Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
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Pharmacological impacts on sperm function<br />
Critical to appreciate mechanisms <strong>in</strong>volved <strong>in</strong> causation of defective sperm function - particularly role<br />
of superoxide anion <strong>and</strong> hydrogen peroxide<br />
DHE<br />
Superoxide anion generation<br />
Eth<br />
H 2N<br />
Peak 1<br />
314<br />
NH2<br />
2OH Eth<br />
H 2N<br />
Et +<br />
Peak 2<br />
2OHEt +<br />
330 OH<br />
NH2<br />
Superoxide specific<br />
Lipid peroxidation generates powerful electrophiles<br />
acrole<strong>in</strong><br />
O 4-oxo-2 (E)-nonenal<br />
O<br />
O<br />
MSR (%)<br />
100<br />
80<br />
60<br />
40<br />
20<br />
Fe 2+<br />
Malondialdehyde<br />
O O<br />
*<br />
***<br />
0<br />
Pos 0 100 200 400 800<br />
*<br />
4-Hydroxynonenal (µM)<br />
O<br />
170<br />
130<br />
100<br />
70<br />
55<br />
40<br />
35<br />
25<br />
α-Tubul<strong>in</strong><br />
4-hydroxynonenal<br />
4HNE drives mitochondrial ROS generation<br />
4-Hydroxynonenal (µM)<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
0 10 20 30 40<br />
DHE Positive (%)<br />
R 2 = 0.85<br />
4-Hydroxynonenal (µM)<br />
15<br />
40<br />
30<br />
20<br />
10<br />
OH<br />
SDHA HNE<br />
0<br />
0 10 20 30 40 50 60<br />
MSR Positive (%)<br />
R 2 = 0.89<br />
SDHA/HNE<br />
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