Beyond IUI, IVF and ICSI - New developments in - eshre 2013

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5. Birefringence–polarisation microscopy Partial sperm head birefringence Indicates acrosome reaction Higher clin PR and IR compared with non-reacted sperm Gianaroli et al. 2010 No comparative p studies available 46% pregnancy rate Ghosh et al. 2012 Limited clinical application Promising but expensive Not well-known ICSI with immotile sperm First pregnancy reported in 1997 Kahraman et al. 1997 Literature reports large ranges Fertilization rate: range 3% - 76.4% Pregnancy rate: range 0% - 38% Large series reported Kovacic et al. 2006 47 cycles with immotile testicular sperm 66% fertilization rate 38.3% pregnancy rate ICSI with immotile sperm Discrepancies in literature may be caused by Overall time/effort spend for searching motile sperm Real immotility vs virtual immotility of individual spermatozoa Accurate observation of individual sperm Choice of the technique to select viable sperm EExpertise i of f the h embryologist b l i with i h each h of f the h techniques h i Ejaculated sperm versus testicular sperm Search/observation time Real immotility Fertilization rate Page 74 of 127
Conclusions Absolute asthenozoospermia = rare condition (1/5000) Different techniques to distinguish viable and non-viable immotile spermatozoa Complexity, reliability Time, cost Pros and Cons No prospective randomized trial available in literature Limited comparative studies Limited number of cycles Mixed motile/immotile sperm for ICSI within a cycle Success rate with immotile sperm remains lower than with motile sperm References Afzelius 1976, Science 193, 317-319 Aktan et al. 2004, Andrologia 36, 366-369 Baccetti et al. 2004, J Submicrosc Cytol Pathol 36, 333-339 Barros et al; 1997, Hum Reprod 12, 1227-1229 Casper et al. 1996, Fertil Steril 65, 972-976 Cayan et al. 2001, Fertil Steril 76, 612-614 De Oliveira et al. 2004, Hum Reprod 19, 262-265 Eliasson et al. 1977, N Engl g J Med 297, 1-6 Emiliani et al. 2000, Hum Reprod 15, 2371-2374 Ghosh et al. 2012, Andrologia xx, 1-5 Gianaroli et al. 2008, Fertil Steril 90, 104-112 Gianaroli et al. 2010, Fertil Steril 93, 807-813 Jeyendran et al. 1984, J Reprod Fertil 70, 219-228 Kahraman et al. 1997, Hum Reprod 12, 292-293 Kovacic et al. 2006, J Androl 27, 45-52 Lecomte et al. 1998, In “Male sterility and motility disorders” eds Hamamah et al., 65-78 References Liu et al. 1995, Hum Reprod 10, 2630-2636 Mangoli et al. 2011, Fertil Steril 95, 631-634 Montag et al. 1999, Andrologia 31, 49-53 Montag and Rink 2001, ESHRE PCC Embryology, 19-32 Nagy et al. 1995, Hum Reprod 10, 1123-1129 Ortega et al. 2011, Hum Reprod Update 17, 684-692 Soares et al. 2003, Rev Hosp Clin 58, 250-253 Tash and Means 1983, Biol Reprod 28, 75-104 Terriou et al. 2000, J Assist Reprod Genet 17, 194-199 Tournaye et al. 1993, Hum Reprod 8, 1475-1480 Tournaye et al. 1994, Hum Reprod 9, 1903-1908 Tournaye te al. 1996, Fertil Steril 66, 331-334 Tournaye et al. 1998, In “Modern ART in the 2000s” eds Ombelet et al., 157-162 Vandervorst et al. 1997, Hum Reprod 12, 2429-2433 Verheyen et al; 1997, Hum Reprod Update 3, 195-203 Yatsenko et al. 2010, J Androl 31, 34-44 Yovich et al. 1988, Fertil Steril 50, 179-181 Zeyneloglu et al. 2000, Hum Reprod 15, 853-856 Page 75 of 127
Page 1:
28th Annual Meeting of the European
Page 5 and 6:
Contents Course coordinators, cours
