Use of nasal and buccal cells in human biomonitoring ... - Formacare
Use of nasal and buccal cells in human biomonitoring ... - Formacare
Use of nasal and buccal cells in human biomonitoring ... - Formacare
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Micronucleus frequencies <strong>in</strong> exfoliated <strong>buccal</strong> <strong>cells</strong> <strong>of</strong> <strong>human</strong> subjects after<br />
formaldehyde exposure<br />
Study size (subjects/<strong>cells</strong> scored) Exposur<br />
e (ppm)<br />
29 Pre- <strong>and</strong> post exposure<br />
1500 <strong>cells</strong>/subject<br />
28 Pre- <strong>and</strong> post exposure<br />
500–4000 <strong>cells</strong>/per subject<br />
28 Exposed/18 controls<br />
3000 <strong>cells</strong>/subject<br />
80 Exposed/85<br />
Controls<br />
2000 <strong>cells</strong>/subject<br />
16 Exposed/23 controls<br />
????? <strong>cells</strong>/subject<br />
56 Exposed/85 controls<br />
2000 <strong>cells</strong>/subject<br />
21 Pre- <strong>and</strong> post exposure 1000 -<br />
2000 <strong>cells</strong>/subject<br />
1.4<br />
(peak:<br />
6.6)<br />
1.4<br />
(peak:<br />
6.6)<br />
siegfried.knasmueller@meduniwien.ac.at<br />
Sta<strong>in</strong><strong>in</strong>g Result: <strong>buccal</strong> <strong>cells</strong><br />
(exposed<br />
(‰)/controls (‰))<br />
Feulgen/Fast green 0.6/0.046<br />
↑ 1.6<br />
FISH/propidium<br />
iodide<br />
2.0/0.6<br />
↑ 3.3<br />
2–4 Feulgen /fast green 7.1/3.3<br />
↑ 2.2<br />
0.25 Feulgen/no<br />
counter sta<strong>in</strong><br />
1.27/0.13<br />
↑ 9.8<br />
0.64/0.13<br />
↑ 4.9<br />
0.1 Wright's sta<strong>in</strong> 0.86/0.57<br />
↔<br />
0.16 Feulgen/no<br />
counter sta<strong>in</strong><br />
0.96/0.16<br />
↑ 6.0<br />
13.5 DAPI 1.33/0.90<br />
↔<br />
Ref. Notes<br />
Suruda et al.,<br />
1993<br />
Titenko-<br />
Holl<strong>and</strong> et al.,<br />
1996<br />
Burgaz et al.,<br />
2002<br />
Viegas et al.,<br />
2010<br />
Y<strong>in</strong>g et al.,<br />
1997<br />
Ladeira et al.,<br />
2011<br />
Speit et al.,<br />
2007<br />
0 MN <strong>in</strong> 22 males,<br />
but 0.19‰ <strong>in</strong><br />
females<br />
First study <strong>of</strong> <strong>buccal</strong><br />
<strong>cells</strong> with FISH<br />
technique<br />
-<br />
-<br />
Not reliable results<br />
because <strong>of</strong> sta<strong>in</strong><br />
♀↑ 10.4/♂↑ 2.8<br />
Exposure under<br />
controlled<br />
conditions<br />
32
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