Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Sperm selection: why?<br />
There is an <strong>in</strong>creas<strong>in</strong>g need for non-<strong>in</strong>vasive biochemical<br />
markers to select normal <strong>and</strong> functional sperm <strong>in</strong> ART,<br />
especially for <strong>ICSI</strong><br />
Sperm selection for ART: how?<br />
“Positive” selection (mature<br />
sperm are selected from damaged<br />
sperm; e.g. b<strong>in</strong>d<strong>in</strong>g to HA-matrix):<br />
mature sperm is selected for ART,<br />
unbound/unselected sperm is<br />
discarded<br />
“Negative” selection<br />
(damaged sperm b<strong>in</strong>d to<br />
membrane receptors; e.g.<br />
Annex<strong>in</strong>e-V, FAS-lig<strong>and</strong>s, TNFreceptors):<br />
unbound sperm can<br />
be collected <strong>and</strong> used for ART<br />
Sperm membranes are highly polyunsaturated; they have<br />
a specific constitution <strong>and</strong> function: changes are necessary<br />
for acrosom reaction, ZP recognition <strong>and</strong> oocyte for fusion<br />
Phosphatidyl<br />
ser<strong>in</strong>e<br />
Page 47 of 127
Change language