References -1 A<strong>in</strong>sworth CJ, Nixon B, Aitken RJ. The electrophoretic separation of spermatozoa: an analysis of genotype, surface carbohydrate composition <strong>and</strong> potential for capacitation. Int J Androl. 2011 Oct;34(5 Pt 2):e422-34. A<strong>in</strong>sworth C, Nixon B, Jansen RP, Aitken RJ. First recorded pregnancy <strong>and</strong> normal birth after <strong>ICSI</strong> us<strong>in</strong>g electrophoretically isolated spermatozoa. Hum Reprod. 2007 Jan;22(1):197-200. A<strong>in</strong>sworth C, Nixon B, Aitken RJ. Development of a novel electrophoretic system for the isolation of human spermatozoa. Hum Reprod. 2005 Aug;20(8):2261-70. A<strong>in</strong>sworth CJ, Nixon B, Aitken RJ. The electrophoretic separation of spermatozoa: an analysis of genotype, surface carbohydrate composition <strong>and</strong> potential for capacitation. Int J Androl. 2011 Oct;34(5 Pt 2):e422-34. Aitken RJ. Founders' Lecture. Human spermatozoa: fruits of creation, seeds of doubt. Reprod Fertil Dev. 2004;16(7):655-6 Aitken RJ, Krausz C. Oxidative stress, DNA damage <strong>and</strong> the Y chromosome. Reproduction. 2001 Oct;122(4):497-506. Aitk Aitken RJ RJ, CCurry BJ BJ. RRedox d regulation l ti off hhuman sperm ffunction: ti ffrom th the physiological h i l i l control t l off sperm capacitation it ti tto the etiology of <strong>in</strong>fertility <strong>and</strong> DNA damage <strong>in</strong> the germ l<strong>in</strong>e. Antioxid Redox Signal. 2011 Feb 1;14(3):367-81. Aitken RJ, De Iuliis GN, F<strong>in</strong>nie JM, Hedges A, McLachlan RI. Analysis of the relationships between oxidative stress, DNA damage <strong>and</strong> sperm vitality <strong>in</strong> a patient population: development of diagnostic criteria. Hum Reprod. 2010 Oct;25(10):2415-26. Aitken RJ, Hanson AR, Kuczera L. Electrophoretic sperm isolation: optimization of electrophoresis conditions <strong>and</strong> impact on oxidative stress. Hum Reprod. 2011 Aug;26(8):1955-64. Benchaib M, Braun V, Lornage J, Hadj S, Salle B, Lejeune H, Guér<strong>in</strong> JF.Sperm DNA fragmentation decreases the pregnancy rate <strong>in</strong> an assisted reproductive technique. Hum Reprod. 2003 May;18(5):1023-8. References - 2 Bungum M, Humaidan P, Spano M, Jepson K, Bungum L, Giwercman A. The predictive value of sperm chromat<strong>in</strong> structure assay (SCSA) parameters for the outcome of <strong>in</strong>trauter<strong>in</strong>e <strong>in</strong>sem<strong>in</strong>ation, <strong>IVF</strong> <strong>and</strong> <strong>ICSI</strong>. Hum Reprod. 2004 Jun;19(6):1401-8. Carrell DT, Liu L, Peterson CM, Jones KP, Hatasaka HH, Erickson L, Campbell B. Sperm DNA fragmentation is <strong>in</strong>creased <strong>in</strong> couples with unexpla<strong>in</strong>ed recurrent pregnancy loss. Arch Androl. 2003 Jan-Feb;49(1):49-55. Duran EH, Morshedi M, Taylor S, Oehn<strong>in</strong>ger S. Sperm DNA quality predicts <strong>in</strong>trauter<strong>in</strong>e <strong>in</strong>sem<strong>in</strong>ation outcome: a prospective cohort study. Hum Reprod. 2002 Dec;17(12):3122-8. Flem<strong>in</strong>g SD, Ilad RS, Griff<strong>in</strong> AM, Wu Y, Ong KJ, Smith HC, Aitken RJ Prospective controlled trial of an electrophoretic method of sperm preparation for assisted reproduction: comparison with density gradient centrifugation. Hum Reprod. 2008 Dec;23(12):2646-51. Greco E, Romano S, Iacobelli M, Ferrero S, Baroni E, M<strong>in</strong>asi MG, Ubaldi F, Rienzi L, Tesarik J. <strong>ICSI</strong> <strong>in</strong> cases of sperm DNA damage: beneficial effect of oral antioxidant treatment. Hum Reprod. 