Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
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I- MACS –Annex<strong>in</strong>e V for diagnostic test<strong>in</strong>g (density<br />
gradient centrifugation <strong>and</strong> MACS)<br />
Apoptotic markers: activated Caspase 3 / mitochondrial<br />
membrane potential (MMP) / TUNEL<br />
Sperm fertilization potential <strong>in</strong> hamster oocyte pentration <strong>and</strong><br />
hamster <strong>ICSI</strong>-test<br />
MACS-Annex<strong>in</strong>e V negative sperm:<br />
higher % motile sperm<br />
lower % sperm apoptotic makers<br />
higher % oocyte penetration<br />
no difference <strong>in</strong> chromat<strong>in</strong>e decondensation after <strong>ICSI</strong><br />
Patients: 60 couples with<br />
unexpla<strong>in</strong>ed <strong>in</strong>fertility <strong>and</strong><br />
failed <strong>IUI</strong><br />
Said et al. Reprod. Biomed Onl<strong>in</strong>e 2005 10(6):740-6<br />
Said et al. Biol Reprod. 2006 74(3):530-7<br />
II. MACS –Annex<strong>in</strong>e V for diagnostic test<strong>in</strong>g (density<br />
gradient centrifugation <strong>and</strong> MACS)<br />
Increased % of sperm with<br />
positive hemizona assay.<br />
Conclusion: posible benefit of<br />
MACS <strong>in</strong> unexpal<strong>in</strong>ed fertility.<br />
No cl<strong>in</strong>ical outcome<br />
described<br />
Lee et al. Hum. Reprod. 2010 25 (4): 839-46<br />
Cl<strong>in</strong>ical uses <strong>and</strong> limitations<br />
Significant<br />
reduce of<br />
apoptotic<br />
markers<br />
• M<strong>in</strong>imal amount of sperm for effectively recovery with<br />
MACS-Annex<strong>in</strong>e V ?<br />
• Not clear whether suitable for <strong>IUI</strong> / <strong>IVF</strong> or only <strong>ICSI</strong><br />
• Contam<strong>in</strong>ation <strong>and</strong> recovery rates?<br />
• No cl<strong>in</strong>ical uses at present<br />
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