Book of Abstracts (PDF) - International Mycological Association
Book of Abstracts (PDF) - International Mycological Association
Book of Abstracts (PDF) - International Mycological Association
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IMC7 Main Congress Theme V: CELL BIOLOGY AND PHYSIOLOGY Posters<br />
1128 - Nuclear NAD metabolism in fungi and yeast<br />
A.M. Kerbalaeva * , S.M. Nasmetova, D.M. Ruzieva & T.G.<br />
Gulyamova<br />
Institute <strong>of</strong> Microbiology AS RU, A.Kadiry 7 B, Tashkent-<br />
700128, Uzbekistan. - E-mail: imbasru@uzsci.net<br />
Activity <strong>of</strong> NAD metabolism enzymes in nuclei at<br />
stimulation <strong>of</strong> NAD production by F. sambucinum and S.<br />
cerevisiae selected earlier as NAD-producers has been<br />
studied. Cultivation <strong>of</strong> these fungi and yeast in the medium<br />
containing 1 mM nicotinamide was accompanied by up to<br />
3-fold increasing <strong>of</strong> intracellular NAD concentration in<br />
steady state growth phase. The increasing <strong>of</strong> intracellular<br />
NAD concentration accompanied by falling <strong>of</strong> its<br />
intranuclear pool caused by acceleration <strong>of</strong> coenzyme<br />
turnover due by growing up <strong>of</strong> nuclear NADpyrophosphorylase<br />
and NAD-ase activites. Also we found<br />
that the decreasing <strong>of</strong> nuclear concentration <strong>of</strong> NAD during<br />
stimulation <strong>of</strong> its synthesis in cells by nicotinamide<br />
correlates with its utilization at ADP-riboslylation <strong>of</strong><br />
nuclear proteins. It was detected that increasing <strong>of</strong> basal<br />
and total ADP-ribosyl transferase activity attendant with<br />
appropriate changes in the level <strong>of</strong> modification <strong>of</strong> nuclear<br />
proreins as determined by the incorporation <strong>of</strong> 14 C -adenine<br />
from NAD into fungi and yeast nuclei. Moreover, as it was<br />
established by fractionation <strong>of</strong> nuclear proteins, although<br />
80% radiolabel is localized in the fraction <strong>of</strong> histones, the<br />
NAD content fluctuations mostly influences on the level <strong>of</strong><br />
ADP-ribosylation <strong>of</strong> nonhistone proteins. The conclusion<br />
was made that ADP-rybosylation <strong>of</strong> nuclear proteins might<br />
be one <strong>of</strong> the possible mechanisms <strong>of</strong> NAD oversynthesis<br />
regulation in fungi and yeast.<br />
1129 - Alkaline-tolerant fungi from Thailand: a source<br />
for alkaline enzymes?<br />
W. Kladwang 1* , A. Bhumiratana 2 , N.L. Hywel-Jones 1 & L.<br />
Lange 3<br />
1 BIOTEC, 73/1 Rama VI Rd. Radjhevee, Bangkok,<br />
Thailand. - 2 Mahidol University, Rama VI Rd, Rajdhevee,<br />
Bangkok, Thailand. - 3 Novozymes, Bagsvaerd 2880,<br />
Denmark. - E-mail: wipapat@biotec.or.th<br />
A collection <strong>of</strong> 490 isolates <strong>of</strong> alkaline-tolerant fungi was<br />
established using PDA buffered at pH11. This was from<br />
sand, soil, tree-holes and assorted plant material collected<br />
from southern, central and northern Thailand. If possible<br />
the sample pH was recorded and was between pH4 and 9.<br />
Although alkaline habitats were good sources <strong>of</strong> alkalinetolerant<br />
fungi it was significant that alkaline-tolerant fungi<br />
could be isolated from samples that were pH4. Strains were<br />
screened for their ability to hydrolyze arabinan, amylose,<br />
potato-galactan and skimmed milk at pH10. Eleven genera<br />
