Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
Beyond IUI, IVF and ICSI - New developments in - eshre 2013
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Spermsep CS10 System Sperm Cell Separation<br />
ation (106 Sperm concentra /ml)<br />
30<br />
25<br />
20<br />
15<br />
10<br />
5<br />
0<br />
Pros: Simple<br />
No contact with extraneous materials<br />
Rapid<br />
Effective with complex cellular mixtures<br />
Good yield<br />
Low levels of DNA damage<br />
Cons: Limited field trial<br />
Electrophoretic sperm isolation<br />
Sperm recovery<br />
30 60 120 300 600 900<br />
Duration of electrophoresis (sec)<br />
TUNEEL<br />
(%)<br />
20<br />
16<br />
12<br />
4<br />
DNA damage<br />
8 **<br />
0<br />
Orig<strong>in</strong>al Separated Residual<br />
Treatment<br />
Electrophoretic isolation of spermatozoa<br />
TUNEL posiitive<br />
(%)<br />
20<br />
15<br />
10<br />
5<br />
0<br />
TUNEL<br />
**<br />
Snap Frozen<br />
Reduction <strong>in</strong> DNA damage<br />
40<br />
35<br />
30<br />
25<br />
20<br />
15<br />
10<br />
5<br />
**<br />
Cryopreserved<br />
35<br />
30<br />
25<br />
20<br />
15<br />
10<br />
5<br />
Separated<br />
Residual<br />
**<br />
Biopsy<br />
A<strong>in</strong>sworth et al., 2005<br />
A<strong>in</strong>sworth et al., 2005<br />
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