Beyond IUI, IVF and ICSI - New developments in - eshre 2013

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Sperm Sorting Cumulus Removal Faculty Collaborator Shu Takayama, Ph.D. Thomas Pool, Ph.D. Thom Saunders, Ph.D. Integration: ART Lab on a Chip (Smith et al., 2012) Micro-insemination Embryo Analysis - metabolic/secretions - genetic Embryo Culture Detection Detection zone Zone Buffer Waste BW Enzyme mixture Mix Sample SW Waste Reference Reference Sample Buffer Mix ITO Heater heater Substrate Less Reagents/Cells More Physiological High Control/Automated Acknowledgements Financial Support NIH (NICHD, NGM) USDA MTTC Coulter Foundation References Laboratory Support Jun Ding, M.S. Yunseok Heo, Ph.D. Lula Cabrera, M.D. Tim Schuster, M.D. Ron Suh, M.D. Charlie Bormann, Ph.D. Jason Swain Swain, Ph Ph.D. D Nicole Acevedo, Ph.D. Brenda Cho, Ph.D. Terry Zhu, Ph.D. Pericles Hassun, Ph.D. Meghan Oakes, M.D. 1. Cho B, Schuster T, Zhu X, Chang D, Smith GD, Takayama S. Passively driven integrated microfluidic system for separation of motile sperm. Anal Chem (2003) 75:1671-1675. 2. Schuster TG, Cho B, Keller LK, Takayama S, Smith GD. Isolation of motile sperm from semen samples using microfluidics. Reprod BioMed (2003) 6:1-10. 3. Suh RS, Phadke N, Ohl DA, Takayama S, Smith GD. In vitro fertilization within microchannels requires lower total numbers and lower concentrations of spermatozoa. Human Reprod (2006) 21:477-483. 4. Smith GD, Takayama S, Swain JE. Rethinking in vitro embryo culture: new developments in culture platforms and potential to improve assisted reproductive technologies. Biol Reprod (2012) 86:1-10. Page 18 of 127
Selecting spermatozoa; potential roles for electrophoresis and pharmacological enhancement Laureate Professor R. John Aitken FRSE FAA Priority Research Centre in Reproductive Science Hunter Medical Research Institute and University of Newcastle Conflict of interest RJA is associated with a commercial company, NuSep, which is involved in the commercialization of electrophoretic methods for the preparation of human spermatozoa Selecting spermatozoa; potential roles for electrophoresis and pharmacological enhancement Learning objectives As a result of this lecture attendees should appreciate that: • Male infertility is a major cause of human infertility. • The male factor involves not just a compromised ability to fertilize oocyte but also an impaired capacity to support normal embryonic development. • Sperm preparation procedures should not only be rapid and efficient but should also select for cells exhibiting high levels of functionality and low levels of DNA damage. They should also eliminate leukocyte contamination. • Several methods for sperm isolation exist including swim-up, discontinuous gradient density centrifugation and electrophoresis. • Methods for pharmacologically enhancing the spermatozoa depend on a knowledge of the mechanisms responsible for defective sperm function. • Oxidative stress is a major factor in the aetiology of defective sperm function and antioxidants have some potential in the preservation of these cells. Clinical Problem Cyclees started Contribution of ART to Australasian population 60000 •Fertility in Australia is low, one in 50000 40000 30000 20000 10000 Year 4 woman remaining childless at the end of their reproductive lives. •One in 20 men are infertile •One in every 30 babies produced by ART •70,541 ART treatment cycles reported in Australia and New Zealand in 2009, a 13.9% increase on 2008 and a 48.0% increase on 2005 Page 19 of 127
Page 1: 28th Annual Meeting of the European
Page 5 and 6: Contents Course coordinators, cours
Page 7 and 8: Course coordinators Herman Tournaye
Page 9 and 10: Scientific programme Sperm selectio
Page 11 and 12: ESHRE SIG Andrology, Istanbul, Turk
Page 13 and 14: October 2001 - “A Physics Revelat
Page 15 and 16: Polydimethylsiloxan and Sperm Survi
Page 17 and 18: Microfluidic Sperm Isolation Reduce
Page 19: Micro-insemination Device 1. Remove
Page 23 and 24: Fertilization Rate (%) 120 100 80 6
Page 25 and 26: Spermsep CS10 System Sperm Cell Sep
Page 27 and 28: TUNEL (%) 14% 90 80 70 60 50 40 30
Page 29 and 30: Cathode (- ve terminal) Anode (+ ve
Page 31 and 32: Hydrogen peroxide mediates DNA dama
Page 33 and 34: ESHRE 2012: Pre‐congress course 2
Page 35 and 36: Sperm morphology and ICSI The estab
Page 37 and 38: Motile Sperm Organellar Morphology
Page 39 and 40: Conclusions of the meta‐analysis
Page 41 and 42: Does the presence of sperm nuclear
Page 43 and 44: Basic sperm parameters Sperm DNA fr
Page 45 and 46: No relationship between chromosome
Page 47 and 48: Gynecology: Filippo Ubaldi Elena Ba
Page 49 and 50: Sperm selection: why? There is an i
Page 51 and 52: I- MACS -Annexine V for diagnostic
Page 53 and 54: Evaluation of HA-binding and sperm
Page 55 and 56: HA-bound sperm and morphology with
Page 57 and 58: III. HA and ICSI outcome: prospecti
Page 59 and 60: HA- clinical uses and limitations
Page 61 and 62: May the force be with you Using imm
Page 63 and 64: Sperm ultrastructure Centrosome cha
Page 65 and 66: Diagnostic semen analysis Three ba
Page 67 and 68: Ultrastructural defects Transmissio
Page 69 and 70: Necrozoospermia / low viability rat
Page 71 and 72:
1. Hypo-osmotic swelling test ICSI
Page 73 and 74:
4. Laser-assisted selection (LAISS)
Page 75 and 76:
5. Birefringence-polarisation micro
Page 77 and 78:
Conclusions Absolute asthenozoospe
Page 79 and 80:
Definition of Azoospermia: Absence
Page 81 and 82:
Cases of aspermia Preventive measur
Page 83 and 84:
The probability crypto azoospermic
Page 85 and 86:
"The beauty and the beast" when the
Page 87 and 88:
Fertilization rate after ICSI 1- Oo
Page 89 and 90:
With (ICSI), zona pellucida penetra
Page 91 and 92:
Sibling oocytes from each patient w
Page 93 and 94:
The electron microscopic results in
Page 95 and 96:
Ionophore treatment for oocyte acti
Page 97 and 98:
The oocytes were suspended in 0.3M
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Table 3. Pregnancy rates and outcom
Page 101 and 102:
References • Devroey P, Van Steir
Page 103 and 104:
Longing for a girl: Gender selectio
Page 105 and 106:
1,1 1 0,9 0,8 0,7 0,6 Sex ratio imb
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PhD Thesis Michelle Duc, 1977 Retro
Page 109 and 110:
Reasons for wanting a girl at GC
Page 111 and 112:
Assessment of cervical mucus OOpaqu
Page 113 and 114:
Study design • A reference group
Page 115 and 116:
Partial data Reason n No post‐pre
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Percentage P of girls 70 60 50 40 3
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New aspects of this research • Fi
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Mark your calendar for the upcoming
Page 123 and 124:
NOTES Page 121 of 127
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Beyond IUI, IVF and ICSI - New developments in - eshre 2013

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