Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
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HA- cl<strong>in</strong>ical uses <strong>and</strong> limitations<br />
• No clear beneficial outcome after <strong>in</strong>jection of HA-bound<br />
sperm compared to st<strong>and</strong>ard <strong>ICSI</strong><br />
• Only (highly) motile sperm can b<strong>in</strong>d to HA- is this<br />
parameter sufficient to select best sperm?<br />
• Only 2 Italian papers found a positive correlation<br />
between P<strong>ICSI</strong> <strong>and</strong> ART outcome: effect of oocyte<br />
selection?<br />
Conclusions <strong>and</strong> discussion<br />
Potentially role for selection based on other membrane markers like fertil<strong>in</strong><br />
alpha (ADAM-1), beta (ADAM-2) or ADAM-3/ <strong>in</strong>fertility-associated sperm<br />
prote<strong>in</strong> (IASP) / Zeta-b<strong>in</strong>d<strong>in</strong>g prote<strong>in</strong> / 57 kDa prote<strong>in</strong><br />
Role of Hyaluronan b<strong>in</strong>d<strong>in</strong>g prote<strong>in</strong>-1 (HABP-1)<br />
Low cl<strong>in</strong>ical application of HA-b<strong>in</strong>d<strong>in</strong>g test <strong>and</strong> P<strong>ICSI</strong>, potential bias?<br />
No r<strong>and</strong>omized cl<strong>in</strong>ical trials (RCT) on HA-bond sperm outcome: time to<br />
proceed?<br />
Both systems (MACS/HA) only suitable for samples with relative high % motile<br />
sperm, not suitable for extreme OAT/ PESA/ TESE<br />
References list<br />
Said TM, L<strong>and</strong> JA. Effects of advanced selection methods on sperm quality <strong>and</strong> ART outcome: a<br />
systematic review. Hum Reprod Update. 2011 Nov-Dec;17(6):719-33.<br />
Yagci A, et al. Spermatozoa bound to solid state hyaluronic acid show chromat<strong>in</strong> structure with high<br />
DNA cha<strong>in</strong> <strong>in</strong>tegrity: an acrid<strong>in</strong>e orange fluorescence study. J Androl. 2010 Nov-Dec;31(6):566-72.<br />
Pr<strong>in</strong>osilova P et al. Selectivity of hyaluronic acid b<strong>in</strong>d<strong>in</strong>g for spermatozoa with normal Tygerberg<br />
strict morphology. Reprod Biomed Onl<strong>in</strong>e. 2009 Feb;18(2):177-83.<br />
Said TM et al. Utility of magnetic cell separation as a molecular sperm preparation technique. J<br />
Androl. 2008 Mar-Apr;29(2):134-42.<br />
Huszar G, et al. Fertility test<strong>in</strong>g <strong>and</strong> <strong>ICSI</strong> sperm selection by hyaluronic acid b<strong>in</strong>d<strong>in</strong>g: cl<strong>in</strong>ical<br />
<strong>and</strong> genetic aspects.Reprod Biomed Onl<strong>in</strong>e. 2007 May;14(5):650-63.<br />
Said TM et al. Selection of nonapoptotic spermatozoa as a new tool for enhanc<strong>in</strong>g assisted<br />
reproduction outcomes: an <strong>in</strong> vitro model.Biol Reprod. 2006 Mar;74(3):530-7.<br />
Said TM et al. Advantage of comb<strong>in</strong><strong>in</strong>g magnetic cell separation with sperm preparation<br />
techniques. Reprod Biomed Onl<strong>in</strong>e. 2005 Jun;10(6):740-6.<br />
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