirical data and prior knowledge for<br />
determining how to predict unknown quantitative information Y from available<br />
measurements X, via a mathematical transfer function. Calibration hence is described as the<br />
process of establishing this mathematical function (f) between measured variable x and a<br />
dependent variable.<br />
Y: f(x) = y................ [1]<br />
One of the simplest forms of calibration is linear regression expression.<br />
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