Investigating CSI – Background material Table of Contents I ...
Investigating CSI – Background material Table of Contents I ...
Investigating CSI – Background material Table of Contents I ...
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C. Quantification <strong>of</strong> DNA<br />
Southern Blot<br />
The Southern blot is used to detect the presence <strong>of</strong> a particular bit <strong>of</strong> DNA in a<br />
sample. The DNA detected can be a single gene, or it can be part <strong>of</strong> a larger<br />
piece <strong>of</strong> DNA such as a viral genome.<br />
DNA is extracted from the cells and purified<br />
Restriction Digest<br />
DNA is restricted (cut) with enzymes.<br />
DNA fragments<br />
In this example, a large piece <strong>of</strong> DNA is chopped into<br />
smaller pieces using a restriction enzyme.<br />
Loading the Gel<br />
The DNA is loaded into a well <strong>of</strong> the gel matrix.<br />
• Lane 1 contains size standards (a mix <strong>of</strong> known DNA<br />
fragments)<br />
• Lane 2 contains the restricted DNA<br />
• Lane 3 contains unrestricted (whole) DNA<br />
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