Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
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4. Laser-assisted selection (LAISS)<br />
First described by Aktan et al. 2004<br />
Pr<strong>in</strong>ciple: s<strong>in</strong>gle laser schot close to the tail tip<br />
Live sperm react by tail curl<strong>in</strong>g<br />
Dead sperm show no reaction<br />
Advantages<br />
No exposure to non-physiological media or toxic compounds<br />
Rapid procedure<br />
Easy <strong>in</strong>terpretation<br />
Disadvantage<br />
Expensive equipment (laser)<br />
4. Laser-assisted selection (LAISS)<br />
Risks?<br />
No damage, permeabilization of sperm membrane<br />
Montag et al. 1999<br />
No <strong>in</strong>crease <strong>in</strong> DNA fragmentation<br />
Montag <strong>and</strong> R<strong>in</strong>k 2001<br />
<strong>ICSI</strong> results<br />
Higher fertilization <strong>and</strong> cleavage rate than HOST<br />
Aktan et al . 2004<br />
• Fertilization rate 64.2% vs 46.5%<br />
• Cleavage rate 79.6% vs 51.4%<br />
Cl<strong>in</strong>ically applied <strong>in</strong> UZ Brussel s<strong>in</strong>ce 2010<br />
Not widely applied (expensive)<br />
4. Laser-assisted selection (LAISS)<br />
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