Use of nasal and buccal cells in human biomonitoring ... - Formacare
Use of nasal and buccal cells in human biomonitoring ... - Formacare
Use of nasal and buccal cells in human biomonitoring ... - Formacare
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Micronucleus frequencies <strong>in</strong> exfoliated <strong>nasal</strong> <strong>cells</strong> <strong>of</strong> <strong>human</strong> subjects after<br />
formaldehyde exposure<br />
Study size (subjects/<strong>cells</strong><br />
scored)<br />
15 Exposed/15 controls<br />
6000 <strong>cells</strong>/subject<br />
29 Pre- <strong>and</strong> post exposure<br />
1500 <strong>cells</strong>/subject<br />
28 Pre- <strong>and</strong> post exposure<br />
500–4000 <strong>cells</strong>/per subject<br />
23 Exposed/25 controls<br />
3000 <strong>cells</strong>/subject<br />
18 Exposed/23 controls<br />
3000 <strong>cells</strong>/subject<br />
16 Exposed/23 controls<br />
????? <strong>cells</strong>/subject<br />
41 Exposed/the same subject were<br />
controls 1000-2000 cell subject<br />
Exposure<br />
(ppm)<br />
siegfried.knasmueller@meduniwien.ac.at<br />
Sta<strong>in</strong><strong>in</strong>g<br />
0.1–0.39 Feulgen/Fast green<br />
1.4 (peak:<br />
6.6)<br />
1.4 (peak:<br />
6.6)<br />
Feulgen/Fast green<br />
FISH/propidium<br />
iodide<br />
2–4 Feulgen/Fast green<br />
1 Wright's sta<strong>in</strong><br />
0.1 Wright's sta<strong>in</strong><br />
0 to 0.7<br />
Result: <strong>nasal</strong> <strong>cells</strong><br />
(exposed (‰)/controls<br />
(‰))<br />
0.90/0.25<br />
↑ 3.6<br />
0.5/0.41<br />
↔<br />
2.5/2.0<br />
↔<br />
1.0/0.61<br />
↑ 1.6<br />
2.70/1.25<br />
↑ 2.2<br />
3.9/1.2<br />
↑ 3.3<br />
DAPI 0.21/0.17<br />
↔<br />
Ref. Notes<br />
Ballar<strong>in</strong> et al.,<br />
1992<br />
Suruda et al.,<br />
1993<br />
Titenko-Holl<strong>and</strong><br />
et al., 1996<br />
Burgaz et al.,<br />
2001<br />
Ye et al., 2005<br />
Y<strong>in</strong>g et al., 1997<br />
Zeller et al.,<br />
2011<br />
-<br />
13% <strong>in</strong>crease <strong>in</strong><br />
males, 37% <strong>in</strong><br />
females<br />
First study <strong>of</strong> <strong>nasal</strong><br />
<strong>cells</strong> with FISH<br />
technique<br />
-<br />
Not reliable results<br />
because <strong>of</strong> sta<strong>in</strong><br />
Not reliable results<br />
because <strong>of</strong> sta<strong>in</strong><br />
Exposure under<br />
controlled<br />
conditions 33
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