Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
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Intracytoplasmic Morphologically Selected<br />
Sperm Injection<br />
Letter to <strong>New</strong> Engl<strong>and</strong> Journal of Medic<strong>in</strong>e:<br />
“Selection of spermatozoa with normal nuclei to improve the pregnancy rate with<br />
<strong>in</strong>tracytoplasmic sperm <strong>in</strong>jection”<br />
Bartoov et al. al (2001)<br />
Introduction of a new concept to observe spermatozoa called ‘motile‐sperm organelle‐<br />
morphology exam<strong>in</strong>ation’ (MSOME) <strong>and</strong> to evaluate the f<strong>in</strong>e nuclear morphology of motile<br />
spermatozoa <strong>in</strong> real time.<br />
Intracytoplasmic Morphologically Selected Sperm Injection<br />
(IMSI)<br />
IMSI: Sperm preparation<br />
‐ Use of a density gradient <strong>in</strong> the preparation prior to selection<br />
‐ Use of PVP (different concentration)<br />
‐low temperature (accord<strong>in</strong>g to sperm motility)<br />
Bartoov et al., 2002<br />
‐glass‐bottom dish over the top of an 100x objective lens covered by a droplet of<br />
immersion oil<br />
‐ Exam<strong>in</strong>ation of <strong>in</strong>dividual spermatozoa at high<br />
magnification by the <strong>in</strong>verted microscope<br />
equipped with high‐power nomarski optics<br />
enhanced by digital imag<strong>in</strong>g<br />
‐ sperm selection accord<strong>in</strong>g to MSOME criteria<br />
100x<br />
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