2005 Sep;20(9):2590-4. Hansen M, Bower C, Milne E, de Klerk N, Kur<strong>in</strong>czuk JJ. Assisted reproductive technologies <strong>and</strong> the risk of birth defects--a systematic review. Hum Reprod. 2005 Feb;20(2):328-38. Ji BT, Shu XO, L<strong>in</strong>et MS, Zheng W, Wacholder S, Gao YT, Y<strong>in</strong>g DM, J<strong>in</strong> F. Paternal cigarette smok<strong>in</strong>g <strong>and</strong> the risk of childhood cancer among offspr<strong>in</strong>g of nonsmok<strong>in</strong>g mothers. J Natl Cancer Inst. 1997 Feb 5;89(3):238-44. Loft S, Kold-Jensen T, Hjollund NH, Giwercman A, Gyllemborg J, Ernst E, Olsen J, Scheike T, Poulsen HE, Bonde JP. Oxidative DNA damage <strong>in</strong> human sperm <strong>in</strong>fluences time to pregnancy. Hum Reprod. 2003 Jun;18(6):1265-72. Morris ID, Ilott S, Dixon L, Brison DR. The spectrum of DNA damage <strong>in</strong> human sperm assessed by s<strong>in</strong>gle cell gel electrophoresis (Comet assay) <strong>and</strong> its relationship to fertilization <strong>and</strong> embryo development. Hum Reprod. 2002 Apr;17(4):990-8. References - 3 Sakkas D, Urner F, Bizzaro D, Manicardi G, Bianchi PG, Shoukir Y, Campana A.Sperm nuclear DNA damage <strong>and</strong> altered chromat<strong>in</strong> structure: effect on fertilization <strong>and</strong> embryo development. Hum Reprod. 1998 Dec;13 Suppl 4:11-9. Saleh RA, Agarwal A, Nada EA, El-Tonsy MH, Sharma RK, Meyer A, Nelson DR, Thomas AJ. Negative effects of <strong>in</strong>creased sperm DNA damage <strong>in</strong> relation to sem<strong>in</strong>al oxidative stress <strong>in</strong> men with idiopathic <strong>and</strong> male factor <strong>in</strong>fertility. Fertil Steril. 2003 Jun;79 Suppl 3:1597-605. Virro MR, Larson-Cook KL, Evenson DP. Sperm chromat<strong>in</strong> structure assay (SCSA) parameters are related to fertilization, blastocyst development, <strong>and</strong> ongo<strong>in</strong>g pregnancy <strong>in</strong> <strong>in</strong> vitro fertilization <strong>and</strong> <strong>in</strong>tracytoplasmic sperm <strong>in</strong>jection cycles. Fertil Steril. 2004 May;81(5):1289-95. Wisborg K, Ingerslev HJ, Henriksen TB. <strong>IVF</strong> <strong>and</strong> stillbirth: a prospective follow-up study. Hum Reprod. 2010 May;25(5):1312-6. Z<strong>in</strong>i A, Sigman M. Are tests of sperm DNA damage cl<strong>in</strong>ically useful? Pros <strong>and</strong> cons. J Androl. 2009 May-Jun;30(3):219-29. Page 30 of 127
ESHRE 2012: Pre‐congress course 2 www.generaroma.it CLINICA VALLE GIULIA, Rome SALUS, Marostica UMERTIDE, Perugia Magnificent? High‐power High power optical selection methods (IMSI vs <strong>ICSI</strong>) Laura Rienzi BSc MSc Senior Cl<strong>in</strong>ical Embryologist Laboratory Learn<strong>in</strong>g objectives 1) Sperm selection procedures prior to <strong>ICSI</strong> 2) Cl<strong>in</strong>ical outcomes related to IMSI approach 3) Sperm phenotype <strong>and</strong> sperm quality 4) Evidences to conclude on this issue I declare no commercial relationships or other activities that might be perceived as a potential conflict of <strong>in</strong>terest Male gamete abnormalities Embryos developmental problem Early events Late events Failed fertilization Abnormal zygote morphology Impaired cleavage Repeated implantation failures Increased pregnancy loss Page 31 of 127
- Page 1: 28th Annual Meeting of the European
- Page 5 and 6: Contents Course coordinators, cours
- Page 7 and 8: Course coordinators Herman Tournaye
- Page 9 and 10: Scientific programme Sperm selectio
- Page 11 and 12: ESHRE SIG Andrology, Istanbul, Turk