had activity against at least one <strong>of</strong> these substrates. Of<br />
these 11, 7 were members <strong>of</strong> the Hypocreales.<br />
Furthermore, <strong>of</strong> 57 positives, 51 belonged to the<br />
Hypocreales and 38 were Acremonium/Stilbella spp.<br />
342<br />
<strong>Book</strong> <strong>of</strong> <strong>Abstracts</strong><br />
Acremonium was therefore selected for further study.<br />
Alkaline enzyme production was tested using 9 substrates.<br />
Four different groups <strong>of</strong> Acremonium were separated based<br />
on their enzyme production pr<strong>of</strong>iles. From these, isolate<br />
WK276 (neutral rock-hole, south <strong>of</strong> Thailand) was selected<br />
for further study. Of the nine enzymes, the α-amylase<br />
properties <strong>of</strong> isolate WK276 were characterised. The Nterminal<br />
<strong>of</strong> WK276 α-amylase was studied for developing<br />
a specific primer. This was used to determine the complete<br />
sequence coding for the α-amylase.<br />
1130 - A time course study <strong>of</strong> functional and structural<br />
differences between two Glomus mosseae isolates<br />
L.M. Knudsen 1* , S. Dickson 2 , I. Jakobsen 1 & S.E. Smith 2<br />
1 Risoe National Laboratory, PO BOX 49, 4000 Roskilde,<br />
Denmark. - 2 Dept. <strong>of</strong> Soil and Water, University <strong>of</strong><br />
Adelaide, PMB 1, Glen Osmond, SA 5064, Australia. - Email:<br />
lisa.munkvold@risoe.dk<br />
A time course study was conducted with symbioses<br />
between Cucumis sativus and two Glomus mosseae isolates<br />
BEG161 and BEG29 previously shown to differ in hyphal<br />
growth pattern. Four consecutive harvests were employed<br />
to investigate plant growth and P uptake through time as<br />
well as the development <strong>of</strong> intra- and extraradical<br />
mycelium <strong>of</strong> the two fungi. Intraradical fungal structures<br />
were visualised by light microscopy and laser scanning<br />
confocal microscopy <strong>of</strong> NBT- and acid fuchsin-stained<br />
sections. Mycorrhizal cucumber plants grew better and<br />
reached higher P contents than non-mycorrhizal plants<br />
within 29 days. However, the magnitude <strong>of</strong> the mycorrhizal<br />
growth response depended on the fungal isolate. The rate<br />
<strong>of</strong> intraradical development differed between the two<br />
isolates, but reached the same final level. The most rapid<br />
colonisation occurred in the time intervals 14-23 days and<br />
23-29 days for BEG161 and BEG29, respectively. The<br />
structure <strong>of</strong> the intraradical mycelium was similar, but<br />
arbuscular branching patterns generally seemed very<br />
variable. Finally, the present data show that cross walls, as<br />
reported by Dickson & Smith (2001), are also formed in<br />
arbuscular trunk hyphae <strong>of</strong> G. mosseae colonising C.<br />
sativus.<br />
1131 - The pecularieties <strong>of</strong> laccases biosynthesis by cocultivated<br />
white-rot fungi Cerrena maxima and Coriolus<br />
hirsutus<br />
O.V. Koroleva 1 , V.P. Gavrilova 2* , E.V. Stepanova 1 , I.<br />
Yavmetdinov 1 & A. Antipov 1<br />
1 A.N.Bach Institute <strong>of</strong> Biochemistry Russian Academy <strong>of</strong><br />
Sciences, Leninskii pr.33, Moscow, 117071, Russia. -<br />
2 V.L.Komarov Institute <strong>of</strong> Botany Russian Academy <strong>of</strong><br />
Sciences, Pr<strong>of</strong>. Popova 2, St. Petersburg, 198022, Russia. -<br />
E-mail: Valeria@VG2438.spb.edu