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Beyond IUI, IVF and ICSI - New developments in - eshre 2013

Beyond IUI, IVF and ICSI - New developments in - eshre 2013

Beyond IUI, IVF and ICSI - New developments in - eshre 2013

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The electron microscopic results<br />

<strong>in</strong> our study showed that the rate<br />

of acrosome reaction was time<br />

dependent, with a maximum of 5<br />

hours. However, the<br />

fertilization rate was the highest<br />

when the spermatozoa were<br />

<strong>in</strong>cubated for 3 hours.<br />

Although the acrosome<br />

reaction <strong>in</strong>creases with<br />

time there may be an<br />

<strong>in</strong>crease <strong>in</strong> chromat<strong>in</strong><br />

decondensation <strong>in</strong> the<br />

sperm head that adversely<br />

affects fertilization.<br />

Therefore, based on the<br />

results of this study it is<br />

recommended to allow a<br />

3 hour <strong>in</strong>cubation period<br />

for spermatozoa before<br />

<strong>ICSI</strong> to obta<strong>in</strong> the best<br />

fertilization rate.<br />

Page 91 of 127

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