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Course coordinators Herman Tournaye
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Scientific programme Sperm selectio
Page 11 and 12:
ESHRE SIG Andrology, Istanbul, Turk
Page 13 and 14:
October 2001 - “A Physics Revelat
Page 15 and 16:
Polydimethylsiloxan and Sperm Survi
Page 17 and 18:
Microfluidic Sperm Isolation Reduce
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Micro-insemination Device 1. Remove
Page 21 and 22:
Selecting spermatozoa; potential ro
Page 23 and 24:
Fertilization Rate (%) 120 100 80 6
Page 25 and 26: Spermsep CS10 System Sperm Cell Sep
Page 27 and 28: TUNEL (%) 14% 90 80 70 60 50 40 30
Page 29 and 30: Cathode (- ve terminal) Anode (+ ve
Page 31 and 32: Hydrogen peroxide mediates DNA dama
Page 33 and 34: ESHRE 2012: Pre‐congress course 2
Page 35 and 36: Sperm morphology and ICSI The estab
Page 37 and 38: Motile Sperm Organellar Morphology
Page 39 and 40: Conclusions of the meta‐analysis
Page 41 and 42: Does the presence of sperm nuclear
Page 43 and 44: Basic sperm parameters Sperm DNA fr
Page 45 and 46: No relationship between chromosome
Page 47 and 48: Gynecology: Filippo Ubaldi Elena Ba
Page 49 and 50: Sperm selection: why? There is an i
Page 51 and 52: I- MACS -Annexine V for diagnostic
Page 53 and 54: Evaluation of HA-binding and sperm
Page 55 and 56: HA-bound sperm and morphology with
Page 57 and 58: III. HA and ICSI outcome: prospecti
Page 59 and 60: HA- clinical uses and limitations
Page 61 and 62: May the force be with you Using imm
Page 63 and 64: Sperm ultrastructure Centrosome cha
Page 65 and 66: Diagnostic semen analysis Three ba
Page 67 and 68: Ultrastructural defects Transmissio
Page 69 and 70: Necrozoospermia / low viability rat
Page 71 and 72: 1. Hypo-osmotic swelling test ICSI
Page 73 and 74: 4. Laser-assisted selection (LAISS)
Page 75: 5. Birefringence-polarisation micro
Page 79 and 80: Definition of Azoospermia: Absence
Page 81 and 82: Cases of aspermia Preventive measur
Page 83 and 84: The probability crypto azoospermic
Page 85 and 86: "The beauty and the beast" when the
Page 87 and 88: Fertilization rate after ICSI 1- Oo
Page 89 and 90: With (ICSI), zona pellucida penetra
Page 91 and 92: Sibling oocytes from each patient w
Page 93 and 94: The electron microscopic results in
Page 95 and 96: Ionophore treatment for oocyte acti
Page 97 and 98: The oocytes were suspended in 0.3M
Page 99 and 100: Table 3. Pregnancy rates and outcom
Page 101 and 102: References • Devroey P, Van Steir
Page 103 and 104: Longing for a girl: Gender selectio
Page 105 and 106: 1,1 1 0,9 0,8 0,7 0,6 Sex ratio imb
Page 107 and 108: PhD Thesis Michelle Duc, 1977 Retro
Page 109 and 110: Reasons for wanting a girl at GC
Page 111 and 112: Assessment of cervical mucus OOpaqu
Page 113 and 114: Study design • A reference group
Page 115 and 116: Partial data Reason n No post‐pre
Page 117 and 118: Percentage P of girls 70 60 50 40 3
Page 119 and 120: New aspects of this research • Fi
Page 121 and 122: Mark your calendar for the upcoming
Page 123 and 124: NOTES Page 121 of 127
Page 125 and 126: NOTES Page 123 of 127
Page 127 and 128:
NOTES Page 125 of 127
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NOTES Page 127 of 127
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Beyond IUI, IVF and ICSI - New developments in - eshre 2013

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