Investigating CSI – Background material Table of Contents I ...
Investigating CSI – Background material Table of Contents I ...
Investigating CSI – Background material Table of Contents I ...
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• purify DNA from any type <strong>of</strong> agarose;<br />
• concentrate DNA (for changing buffer, desalting);<br />
• remove proteins (after restriction enzyme treatment; dephosporylation with<br />
BAP or CIAP);<br />
• remove unincorporated nucleotides, primer, primer-dimers and enzymes<br />
from a labeling reaction or PCR;<br />
• remove an excess <strong>of</strong> linkers after ligation;<br />
• remove residual phenol, chlor<strong>of</strong>orm, and ethidium bromide.<br />
• purify plasmid DNA free <strong>of</strong> RNA from bacterial lysates.<br />
Source: http://herpesvirus.tripod.com/research/protoDNA.htm<br />
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