Beyond IUI, IVF and ICSI - New developments in - eshre 2013

Zeta and HA-binding : evaluation of sperm integrity
Razavi et al. Andrologia 2010 42(1):13-9
Semen sample 77 patients
CMA3 (Protamine content improvement with both Zeta
and HA –binding)
Sperm chromatin dispersion test (SCD) (DNA integrity
improved, Zeta method better)
Papanicolau stain (improvement sperm morphology with
both methods)
Diagram for sperm selection using zeta
potential. Sperm suspension is pipetted into
a positively charged tube. Negatively
charged mature sperm (A) will adhere to the
tube, while non-mature sperm (B) will not
adhere and are discarded.
HA- bound sperm: DNA damage evaluated by acridine
orange fluorescence (AOF)
Yagci et al. J. Andrology 2010. 31(6):566-572
Green fluorescence (double-
strengs t DNA)
• Total fraction 54%
• HA-bound sperm (in spot) :
99%
Morphology of HA-bound sperm evaluated by strict
criteria
• The proportion of normal spermatozoa
was higher in HA-bound with a 3.04-fold
improvement (95% confidence limits:
1.9-4.7) in 37 teratozoospermic men
Reprod Biomed Online. 2009 Feb;18(2):177-83.
Selectivity of hyaluronic acid binding for spermatozoa with normal Tygerberg strict morphology.
Prinosilova P, Kruger T, Sati L, Ozkavukcu S, Vigue L, Kovanci E, Huszar G.
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