R & F® Salmonella (Nontyphoidal) Chromogenic Plating Medium
R & F® Salmonella (Nontyphoidal) Chromogenic Plating Medium
R & F® Salmonella (Nontyphoidal) Chromogenic Plating Medium
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Catalog No. M-1300 U.S. Patent 7,150,977<br />
PRODUCTS<br />
R & <strong>F®</strong> <strong>Salmonella</strong> (<strong>Nontyphoidal</strong>)<br />
<strong>Chromogenic</strong> <strong>Plating</strong> <strong>Medium</strong><br />
A Selective/Differential <strong>Chromogenic</strong> <strong>Plating</strong> <strong>Medium</strong> For Identifying<br />
<strong>Salmonella</strong> Excluding <strong>Salmonella</strong> Typhi/Paratyphi<br />
Presumptive Positive Colonies of <strong>Salmonella</strong> appear as reddish-pink raised colonies 1.0 to 3.0 mm<br />
diameter with colorless ring after 20 - 24 hours incubation at 35°C<br />
<strong>Salmonella</strong><br />
<strong>Salmonella</strong> and Escherichia coli<br />
<strong>Salmonella</strong><br />
E. coli<br />
Advantages of R & <strong>F®</strong> <strong>Salmonella</strong> (<strong>Nontyphoidal</strong>) <strong>Chromogenic</strong> <strong>Plating</strong> <strong>Medium</strong><br />
• A highly selective chromogenic plating medium that isolates <strong>Salmonella</strong> sp. excluding <strong>Salmonella</strong><br />
Typhi/Paratyphi while either differentiating from or inhibiting the growth of many similar bacteria<br />
• High specificity and sensitivity; differentiates <strong>Salmonella</strong> and Citrobacter<br />
• Easily prepared from powdered plating medium and powdered supplement<br />
• Prepared plates are stable for at least 45 days in the dark at 2-8°C<br />
• Designed for testing food and environmental samples
Selective and Differential properties of R & <strong>F®</strong> <strong>Salmonella</strong> (<strong>Nontyphoidal</strong>) <strong>Chromogenic</strong> <strong>Plating</strong> <strong>Medium</strong><br />
incubated at 35°C for 24 hours<br />
Bacteria<br />
<strong>Salmonella</strong> sp.<br />
(subspp. I, II, IV, VI)<br />
<strong>Salmonella</strong> sp.<br />
(subspp. IIIa and IIIb)<br />
(Arizona)<br />
Number<br />
of<br />
Strains<br />
<strong>Salmonella</strong> Typhi/Paratyphi 4 No Growth<br />
<strong>Salmonella</strong> bongori<br />
(Subspp. V)<br />
60<br />
16<br />
Colonial Morphology<br />
Reddish-pink raised colonies; 1.0-3.0 mm diameter with/without pink precipitate or colorless to<br />
light pink ring around colony<br />
Reddish-pink raised colonies; 1.0-3.0 mm diameter with/without pink precipitate or colorless to<br />
light pink ring around colony<br />
2 Dark blue raised colony; 2.0-3.0 mm diameter with pink precipitate, but no colorless ring<br />
around colony<br />
Escherichia coli O157:H7 12 Blue-green domed to raised colony; 1.0-3.0 mm diameter with green precipitate and no ring<br />
Escherichia coli 14 Blue-green domed to raised colony; 2.0-4.0 mm diameter with blue-green precipitate and no<br />
colorless ring around colony<br />
Escherichia coli 4 Dark blue domed to raised colony; 2.0-3.0 mm diameter with pink precipitate and no ring<br />
Escherichia coli 1 Red-pink raised colony; 2.0 mm diameter with pink precipitate and thin colorless ring<br />
Escherichia hermannii 2 Colorless pinpoint colonies<br />
Citrobacter diversus 2 Greenish-blue domed to raised pinpoint colony to 1.0 mm diameter and no precipitate<br />
Citrobacter freundii 15 Dark blue to blue-green domed colony; 1.0 mm diameter with no precipitate or ring<br />
Serratia marcescens 1 Light green domed colony; 2.0 mm diameter with no precipitate<br />
Hafnia alvei 4 Blue-green domed colony; pinpoint to
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