Investigating CSI – Background material Table of Contents I ...
Investigating CSI – Background material Table of Contents I ...
Investigating CSI – Background material Table of Contents I ...
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Y-Chromosome Analysis (Y-STR Haplotypes)<br />
Y-chromosome haplotypes are used to uniquely identify men. Except for two<br />
small regions in which pairing and exchange take place with the X chromosome,<br />
the Y-chromosome is not subject to recombination and is passed on through the<br />
male line <strong>of</strong> the family largely unchanged. This allows a common paternal link to<br />
be traced in their ancestral lineage. By looking at Y-chromosome markers from<br />
all <strong>of</strong> the males with the same paternal descent we would suspect that all related<br />
males should share the same Y-chromosome markers. Therefore, identical Y<br />
STR patterns indicate inclusion within a family, whereas different Y STR patterns<br />
indicate familial exclusion.<br />
The figure below shows how the Y-chromosome (black squares) is passed from<br />
father to son exclusively.<br />
Testing Methodology<br />
Synergene utilises the PowerPlex Y STR kit (Promega), which allows analysis <strong>of</strong><br />
12 Y STR loci. Results from this analysis are compared to a population Y<br />
haplotype database.<br />
Indications for Y STR Analysis<br />
• To determine if males are from the same paternal lineage<br />
• To determine relatedness from father’s side <strong>of</strong> the family<br />
• To reconstruct family paternal-linked relationships<br />
• In sexual Assault cases, to distinguish the male sample from mixed<br />
samples: Source: www.synergenepr<strong>of</strong>iling.com<br />
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