Investigating CSI – Background material Table of Contents I ...

Recommendations

Info

For example, in the first loci (which spans the region from 100-150 base pairs) the victim could have 15 sequence repeats from her mother and 16 from her father, while, the suspect could have 20 repeats from his mother and 18 from his father. The PCR reaction with the fluorescein-labeled primers would have attached tags onto each length of repeats. When the sample is run through electrophoresis, the fluorescein tag would be illuminated, and a reading would be taken. As we know, smaller samples run faster through electrophoresis, thus the lane containing the victim’s DNA (which contains some 15-repeat segments and some 16-repeat segments) would fluoresce and give a reading before the lane containing the suspect’s DNA (containing 18- and 20-repeat segments). The victim’s results would show two peaks for the first loci, one at 15 and one at 16. The suspect’s results would also show two peaks for the first loci, but they would be at 18 and 20. Each of the five loci shown above would contain two peaks for both the victim’s results and the suspect’s results. …………………………………………………………………. 100 120 140 160 15 16 ………………………………………………………………….. 100 120 140 160 18 52 20 Victim’s results Suspect’s results
The other two PowerPlex16 groups of loci: The JOE-labeled allelic ladder components The TMR-labeled allelic ladder components 53
Page 1 and 2: Investigating CSI - Background mate
Page 3 and 4: convict the killer of Gill Dando wa
Page 5 and 6: A forensic scientists needs a honou
Page 7 and 8: lond, is also wearing Air Jordans w
Page 9 and 10: of unidentified remains with the pr
Page 11 and 12: Matching DNA Proving that a suspect
Page 13 and 14: Creating a DNA Profile: The Basics
Page 15 and 16: with thymine and guanine with cytos
Page 17 and 18: power is the combined analysis of a
Page 19 and 20: of DNA profile comparisons done by
Page 21 and 22: II. Tools and Techniques for DNA Fo
Page 23 and 24: p30 Test Unlike the presumptive aci
Page 25 and 26: Specificity refers to how many othe
Page 27 and 28: expected to occur when using your c
Page 29 and 30: helps to bind oxygen as well.) When
Page 31 and 32: chemical reaction, but in order to
Page 33 and 34: 2. Brauner, P.& Gallili, N., "A Con
Page 35 and 36: DNA Extraction Techniques Organic-b
Page 37 and 38: C. Quantification of DNA Southern B
Page 39 and 40: Southern Up Close In this simplifie
Page 41 and 42: 2. cDNA is denatured at more than 9
Page 43 and 44: SYBR-green SYBR green is another me
Page 45 and 46: Let's look at two people and the se
Page 47 and 48: at this locus and a different sized
Page 49 and 50: These loci are located on the same
Page 51: Short Tandem Repeat (STR) Analysis
Page 55 and 56: The Federal Bureau of Investigation
Page 57 and 58: eference sample from the standard s
Page 59 and 60: III. Interesting Cases Using DNA Ev
Page 61 and 62: me. But I was also lucky that when
Page 63 and 64: The tests indicated that the drops
Page 65 and 66: July 17, 1918. Czar Nicholas II, hi
Page 67 and 68: Guest list pared With the refusal o
Page 69 and 70: But only 18 of the states are hooke
Page 71: that to all felony offenses, as Ala

Investigating CSI – Background material Table of Contents I ...

Create successful ePaper yourself

Delete template?

Save as template?