Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
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m x 106 Chemilum<strong>in</strong>escence (cp )<br />
Electrophoretic isolation of spermatozoa<br />
100<br />
75<br />
50<br />
25<br />
Zymosan<br />
Leukocyte removal<br />
Media control<br />
0<br />
0 5 10 15 20<br />
Time (m<strong>in</strong>)<br />
Log chemilum<strong>in</strong>escence (cpm)<br />
Percoll<br />
8<br />
7<br />
6<br />
5<br />
4<br />
Residual sperm<br />
3<br />
2.5 3 3.5 4 4.5 5 5.5 6 6.5<br />
Log leukocyte concentration/ml<br />
E-Separated sperm<br />
Aitken <strong>and</strong> Curry, 2011<br />
Susceptibility of spermatozoa to oxidative stress<br />
Cumulative pregnnancy<br />
rate<br />
ROS<br />
High PUFA<br />
content<br />
R<br />
Hydrogen<br />
RH<br />
.<br />
R<br />
R Lipid Abstraction Lipid R<br />
radical<br />
Propagation<br />
Fe 2+<br />
Antioxidant<br />
Mechanisms<br />
Loss of membrane fluidity <strong>and</strong> function<br />
Lipid peroxidation<br />
O2 .<br />
ROO<br />
Limited<br />
Antioxidant<br />
CCapacity it<br />
ROOH<br />
ROS <strong>and</strong> Spontaneous Pregnancy<br />
Spontaneous pregnancy <strong>in</strong> 139 couples characterized by normal female partner<br />
0.6<br />
0.4<br />
0.2<br />
0.0<br />
0 10 20 30 40<br />
Months<br />
Redox activity<br />
(cpm x 10 4 )<br />
0-100<br />
101-1000<br />
1001-10000<br />
10001-<br />
50<br />
R<br />
Low ROS<br />
High fertility<br />
Intermediate ROS<br />
Intermediate fertility<br />
High ROS<br />
Low fertility<br />
Aitken <strong>and</strong> Curry, 2011<br />
Aitken <strong>and</strong> Curry, 2011<br />
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