Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
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Hydrogen peroxide mediates DNA damage<br />
2O 2<br />
2H + + 2O -.<br />
2 = O2 +H + H2O 2O 2<br />
Motility<br />
(%)<br />
Caspase activation<br />
But<br />
Impaired translocation of<br />
effectors of DNA cleavage such<br />
as Endo G, CAD <strong>and</strong> AIF<br />
This is why all DNA damage <strong>in</strong> the germ l<strong>in</strong>e is oxidative<br />
Pretreatment<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
Post-treatment<br />
Motility<br />
Impact of Vitam<strong>in</strong>s E <strong>and</strong> C on DNA damage<br />
** *** **<br />
Unit of meeasurement<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
Antioxidant<br />
***<br />
Density Motility Morphology TUNEL<br />
Progressive mootility<br />
(%)<br />
40<br />
35<br />
30<br />
25<br />
Progressive motility<br />
***<br />
**<br />
*<br />
Selection: TUNEL > 15%<br />
Impact of penicillam<strong>in</strong>e on sperm function<br />
0<br />
0 0.125 0.25 0.5 1.0<br />
Dose (mM)<br />
After 24 h <strong>in</strong>cubation at 37°C<br />
20<br />
15<br />
10<br />
5<br />
0<br />
0 0.125 0.25 0.5 1.0<br />
Dose (mM)<br />
(%)<br />
8OHdG<br />
25<br />
20<br />
2 months treatment<br />
Vit E (1g) <strong>and</strong> Vit C(1g)/day<br />
Greco et al., 2005<br />
Oxidative DNA damage<br />
30<br />
15<br />
10<br />
5<br />
** **<br />
0<br />
0 0.125 0.25 0.5 1.0<br />
Dose (mM)<br />
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