- Page 13 and 14: October 2001 - “A Physics Revelat
- Page 15 and 16: Polydimethylsiloxan and Sperm Survi
- Page 17 and 18: Microfluidic Sperm Isolation Reduce
- Page 19 and 20: Micro-insemination Device 1. Remove
- Page 21 and 22: Selecting spermatozoa; potential ro
- Page 23 and 24: Fertilization Rate (%) 120 100 80 6
- Page 25 and 26: Spermsep CS10 System Sperm Cell Sep
- Page 27 and 28: TUNEL (%) 14% 90 80 70 60 50 40 30
- Page 29 and 30: Cathode (- ve terminal) Anode (+ ve
- Page 31: Hydrogen peroxide mediates DNA dama
- Page 35 and 36: Sperm morphology and ICSI The estab
- Page 37 and 38: Motile Sperm Organellar Morphology
- Page 39 and 40: Conclusions of the meta‐analysis
- Page 41 and 42: Does the presence of sperm nuclear
- Page 43 and 44: Basic sperm parameters Sperm DNA fr
- Page 45 and 46: No relationship between chromosome
- Page 47 and 48: Gynecology: Filippo Ubaldi Elena Ba
- Page 49 and 50: Sperm selection: why? There is an i
- Page 51 and 52: I- MACS -Annexine V for diagnostic
- Page 53 and 54: Evaluation of HA-binding and sperm
- Page 55 and 56: HA-bound sperm and morphology with
- Page 57 and 58: III. HA and ICSI outcome: prospecti
- Page 59 and 60: HA- clinical uses and limitations
- Page 61 and 62: May the force be with you Using imm
- Page 63 and 64: Sperm ultrastructure Centrosome cha
- Page 65 and 66: Diagnostic semen analysis Three ba
- Page 67 and 68: Ultrastructural defects Transmissio
- Page 69 and 70: Necrozoospermia / low viability rat
- Page 71 and 72: 1. Hypo-osmotic swelling test ICSI
- Page 73 and 74: 4. Laser-assisted selection (LAISS)
- Page 75 and 76: 5. Birefringence-polarisation micro
- Page 77 and 78: Conclusions Absolute asthenozoospe
- Page 79 and 80: Definition of Azoospermia: Absence
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The probability crypto azoospermic
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"The beauty and the beast" when the
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Fertilization rate after ICSI 1- Oo
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With (ICSI), zona pellucida penetra
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Sibling oocytes from each patient w
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The electron microscopic results in
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Ionophore treatment for oocyte acti
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The oocytes were suspended in 0.3M
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Table 3. Pregnancy rates and outcom
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References • Devroey P, Van Steir
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Longing for a girl: Gender selectio
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1,1 1 0,9 0,8 0,7 0,6 Sex ratio imb
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PhD Thesis Michelle Duc, 1977 Retro
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Reasons for wanting a girl at GC
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Assessment of cervical mucus OOpaqu
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Study design • A reference group
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Partial data Reason n No post‐pre
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Percentage P of girls 70 60 50 40 3
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New aspects of this research • Fi
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Mark your calendar for the upcoming
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NOTES Page 121 of 127
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