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Investigating CSI – Background material Table of Contents I ...

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• purify DNA from any type <strong>of</strong> agarose;<br />

• concentrate DNA (for changing buffer, desalting);<br />

• remove proteins (after restriction enzyme treatment; dephosporylation with<br />

BAP or CIAP);<br />

• remove unincorporated nucleotides, primer, primer-dimers and enzymes<br />

from a labeling reaction or PCR;<br />

• remove an excess <strong>of</strong> linkers after ligation;<br />

• remove residual phenol, chlor<strong>of</strong>orm, and ethidium bromide.<br />

• purify plasmid DNA free <strong>of</strong> RNA from bacterial lysates.<br />

Source: http://herpesvirus.tripod.com/research/protoDNA.htm<br />

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