Beyond IUI, IVF and ICSI - New developments in - eshre 2013

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Lesson from IMSI approach (1) Sperm quality may affect ICSI results in terms of embryo development (blastocyst formation) and clinical outcome. No clear evidences have been published yet (evidence‐ based medicine, prospective randomized studies, enough power, identification of a specific category of patients) about the real efficacy of IMSI approach. Lesson from IMSI approach (2) Moreover contradictory results have been recently found from different groups about the significance and the nature of the presence of vacuoles on sperm competence The presence of sperm nuclear defects assessed by high magnification microscopy did not directly translate to chromosomal breakage. abnormalities or presence of DNA We need to investigate better this aspect and try to find different aspects other than sperm morphology that can have an impact on ICSI outcome ‐Nagy et al., 1995 ‐Svalander et al., 1996 ‐Bartoov et al. 2001, 2002, 2003 ‐De Vos et al., 2003 ‐Virro, Virro, et al. 2004 004 ‐Berkovitz et al. 2006 ‐Vanderzwalmen et al., 2008 ‐Garolla et al., 2008 ‐Watanabe et al., 2009 ‐Souza Setti et al., 2010 ‐Balaban et al., 2011 ‐Said and Land, 2011 ‐Palermo et al., 2011 References Page 44 of 127
Gynecology: Filippo Ubaldi Elena Baroni Silvia Colamaria Maddalena Giuliani Fabrizio Fiorini Antonio Ciconte Silvia Venanzi Antonio Lore Fabio Sapienza Annalise Giallonardo Mauro Schimberni www.generaroma.it CLINICA VALLE GIULIA, Roma SALUS – ASI MEDICAL, Marostica GENERA UMBERTIDE, Perugia Embryology: Laura Rienzi Stefania Romano Laura Albricci Antonio Capalbo Roberta Maggiulli Benedetta Iussig Nicoletta Barnocchi Sara Fusco Federica Sanges Catello Scarica Elena Ievoli Page 45 of 127
Page 1: 28th Annual Meeting of the European
Page 5 and 6: Contents Course coordinators, cours
Page 7 and 8: Course coordinators Herman Tournaye
Page 9 and 10: Scientific programme Sperm selectio
Page 11 and 12: ESHRE SIG Andrology, Istanbul, Turk
Page 13 and 14: October 2001 - “A Physics Revelat
Page 15 and 16: Polydimethylsiloxan and Sperm Survi
Page 17 and 18: Microfluidic Sperm Isolation Reduce
Page 19 and 20: Micro-insemination Device 1. Remove
Page 21 and 22: Selecting spermatozoa; potential ro
Page 23 and 24: Fertilization Rate (%) 120 100 80 6
Page 25 and 26: Spermsep CS10 System Sperm Cell Sep
Page 27 and 28: TUNEL (%) 14% 90 80 70 60 50 40 30
Page 29 and 30: Cathode (- ve terminal) Anode (+ ve
Page 31 and 32: Hydrogen peroxide mediates DNA dama
Page 33 and 34: ESHRE 2012: Pre‐congress course 2
Page 35 and 36: Sperm morphology and ICSI The estab
Page 37 and 38: Motile Sperm Organellar Morphology
Page 39 and 40: Conclusions of the meta‐analysis
Page 41 and 42: Does the presence of sperm nuclear
Page 43 and 44: Basic sperm parameters Sperm DNA fr
Page 45: No relationship between chromosome
Page 49 and 50: Sperm selection: why? There is an i
Page 51 and 52: I- MACS -Annexine V for diagnostic
Page 53 and 54: Evaluation of HA-binding and sperm
Page 55 and 56: HA-bound sperm and morphology with
Page 57 and 58: III. HA and ICSI outcome: prospecti
Page 59 and 60: HA- clinical uses and limitations
Page 61 and 62: May the force be with you Using imm
Page 63 and 64: Sperm ultrastructure Centrosome cha
Page 65 and 66: Diagnostic semen analysis Three ba
Page 67 and 68: Ultrastructural defects Transmissio
Page 69 and 70: Necrozoospermia / low viability rat
Page 71 and 72: 1. Hypo-osmotic swelling test ICSI
Page 73 and 74: 4. Laser-assisted selection (LAISS)
Page 75 and 76: 5. Birefringence-polarisation micro
Page 77 and 78: Conclusions Absolute asthenozoospe
Page 79 and 80: Definition of Azoospermia: Absence
Page 81 and 82: Cases of aspermia Preventive measur
Page 83 and 84: The probability crypto azoospermic
Page 85 and 86: "The beauty and the beast" when the
Page 87 and 88: Fertilization rate after ICSI 1- Oo
Page 89 and 90: With (ICSI), zona pellucida penetra
Page 91 and 92: Sibling oocytes from each patient w
Page 93 and 94: The electron microscopic results in
Page 95 and 96: Ionophore treatment for oocyte acti
Page 97 and 98:
The oocytes were suspended in 0.3M
Page 99 and 100:
Table 3. Pregnancy rates and outcom
Page 101 and 102:
References • Devroey P, Van Steir
Page 103 and 104:
Longing for a girl: Gender selectio
Page 105 and 106:
1,1 1 0,9 0,8 0,7 0,6 Sex ratio imb
Page 107 and 108:
PhD Thesis Michelle Duc, 1977 Retro
Page 109 and 110:
Reasons for wanting a girl at GC
Page 111 and 112:
Assessment of cervical mucus OOpaqu
Page 113 and 114:
Study design • A reference group
Page 115 and 116:
Partial data Reason n No post‐pre
Page 117 and 118:
Percentage P of girls 70 60 50 40 3
Page 119 and 120:
New aspects of this research • Fi
Page 121 and 122:
Mark your calendar for the upcoming
Page 123 and 124:
NOTES Page 121 of 127
Page 125 and 126:
Page 127 and 128:
Page 129:
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Beyond IUI, IVF and ICSI - New developments in - eshre 2013

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